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Method for extracting and separating high-viscosity narcissus polysaccharides from narcissus bulbs

A narcissus bulb, high-viscosity technology, applied in the field of natural medicinal chemistry, can solve the problems of high production cost, high freeze-drying cost, starch gelatinization, etc.

Active Publication Date: 2020-02-21
嘉文丽(福建)化妆品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Like the above-mentioned patents, the use of hot water extraction is likely to cause a large amount of gelatinized starch in the prepared narcissus polysaccharide, and the cost of freeze-drying is very high
In addition, another disadvantage of using hot water extraction is that it is easy to cause the degradation of Narcissus polysaccharide, resulting in a serious decrease in the viscosity of the prepared product.
[0004] The technology provided by the above patents or documents has the following problems: (1) it needs to be degreased with ethanol or sherwood oil, then vacuum-dried or freeze-dried, and finally it can be pulverized, the process is complicated and time-consuming, and the production cost is higher; (2) ) using hot water for leaching, resulting in gelatinization of starch, which is difficult to separate from polysaccharides; (3) the temperature of hot water used in leaching is relatively high (80-100°C), which easily causes polysaccharides to degrade

Method used

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  • Method for extracting and separating high-viscosity narcissus polysaccharides from narcissus bulbs
  • Method for extracting and separating high-viscosity narcissus polysaccharides from narcissus bulbs
  • Method for extracting and separating high-viscosity narcissus polysaccharides from narcissus bulbs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment one: the preparation of narcissus bulb polysaccharide

[0047] (1) Take fresh narcissus bulbs, peel off the outer skin, cut off the root hairs, and rinse with clean water;

[0048] (2) Take 200 g of narcissus bulbs, add 400 mL of 0.05% potassium sorbate+0.1% EDTA aqueous solution, and beat with a homogenizer;

[0049] (3) Add 1600mL aqueous solution containing 0.05% potassium sorbate + 0.1% EDTA to the homogenate, and stir slowly for 30 minutes;

[0050] (4) filter with 200 mesh nylon cloth, collect filtrate;

[0051] (5) Adjust the pH to 3-4 with trichloroacetic acid, and stir at 15r / min for 30min;

[0052] (6) Centrifuge at 2000G for 30min, collect the supernatant;

[0053] (7) Adjust the pH of the supernatant to about 6 with alkali, and then concentrate it to 3-5 ° brix with a rotary evaporator;

[0054] (8) Add 3 times of alcohol to the concentrated solution, stir slowly, let it stand for 60 minutes, filter and collect the precipitate with 100 mesh ny...

Embodiment 2

[0056] Embodiment two: the preparation of narcissus bulb polysaccharide

[0057] (1) Take fresh narcissus bulbs, peel off the outer skin, cut off the root hairs, and rinse with clean water;

[0058] (2) Get 2 kg of narcissus bulbs, shred them, and put them into extraction tanks;

[0059] (3) Add 10kg containing 0.05% potassium sorbate+0.1% EDTA aqueous solution in the extraction tank, and stir for 120min, discharge; Leach again 2 times, each solvent is 5kg;

[0060] (4) Combine the extracts, filter with 200 mesh nylon cloth, and collect the filtrate;

[0061](5) Adjust the pH to 3-4 with trichloroacetic acid, and stir at 30r / min for 30min;

[0062] (6) Centrifuge with a tubular centrifuge to collect the light phase;

[0063] (7) Adjust the pH of the light phase to about 6 with alkali, and then concentrate it to 3-5 ° brix with a falling film evaporator;

[0064] (8) Add 3 times of alcohol to the concentrated solution, stir slowly for 90 minutes, filter and collect the prec...

Embodiment 3

[0066] Embodiment three: the preparation of narcissus bulb polysaccharide

[0067] (1) Take fresh narcissus bulbs, peel off the outer skin, cut off the root hairs, and rinse with clean water;

[0068] (2) get narcissus bulb 100kg, cross extruder, drop into extraction tank;

[0069] (3) Add 1000kg containing 0.05% potassium sorbate+0.1% EDTA aqueous solution to the leaching tank, and stir for 120min, discharge; leaching again 1 time, the solvent is 800kg;

[0070] (4) Combine the extracts, filter with a filter bag of 200 mesh to 400 mesh, and collect the filtrate;

[0071] (5) Adjust the pH to 3-4 with citric acid, and stir at 60r / min for 30min;

[0072] (6) Centrifuge with a tubular centrifuge to collect the light phase;

[0073] (7) Adjust the pH of the light phase to about 6 with alkali, and then concentrate it to 3-5 ° brix with a falling film evaporator;

[0074] (8) Add 2.5 times of alcohol to the concentrated solution, stir slowly for 120 minutes, then filter and col...

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Abstract

The invention belongs to the technical field of extraction and separation of active ingredients of natural plants, and particularly relates to a method for extracting and separating high-viscosity narcissus polysaccharides from narcissus bulbs. The narcissus bulbs can secrete a mucilage liquid when being bitten by insects and subjected to other physical injuries, and the main component of the mucilage liquid is macromolecular polysaccharide narcissus polysaccharide. The invention provides a method for extracting and separating narcissus polysaccharide, wherein the narcissus polysaccharide obtained through the method is high in viscosity and free of starch and alkaloid residues.

Description

technical field [0001] The invention belongs to the technical field of natural medicine chemistry and relates to a technology for extracting and separating natural plant active ingredients, in particular to a method for extracting and separating high-viscosity narcissus polysaccharides from narcissus bulbs. Background technique [0002] Narcissus tazetta L. is a perennial herbaceous plant of the genus Narcissus in the family Amaryllidaceae. The flowers are fragrant and elegant, and have high ornamental value. It is one of the ten traditional famous flowers in China. Narcissus multiflora is planted in Fujian, Jiangsu, Zhejiang and other places in my country. Among them, the Narcissus multiflora in Zhangzhou is the most famous and has the largest planting area. According to literature reports [1], the bulbs of narcissus contain a variety of biologically active chemical substances, such as alkaloid compounds such as narcissine and pseudolycorine, sugars such as glucose, mannose...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 饶焕文林建平萧自智潘发伍林晓锋
Owner 嘉文丽(福建)化妆品有限公司
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