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Wavelength/angle modulation free conversion polarized fluorescence imaging surface plasmon resonance instrument

A technology of surface plasmon and angle modulation, applied in fluorescence/phosphorescence, instruments, scientific instruments, etc., can solve the problems of increasing the cost of anisotropic samples, high cost of confocal parts, and limited application range, etc. The effect of noise ratio and detection sensitivity, simple structure and easy operation

Pending Publication Date: 2020-02-18
长春国科希莱科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no commercial product integrating polarized fluorescence and wavelength / angle modulation surface plasmon resonance technology at home and abroad.
Although there are surface plasmon resonance devices based on laser confocal imaging with application number 200710177653.7 and surface plasmon resonance with polarization modulation laser confocal imaging with application number 201110436241.7 in the previous inventions, the cost of the confocal part is high, which greatly increases the detection of anisotropic samples. The cost, and the scope of application is limited by the single modulation method of surface plasmon resonance

Method used

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Embodiment 1

[0025] figure 1 It shows the structure of the wavelength / angle modulation free conversion polarization fluorescence imaging surface plasmon resonator according to the first embodiment of the present invention.

[0026] Such as figure 1 with figure 2 As shown together, the wavelength / angle modulation free conversion polarized fluorescence imaging surface plasmon resonance instrument provided by the first embodiment of the present invention includes: a polarized upright fluorescence microscope system 1, a three-dimensional sample stage 2, a cage type incident light path 3, a cage Type reflective optical path 4, electromechanical corner device, data processing system and multi-channel syringe pump 6; among them, the polarized upright fluorescence microscope system 1 is used to carry out the reaction process between the anisotropic biological samples on the three-dimensional sample stage 2 Polarized light microscopy imaging and send it to the data processing system, the cage inciden...

Embodiment 2

[0040] The photodetector 403' adopts, for example, a photomultiplier tube with a model of 9863 / 100B, and the others are the same as the first embodiment. Compared with the first embodiment, the sensitivity and detection accuracy of the wavelength / angle modulation free conversion polarization fluorescence imaging surface plasmon resonator provided by the second embodiment can be improved by two orders of magnitude.

Embodiment 3

[0042] The reflective film on the surface of the sample substrate 202 adopts Au(45nm) / [TiO2(20nm) / ITO(20nm)]4 / Au(20nm) composite film or Au(25nm) / [TiO2(20nm) / SiO2(20nm)] 2 / Au (30nm) composite film, the others are the same as the first embodiment. Compared with the first embodiment, the sensitivity and detection accuracy of the wavelength / angle modulation free conversion polarization fluorescence imaging surface plasmon resonator provided by the third embodiment can be improved by 1-2 orders of magnitude.

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Abstract

The invention provides a wavelength / angle modulation free conversion polarized fluorescence imaging surface plasmon resonance instrument comprising a polarized upright fluorescence microscopy opticalpath, a sample three-dimensional stage, a cage incident optical path, a cage reflected optical path, an electromechanical turning device, a data processing system and a syringe pump, wherein the sample three-dimensional stage is integrated with a right-angle prism, a gold-plated sample substrate and a multi-channel microfluidic flow cell; and the liquid inlet and outlet of the multi-channel microfluidic flow cell are respectively connected to the liquid outlet and inlet of the syringe pump through pipelines. The wavelength / angle modulation free conversion polarized fluorescence imaging surfaceplasmon resonance instrument provided by the invention can realize real-time observation through polarized fluorescence imaging at the same time when performing high-sensitivity quantitative and qualitative wavelength angle modulation free conversion surface plasmon resonance detection on the interaction between anisotropic biological samples, and the refractive index changes up to 1.0*10-5, thedetection sensitivity reaches 10-11g / ml, and the image resolution is up to 2um. The wavelength / angle modulation free conversion polarized fluorescence imaging surface plasmon resonance instrument provided by the invention takes into account the standing balance training modes at all levels, and satisfies the needs of the patient group for standing balance and stable training at all stages.

Description

Technical field [0001] The present invention relates to the technical field of multi-channel detection of biomolecular reactions, and more specifically, to the interaction of real-time observation based on a polarized fluorescence microscopy system and a wavelength / angle modulation free conversion surface plasmon resonance multi-channel microfluidic detection technology The fluorescent polarized imaging surface plasmon resonance instrument. Background technique [0002] In life science research, the interaction between biomolecules is a basic life phenomenon, and it is also one of the major issues in modern life science research. There are many traditional methods to study the interaction between biomolecules, such as radioimmunity. Analysis methods, enzyme-linked immunoassay methods, labeling and tracing methods, etc. However, because these methods involve different types and contents of cells and biomolecules, and there are complex interactions between various substances, it i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6458G01N21/6445
Inventor 张洪艳马海涛张秀芹张美玲
Owner 长春国科希莱科技有限公司
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