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Preparation method of nano microspheres with biological activity

A nano-microsphere and biologically active technology, which is applied in the field of preparation of nano-microspheres, can solve the problems of poor drug-loading properties of nano-microspheres and the inability to achieve targeted drug delivery, and achieve good drug-loading and targeted drug delivery effects

Inactive Publication Date: 2020-01-24
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that when sodium alginate is used as the main raw material to prepare gels, it is necessary to cooperate with templates, molds, etc. for preparation, nano-microspheres cannot be prepared simply and efficiently, and some nano-microspheres have poor drug-loading properties and cannot achieve targeted drug delivery, etc. Problem, the present invention provides a preparation method of biologically active nano-microspheres

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A method for preparing biologically active nano-microspheres, the method comprising the following steps:

[0039] Dissolve sodium alginate and polyguluronic acid with a molar ratio of 1:0.1 in deionized water, add an activator for activation to obtain an activation solution, the molar ratio of sodium alginate to activator is 1:1.75, and the activator consists of Carbodiimide hydrochloride and N-hydroxysuccinimide are composed of a molar ratio of 1:1, the concentration of sodium alginate in the activation solution is 6wt%, and the activation solution is mixed with 1.0wt% calcium chloride solution by volume Mix at a ratio of 1:2.5, then add aminomercaptool, the molar ratio of sodium alginate to aminomercaptool is 1:1, and place it in the air atmosphere for self-crosslinking to obtain biologically active nanospheres.

Embodiment 2

[0041] A method for preparing biologically active nano-microspheres, the method comprising the following steps:

[0042] Sodium alginate and polyguluronic acid with a molar ratio of 1:0.15 were dissolved in deionized water, and an activator was added for activation to obtain an activation solution. The molar ratio of sodium alginate and activator was 1:1, and the activator was prepared by Carbodiimide hydrochloride and N-hydroxysuccinimide are composed of a molar ratio of 1:1.5, the concentration of sodium alginate in the activation solution is 5wt%, and the activation solution is mixed with 1.0wt% calcium chloride solution by volume Mix at a ratio of 1:2, then add aminomercaptool, the molar ratio of sodium alginate to aminomercaptool is 1:1, and place it in the air atmosphere for self-crosslinking to obtain biologically active nanospheres.

Embodiment 3

[0044] A method for preparing biologically active nano-microspheres, the method comprising the following steps:

[0045] Dissolve sodium alginate and polyguluronic acid with a molar ratio of 1:0.15 in deionized water, add an activator for activation to obtain an activation solution, the molar ratio of sodium alginate to activator is 1:1.5, and the activator consists of Carbodiimide hydrochloride and N-hydroxysuccinimide are composed of a molar ratio of 1:1.5, the concentration of sodium alginate in the activation solution is 5wt%, and the activation solution is mixed with 1.0wt% calcium chloride solution by volume Mix at a ratio of 1:2.5, then add aminomercaptool, the molar ratio of sodium alginate to aminomercaptool is 1:1, and place it in the air atmosphere for self-crosslinking to obtain biologically active nanospheres.

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PUM

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Abstract

The invention relates to the field of nano gel materials, in particular to a preparation method of nano microspheres with biological activity. The method comprises the following steps: dissolving sodium alginate and polyguluronic acid in a solvent, adding an activator for activation to obtain an activation solution, mixing the activation solution with a calcium salt solution, adding aminomercaptan, and carrying out self-crosslinking in an oxygen-containing atmosphere to obtain the nano microspheres with biological activity. The preparation method is simple and efficient; the nano microspherescan be directly prepared by a one-step method; the prepared nano microsphere has good drug loading property and targeted drug delivery property; under the matching effect of polyguluronic acid, the nano microspheres have the capability of stimulating the immune response of organisms.

Description

technical field [0001] The invention relates to the field of nano gel materials, in particular to a method for preparing nano microspheres with biological activity. Background technique [0002] Sodium alginate is the sodium salt of a natural polyanionic polysaccharide alginic acid extracted from brown algae, which is connected by M units (mannuronic acid) and G units (guluronic acid) in the form of 1,4-glycosidic bonds , and a random block copolymer composed of a certain proportion of MM, GM, and GG segments. Polyguluronic acid is an oligomerization product of alginate, which has the function of activating macrophages. With polyguluronic acid, under the action of calcium ions and cross-linking agents, tumor-targeted nanospheres can be prepared, which can not only Loaded with chemotherapy drugs, the matrix itself is decomposed into oligomeric guluronic acid at the tumor site, which also has the function of activating the immune system in the tumor microenvironment. [0003...

Claims

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Application Information

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IPC IPC(8): A61K9/16A61K47/36A61K31/702A61K31/715A61P37/04
CPCA61K9/1652A61K31/702A61K31/715A61P37/04
Inventor 陈荫王腾董喆孙坤来赵玉勤王斌
Owner ZHEJIANG OCEAN UNIV
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