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Micromeria biflora polysaccharide and application thereof to preparation of whitening and spot-fading cosmetics

A technology of ginger grass and polysaccharide, applied in the field of chemistry, can solve problems such as activity reports without tyrosinase inhibitory effect, and achieve high inhibitory effect and improve effect.

Active Publication Date: 2020-01-21
浙江宝韵生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are very few studies on the polysaccharides in the whole herb of Gingergrass, and there is no report on its inhibitory effect on tyrosinase

Method used

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  • Micromeria biflora polysaccharide and application thereof to preparation of whitening and spot-fading cosmetics
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  • Micromeria biflora polysaccharide and application thereof to preparation of whitening and spot-fading cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: the extraction of ginger grass polysaccharide

[0021] Take the washed and dried whole ginger herb, crush it, pass through a 40-mesh sieve, add deionized water according to the ratio of material to liquid 1kg:20L, soak and extract at room temperature for 48 hours, collect the extract, filter, and carry out according to the ratio of 1kg whole herb to 1L concentrated solution Concentrate under reduced pressure, centrifuge at 4000r / min for 10min, ethanolize the supernatant with 4 times the volume of absolute ethanol for 12 hours, redissolve the precipitate in deionized water to the original volume of the supernatant, repeat with 4 times the volume of absolute ethanol Alcohol analysis once, the precipitate was alternately washed 3 times with ethanol and acetone according to 1g of precipitate corresponding to 50mL solvent, evaporated the solvent, redissolved in deionized water, deproteinized by Sevag method, and freeze-dried to obtain the product.

[0022] As d...

Embodiment 2

[0023] Embodiment 2: the extraction of ginger grass polysaccharide

[0024] Take the washed and dried whole ginger herb, crush it, pass through a 40-mesh sieve, add deionized water according to the ratio of material to liquid 1kg:15L, soak and extract at room temperature for 60h, collect the extract, filter, and carry out according to 1kg whole herb corresponds to 1L concentrated solution Concentrate under reduced pressure, centrifuge at 4000r / min for 10min, ethanolize the supernatant with 4 times the volume of absolute ethanol for 12 hours, redissolve the precipitate in deionized water to the original volume of the supernatant, repeat with 4 times the volume of absolute ethanol Alcohol analysis once, the precipitate was alternately washed 3 times with ethanol and acetone according to 1g of precipitate corresponding to 50mL solvent, evaporated the solvent, redissolved in deionized water, deproteinized by Sevag method, and freeze-dried to obtain the product.

[0025] As determi...

Embodiment 3

[0026] Embodiment 3: the extraction of ginger grass polysaccharide

[0027] Take the washed and dried whole ginger herb, crush it, pass through a 40-mesh sieve, add deionized water according to the ratio of material to liquid 1kg:25L, soak and extract at room temperature for 36h, collect the extract, filter, and proceed according to the ratio of 1kg whole herb to 1L concentrated solution Concentrate under reduced pressure, centrifuge at 4000r / min for 10min, ethanolize the supernatant with 4 times the volume of absolute ethanol for 12 hours, redissolve the precipitate in deionized water to the original volume of the supernatant, repeat with 4 times the volume of absolute ethanol Alcohol analysis once, the precipitate was alternately washed 3 times with ethanol and acetone according to 1g of precipitate corresponding to 50mL solvent, evaporated the solvent, redissolved in deionized water, deproteinized by Sevag method, and freeze-dried to obtain the product.

[0028] As determin...

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Abstract

The invention discloses Micromeria biflora polysaccharide and application thereof to preparation of whitening and spot-fading cosmetics. The polysaccharide is prepared by a method including crushing cleaned and dried Micromeria biflora whole herb, sieving, adding deionized water, performing soaking and extraction at normal temperature, collecting extract liquid, performing filtration, performing vacuum concentration, performing centrifugation to collect supernate, carrying out alcohol precipitation by using 4 times of volume of absolute ethyl alcohol, redissolving precipitate by using deionized water until the volume reaches the volume of the original supernate, repeatedly carrying out alcohol precipitation once by using 4 times of volume of absolute ethyl alcohol, alternately washing theprecipitate by using ethanol and acetone in sequence, volatilizing the solvents, redissolving the precipitate in the deionized water, deproteinizing by using a Sevag method, and freeze-drying the product. The polysaccharide provided by the invention has a relatively high inhibition effect on tyrosinase and has a prospect of being used for preparing whitening and spot-fading cosmetics.

Description

technical field [0001] The invention belongs to the field of chemistry, and in particular relates to a ginger grass polysaccharide and its application for preparing whitening and lightening cosmetics. Background technique [0002] Tyrosinase inhibitors are currently recognized as whitening additives for whitening cosmetics, such as kojic acid, a whitening and blemish-relieving ingredient. Therefore, strengthening the development of tyrosinase inhibitors can enrich the types of whitening and lightening additives in cosmetics. [0003] Plant polysaccharides are a gift from nature to humans. Many plant polysaccharides have biological activities, such as immune regulation, anti-tumor, hypoglycemic, hypolipidemic, anti-radiation, antibacterial, antiviral, and liver protection. Therefore, plant polysaccharides have long been widely used in the fields of public life such as medicine and catering. The application of plant polysaccharides in cosmetics has the advantage of being saf...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K8/73A61Q19/02
CPCA61K8/73A61Q19/02C08B37/0003
Inventor 陈敏
Owner 浙江宝韵生物科技有限公司
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