Primer group for visual detection of MCR-3 polymyxin drug resistance gene, and detection method

Abstract

The invention provides a primer group for visual detection of an MCR-3 polymyxin drug resistance gene, and a detection method, and belongs to the field of detection of bacterial drug resistance genes.According to the technical scheme, the primer group comprises an outer primer F3, an outer primer B3, an inner primer FIP and an inner primer BIP; an LAMP amplification reaction is carried out on a DNA sample at 62 DEG C, an appropriate amount of hydroxy naphthol blue (HNB) is added into an LAMP reaction system in advance, and whether the MCR-3 gene exists in a to-be-detected object or not is judged by observing the color change of reaction liquid. The primer group and the detection method have the beneficial effects that the MCR-3 gene can be quickly and efficiently detected with high specificity and high sensitivity under the condition of constant temperature, expensive and complex instruments are not needed, the new method is provided for detection of the bacterial drug resistance genes, and great application value is achieved.

Description

technical field [0001] The invention relates to the field of detection of bacterial drug-resistant genes, in particular to a primer set for visual detection of MCR-3 polymyxin drug-resistant genes and a detection method thereof. Background technique [0002] Since the 1920s, a large number of natural antibiotics have been discovered one after another, opening the era of antibiotics, but with the over-reliance on antibiotics, the problem of bacterial drug resistance has become increasingly prominent. Resistant bacteria make antibiotics less effective or even ineffective. Polymyxins (Polymyxins) is a group of polypeptide antibiotics extracted from the culture medium of Polymyxins, which has 5 components (A, B, C, D, E). Clinically commonly used are polymyxin (PolymyxinB) and polymyxin E (colislin, anti-enemy). Polymyxin is considered to be the last line of defense against bacteria; [0003] In recent years, the rapid evolution of the plasmid-mediated colistin resistance gen...

AI Technical Summary

Problems solved by technology

[0004] Due to the widespread existence of MCR-3, especially in healthy people and animal foods, the detection procedures and methods currently used for MCR detection, such as selective bacterial screening, mass spectrometry identification of bacterial species, drug sensitivity detection and ordinary PCR verification, have changed. cumbersome and inefficient

It has not been widely used clinically due to different reasons such as the need for expensive instruments and equipment.

Images