New method for extracting euscaphic acid from plant body, and new raw material for euscaphic acid extraction

Abstract

The invention provides a new method for extracting euscaphic acid from a plant body, and a new raw material for euscaphic acid extraction. The plant body containing euscaphic acid, which is used as araw material, is crushed, and undergoes extraction by using an ethyl acetate solution, the obtained extract solution is concentrated to form an extract, the extract undergoes silica gel column chromatography elution, and the obtained eluate undergoes PRP512A macro-porous adsorption resin column chromatography separation, and is concentrated and recrystallized to obtain the euscaphic acid monomericcompound. The method has the advantages of avoiding of multi-time silica gel and gel column chromatography separation, simple process, high yield and low cost; and the Euscaphis konishii Hayata plantbody has the advantages of rich raw material source and high content, and is an ideal new material.

Description

technical field [0001] The patent of the present invention relates to a new method for extracting ravenic acid from plants, and a new raw material for extracting ravenic acid. Background technique [0002] Yaracic acid, also known as rosic acid, CAS number 53155-25-2, English name euscaphic acid, white powder crystal or needle crystal, molecular formula is C 30 h 48 o 5 , Molecular weight 488; The compound is an ursane-type triterpenoid compound, soluble in organic solvents such as methylene chloride, ethyl acetate, and chloroform. It has been reported in the literature that ravenic acid has significant anti-HIV, anti-inflammatory, and anti-tumor activities, and its market development value is huge. [0003] The ingredient was first obtained from the province's Guyoukei Yacao Euscaphis japonica isolated from the peel of the fruit. Literature research shows that ravenic acid is present in plants such as the family of oil family, Rosaceae, and theaceae, and has been isola...

AI Technical Summary

Problems solved by technology

Literature research shows that ravenic acid is present in plants such as the family of oil family, Rosaceae, and theaceae, and has been isolated from plants such as Arvenia, Rubus raspberry, Burnet, and loquat leaves. The process is complicated and the form Variety, limited to the preparation of a certain plant species of ravenic acid; among the many processes, the most common one is from the She nationality medicine, that is, the East China raspberry Rubus chingii Hu's dried roots and rhizomes have the highest yield of bulrush acid, with a yield of about 1%. However, it needs to be extracted-extracted-repeatedly separated by silica gel and gel column chromatography. The separation method is cumbersome, and the ingredients are prepared The amount of loss in the process is large, and the column chromatography filler dextran gel used in the process is expensive, and the cost of the preparation process is high; this process prepares ravenic acid in other plants, and the obtained ravenic acid is small in amount and low in purity

In addition, although the East China raspberry used in this process is rich in sources, it is limited to roots and rhizomes, and its biomass in the plant is low and its development potential is small.