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A method for reducing the deformity rate of kelp germplasm parthenogenetic sporophytes

A technology of young sporophytes and kelp, which is applied in the field of kelp breeding, can solve the problems of high parthenogenetic deformity rate, environmental conditions that do not meet the special requirements of parthenogenetic growth, chromosome loss, etc., and achieve shortened ovulation time, good synchronization, and ovulation high rate effect

Active Publication Date: 2022-02-15
MARINE BIOLOGY INST OF SHANDONG PROVINCE
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Problems solved by technology

However, the deformity rate of parthenogenesis is very high, generally reaching about 80%. The environmental conditions of cultivation do not meet the special requirements of parthenogenetic growth

Method used

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  • A method for reducing the deformity rate of kelp germplasm parthenogenetic sporophytes
  • A method for reducing the deformity rate of kelp germplasm parthenogenetic sporophytes
  • A method for reducing the deformity rate of kelp germplasm parthenogenetic sporophytes

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Embodiment 1

[0032] Embodiment 1: promote kelp germplasm to discharge ovum

[0033] 1) Prepare germplasm material for parthenogenetic development

[0034] The female germplasm filaments of kelp (Saccharina japonica) with dark brown color and no algae pollution were selected from the germplasm bank, and part of the germplasm was poured out for induction materials. Those used to induce germplasm were placed in 500ml sterile sea water.

[0035] 2) Pre-treatment for induction

[0036] The germplasm used for induction was placed in an induction incubator 24 hours before induction, the culture temperature was 15±1°C, the light source was a fluorescent tube, and the light was 1000lx, increasing by 500lx per hour until 6000±200lx.

[0037] 3) Ovulation induction treatment

[0038] The germplasm was crushed with a tissue grinder at 1500 rpm for 30 seconds, and repeated once. Then filter three times with a single-layer 500-mesh sieve to obtain filament fragments with a length of 3-6 cells.

[0...

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Abstract

The present invention firstly provides a method for promoting kelp female gametophytes to discharge eggs. First, the kelp germplasm filaments are recovered in seawater, then the kelp germplasm is crushed, and the crushed liquid is filtered to obtain cells with a length of 3-6. The obtained filamentous body fragments; then the obtained filamentous body fragments were subjected to aerated suspension culture under the conditions of light intensity 6000±300lx, light cycle: L:D=12h:12h, and temperature 15±1°C to collect eggs; and then The collected eggs are cultured to obtain laminar sporozoites, and the laminar sporozoites are cultured, and the young sporophytes of the two rows of cells are cultured in flowing water. The method of the present invention enables the kelp female germplasm to ovulate in 11-13 days, shortening the ovulation time; and the ovulation rate is high and well synchronized, and the ovulation rate can reach 65-70% within 2 days; the egg development rate can reach 70-80% %. The established young sporophyte culture method can reduce the deformity rate of the young sporophyte to 50%-60%.

Description

technical field [0001] The invention belongs to the technical field of kelp breeding, and in particular relates to a method for reducing the parthenogenic deformity rate of kelp female germplasm. Background technique [0002] Kelp is a large-scale economic brown alga with heterogeneous generation alternation. The life history of natural growth of kelp includes short-term gametophytes and long-term sporophyte stages. Mature kelp sporophytes release to produce zoospores. After the zoospores attach, they develop into female and male gametophytes, and the gametophytes respectively Sperm eggs are produced, the eggs are fertilized to form zygotes, and the zygotes develop into young sporophytes. But there is also a parthenogenetic pathway in which the female germplasm releases eggs and the eggs split into young sporophytes in kelp. [0003] Parthenogenesis is of great significance for breeding. If it can develop into adults, it can accelerate the homozygous speed of offspring and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G33/00A01H4/00
CPCA01G33/00A01H4/001A01H4/005
Inventor 王翔宇辛美丽吕芳吴海一詹冬梅
Owner MARINE BIOLOGY INST OF SHANDONG PROVINCE
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