Method for detecting content of urushiol in fruit of Rhus chinensis Mill.

A technology of salt skin wood and fruit, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as unreported

Inactive Publication Date: 2020-02-14
WEST ANHUI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the reported detection of urushiol is mainly the detection of the high content of urushiol in the sumac tree, but the detection of the trace amount of urushiol in the fruit of Salmonella has not been reported.

Method used

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  • Method for detecting content of urushiol in fruit of Rhus chinensis Mill.
  • Method for detecting content of urushiol in fruit of Rhus chinensis Mill.
  • Method for detecting content of urushiol in fruit of Rhus chinensis Mill.

Examples

Experimental program
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Effect test

Embodiment 1

[0049] Supercritical CO of urushiol in the fruit of Salmonella chinensis 2 The extraction steps are as follows:

[0050] Drying the fruit of Salmonella, crushing it to 80 mesh;

[0051] Loading of Salmonella fruit powder into CO 2 In the charging barrel of the extraction tank, run supercritical CO 2 Extraction equipment, the extraction temperature is set to 42°C, the extraction pressure is set to 23MPa; the continuous extraction is 2.2h, and the extracted oil is released from the separation I separation tank every 28min, the separation I temperature is 52°C, the separation I pressure is 9MPa, and the temperature is balanced to room temperature, CO 2 After the exhaustion is exhausted, the liquid sarcocarp fruit oil is obtained;

[0052] Supercritical CO 2 Pump absolute ethanol into the extraction instrument and run supercritical CO 2 Extraction equipment, the extraction temperature is set to 42°C, the extraction pressure is set to 23MPa; the continuous extraction is 2.2h,...

Embodiment 2

[0054] Supercritical CO of urushiol in the fruit of Salmonella chinensis 2 The extraction steps are as follows:

[0055] Drying the fruit of Salmonella, crushing it to 90 mesh;

[0056] Loading of Salmonella fruit powder into CO 2 In the charging barrel of the extraction tank, run supercritical CO 2 Extraction equipment, the extraction temperature is set to 38°C, the extraction pressure is set to 27MPa; the continuous extraction is 1.8h, and the extracted oil is released from the separation I separation tank every 32min, the separation I temperature is 48°C, the separation I pressure is 11MPa, and the temperature is balanced to room temperature, CO 2 After the exhaustion is exhausted, the liquid sarcocarp fruit oil is obtained;

[0057] Supercritical CO 2 Pump absolute ethanol into the extraction instrument and run supercritical CO 2 Extraction equipment, the extraction temperature is set to 38°C, the extraction pressure is set to 27MPa; the continuous extraction is 1.8h...

Embodiment 3

[0059] Supercritical CO of urushiol in the fruit of Salmonella chinensis 2 The extraction steps are as follows:

[0060] Sun-dried the fruit of Salmonella, stored in a -20°C freezer, taken out and dried before extraction, and crushed to 100 meshes;

[0061] Loading of Salmonella fruit powder into CO 2 In the charging barrel of the extraction tank, run supercritical CO 2 Extraction equipment, the extraction temperature is set to 40°C, the extraction pressure is set to 25MPa; continuous extraction for 2 hours, release the extracted oil from the separation I separation tank every 30min, the separation I temperature is 50°C, the separation I pressure is 10MPa, and the temperature is balanced to Room temperature, CO 2 After being wiped away, the liquid sarcocarp fruit oil is obtained.

[0062] Salmonia fruit powder mass (M) is 401.4g, liquid Salina fruit oil quality (m) is 62.82g, Salmonia fruit oil volume (V 1 ) was 57.1mL, the fruit oil yield (R) of Salmonella fruit was 15.6...

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Abstract

The invention discloses a method for detecting the content of urushiol in a fruit of Rhus chinensis Mill. The fruit of Rhus chinensis Mill. is subjected to supercritical CO2 extraction, and then is determined by adopting a combination technology of ultra-high performance liquid chromatography-mass spectrometry. The method adopts a supercritical CO2 extraction-ultra-high performance liquid chromatography-mass spectrometry (SCFE-UPLC-MS) combination technology method to perform qualitative and quantitative determination on urushiol in the fruit of Rhus chinensis Mill. A working principle of themethod is as follows: after urushiol in the fruit of Rhus chinensis Mill. is extracted by adopting optimum conditions for extracting urushiol in a supercritical CO2 extraction method, saturated urushiol is used as a standard control, and ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS) is adopted to determine the type and content of urushiol in a CO2 extract of the fruit ofRhus chinensis Mill. and in an ethanol carrying agent, and finally, the sum of the contents of two parts of saturated urushiol and various unsaturated urushiols is converted into the content of totalurushiol in the fruit of Rhus chinensis Mill.

Description

technical field [0001] The invention relates to the technical field of trace detection, in particular to a method for detecting the urushiol content in the fruit of A. 2 Extraction-ultra high performance liquid chromatography-mass spectrometry method for the detection of urushiol in the fruits of Salmonella chinensis. Background technique [0002] Salt skin wood (Rhus chinensis Mill.), also known as gallnut tree (Sycamore chinensis, yellow flesh tree, bully tree) is one of the common economic tree species in China, with a wide distribution area and strong adaptability. The gallnuts parasitic on the leaves of Salskinia can be used as raw materials for pharmaceuticals and industrial dyes, and its fruits and seeds can also be used for oil extraction. In landscaping, it can be used as one of the tree species for viewing leaves and fruits. Flowers bloom from August to September, rich in honey and powder, and are good nectar source plants. Roots, leaves, flowers and fruits can ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 闵运江陈存武许正刚闵长莉汪学军王广林陈乃东乔德亮邓辉陈瀚
Owner WEST ANHUI UNIV
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