Sugar-free culture and strong seedling transplanting method for virus-free Rehmannia glutinosa seedlings
A technology of sugar-free cultivation and virus-free seedlings, which is applied in the field of plant tissue culture, can solve the problems of microbial contamination transplanting survival rate, poor growth quality of Rehmannia glutinosa seedlings, etc., and achieve good water retention performance, good air permeability, and good plant growth Effect
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Embodiment 1
[0031] A sugar-free culture and strong seedling transplanting method of Rehmannia glutinosa virus-free seedlings comprises the following steps:
[0032] (1) Carry out the detoxification tissue culture of Rehmannia glutinosa in a test tube, multiply the detoxified seedlings, select the detoxified seedlings grown for 35 days, cut off the old roots, cut into 3cm stems, and transplant them to the sugar-free culture with vermiculite In the box, each box contains 670g vermiculite, pour 800mL nutrient solution, transplant 80 seedlings in each box, cover the box cover and seal the gap between the box cover and the box body with sealing film; the nutrient solution contains 2g / L nitric acid Calcium ammonium and 170mg / LKH 2 PO 4 , The rest is deionized water;
[0033] (2) After transplanting, put it on the culture rack in a closed room, pass the CO 2 The controller passes CO into the culture box 2 , Open the ventilation hole of the culture box, no CO for the first 4 days during the culture pe...
Embodiment 2
[0041] A sugar-free culture and strong seedling transplanting method of Rehmannia glutinosa virus-free seedlings comprises the following steps:
[0042] (1) Carry out the detoxification tissue culture of Rehmannia glutinosa in a test tube, multiply the detoxified seedlings, select the detoxified seedlings grown for 35 days, cut off the old roots, cut into 3cm stems, and transplant them to the sugar-free culture with vermiculite In the box, each box contains 670g vermiculite, pour 800mL nutrient solution, transplant 80 seedlings in each box, cover the box cover and seal the gap between the box cover and the box body with sealing film; the nutrient solution contains 2g / L nitric acid Calcium ammonium and 170mg / LKH 2 PO 4 , The rest is deionized water;
[0043] (2) After transplanting, put it on a culture rack in a closed room, and let CO into the culture box 2 , Open the ventilation hole of the culture box, no CO for the first 4 days during the culture period 2 Gas supply, start CO on...
Embodiment 3
[0049] A sugar-free culture and strong seedling transplanting method of Rehmannia glutinosa virus-free seedlings comprises the following steps:
[0050] (1) Carry out the detoxification tissue culture of Rehmannia glutinosa in a test tube, multiply the detoxified seedlings, select the detoxified seedlings grown for 35 days, cut off the old roots, cut into 3cm stems, and transplant them to the sugar-free culture with vermiculite In the box, each box contains 670g vermiculite, pour 800mL nutrient solution, transplant 80 seedlings in each box, cover the box cover and seal the gap between the box cover and the box body with sealing film; the nutrient solution contains 2g / L nitric acid Calcium ammonium and 170mg / LKH 2 PO 4 , The rest is deionized water;
[0051] (2) After transplanting, put it on a culture rack in a closed room, and let CO into the culture box 2 , Open the ventilation hole of the culture box, no CO for the first 4 days during the culture period 2 Gas supply, start CO on...
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