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Clean production method for neomycin sulfate fermentation

A neomycin sulfate and clean production technology, which is applied in fermentation and other fields, can solve the problems of high energy consumption and heavy pollution, and achieve the effects of stable ammonia nitrogen index, large steam consumption, and improved efficiency

Active Publication Date: 2020-02-28
YICHANG SANXIA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a clean production method of neomycin sulfate fermentation, which can solve the problems of large pollution and high energy consumption, and improve the carbon source class. The utilization rate of materials reduces the unit consumption of materials, thereby improving the level of fermentation units, while reducing energy consumption in the production process, reducing the concentration of ammonia nitrogen emissions by about 25% at the end of fermentation, and achieving clean production

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  • Clean production method for neomycin sulfate fermentation
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  • Clean production method for neomycin sulfate fermentation

Examples

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Effect test

Embodiment 1

[0035] A method for clean production of neomycin sulfate fermentation, the method comprises the following steps:

[0036] Step 1: Inoculate sandy soil spores to the slant, and prepare the slant spore suspension after cultivating at 28±1°C for 4 to 8 days;

[0037] Step 2: Put the slant spore suspension into a seed tank equipped with a seed medium, and cultivate for 35-40 hours to obtain a seed liquid;

[0038] Step 3: Connect the seed liquid into a fermenter equipped with a fermentation base medium, and ferment for 150 to 180 hours to obtain a fermentation liquid. During the fermentation process, add feed medium and ammonium sulfate to allow the accumulation of neomycin to reach Fermentation is terminated after the end point of the fermentation is required to obtain a fermented liquid,

[0039] Wherein, the fermentation basal medium includes 2% (W / V) maltodextrin, and the fermentation basal medium does not contain rice flour and α-amylase;

[0040] The feed medium includes 2...

Embodiment 2

[0054] A method for clean production of neomycin sulfate fermentation, the method comprises the following steps:

[0055] Step 1: Inoculate sandy soil spores to the slant, and prepare the slant spore suspension after cultivating at 28±1°C for 4 to 8 days;

[0056] Step 2: Put the slant spore suspension into a seed tank equipped with a seed medium, and cultivate for 35-40 hours to obtain a seed liquid;

[0057] Step 3: Connect the seed liquid into a fermenter equipped with a fermentation base medium, and ferment for 150 to 180 hours to obtain a fermentation liquid. During the fermentation process, add feed medium and ammonium sulfate to allow the accumulation of neomycin to reach Fermentation is terminated after the end point of the fermentation is required to obtain a fermented liquid,

[0058] Wherein, the fermentation basal medium includes 5% (W / V) maltodextrin, and the fermentation basal medium does not contain rice flour and α-amylase;

[0059] The feed medium includes 3...

Embodiment 3

[0073] A method for clean production of neomycin sulfate fermentation, the method comprises the following steps:

[0074] Step 1: Inoculate sandy soil spores to the slant, and prepare the slant spore suspension after cultivating at 28±1°C for 4 to 8 days;

[0075] Step 2: Put the slant spore suspension into a seed tank equipped with a seed medium, and cultivate for 35-40 hours to obtain a seed liquid;

[0076] Step 3: Connect the seed liquid into a fermenter equipped with a fermentation base medium, and ferment for 150 to 180 hours to obtain a fermentation liquid. During the fermentation process, add feed medium and ammonium sulfate to allow the accumulation of neomycin to reach Fermentation is terminated after the end point of the fermentation is required to obtain a fermented liquid,

[0077] Wherein, the fermentation basal medium includes 10% (W / V) maltodextrin, and the fermentation basal medium does not contain rice flour and α-amylase;

[0078] The feed medium includes ...

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Abstract

A clean production method for neomycin sulfate fermentation includes the following steps: step 1, preparing slant spore suspension liquid; step 2, preparing seed liquid; step 3, supplementing a supplementary culture medium and ammonium sulfate in a fermenting process to enable the accumulation amount of neomycin to reach a fermentation endpoint, and stopping fermentation to obtain fermenting liquid, wherein a fermentation basal culture medium comprises 2-10% (W / V) of maltodextrin and does not contain rice flour, starch and alpha-amylase, and the supplementary culture medium comprises 20-45% (W / V) of the maltodextrin and does not contain the rice flour, the starch and the alpha-amylase; step 4, pouring the fermenting liquid into an adsorption tank, and adding resin to adsorb the neomycin toobtain the finished neomycin sulfate. The clean production method for neomycin sulfate fermentation has the advantages that the problems of large pollution and high energy consumption can be solved,the utilization rate of high-carbon-source substances can be increased, and clean production can be realized.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a neomycin sulfate fermentation clean production method. Background technique [0002] In the fermentative production of neomycin sulfate, a large amount of rice flour and starch are used as fermentation carbon sources, especially rice flour as a grain raw material. Although it is rich in nutrients, carbohydrates account for 70-80%, and protein and amino acids account for about 10%. Its particle size is large and insoluble in water, and the utilization rate of microbial strains is low; at the same time, the production of neomycin sulfate mainly pursues the improvement of fermentation level, and a large amount of rice flour and starch hydrolyzed sugar solution are added, and the unit consumption of carbohydrates and its The conversion rate, when the fermentation ends, there are many residues, causing the main pollutant indicators to remain high, resulting in high p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/52
CPCC12P19/52
Inventor 谢飞胡珂崔国英谭兴龙王强江兵
Owner YICHANG SANXIA PHARMA
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