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Preparation method of clam oligopeptides

A clam and oligopeptide technology, which is applied in the field of preparation of clam oligopeptides, can solve problems such as unfavorable operation and increased viscosity, and achieve the effect of improving biological activity and extraction rate

Active Publication Date: 2020-01-21
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the good solvent properties of ionic liquids, ionic liquids are also used for peptide extraction research, but the viscosity of ionic liquids increases at low temperatures, which is very unfavorable for operation.
However, there is no research on the application of this binary system to peptide extraction.

Method used

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  • Preparation method of clam oligopeptides

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Effect test

Embodiment 1

[0029] A preparation method of clam oligopeptides, comprising the following steps:

[0030] (1) Wash the fresh clams, remove the shells, take the viscera, put them in water, stir and soak to remove impurities, then adjust the viscera of meretrix meretrix to neutrality, and then homogenate to prepare clam homogenate;

[0031] (2) The homogenate of meretrix meretrix and the compound solvent are uniformly mixed according to the material-to-liquid ratio of 1:2 to obtain the soaking liquid, and the compound solvent used is prepared by mixing ionic liquid EMIMBF4 with γ-butyrolactone at a volume ratio of 1:1;

[0032] (3) Reduce the temperature of the soaking liquid to -50°C, and pass pulse electric field treatment into the soaking liquid. The solution was kept at -50°C for 7 hours, and then rose to room temperature 23±2°C for 10 minutes;

[0033] (4) 0°C centrifugation step (3) treated soaking liquid 3 times, rotating speed 10000r / min, recovering the supernatant separated each tim...

Embodiment 2

[0038] A preparation method of clam oligopeptides, comprising the following steps:

[0039] (1) Wash the fresh clams, remove the shells, take the viscera, put them in water, stir and soak to remove impurities, then adjust the viscera of meretrix meretrix to neutrality, and then homogenate to prepare clam homogenate;

[0040] (2) The clam homogenate and the compound solvent are uniformly mixed at a material-to-liquid ratio of 1:4 to obtain the soaking liquid, and the compound solvent used is prepared by mixing ionic liquid EMIMBF4 with γ-butyrolactone at a volume ratio of 1:1;

[0041] (3) Reduce the temperature of the soaking solution to -55°C, and pass pulse electric field treatment into the soaking solution. The pulse electric field treatment method is: the number of pulses is 200 times, the electric field strength is 18kV / cm, the pulse width is 60μs, and the pulse frequency is 1000Hz. The solution was kept at -55°C for 8 hours, and then rose to room temperature for 20 minut...

Embodiment 3

[0047] A preparation method of clam oligopeptides, comprising the following steps:

[0048] (1) Wash the fresh clams, remove the shells, take the viscera, put them in water, stir and soak to remove impurities, then adjust the viscera of meretrix meretrix to neutrality, and then homogenate to prepare clam homogenate;

[0049] (2) The homogenate of meretrix meretrix and the compound solvent are uniformly mixed according to the material-to-liquid ratio of 1:3 to obtain the soaking liquid, and the compound solvent used is prepared by mixing ionic liquid EMIMBF4 with γ-butyrolactone at a volume ratio of 1:1;

[0050] (3) Reduce the temperature of the soaking solution to -60°C, and pass pulse electric field treatment into the soaking solution. The pulse electric field treatment method is: the number of pulses is 260 times, the electric field strength is 20kV / cm, the pulse width is 70μs, and the pulse frequency is 1100Hz. The solution was kept at -60°C for 7 hours, and then rose to r...

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Abstract

The invention relates to the technical field of polypeptide extraction from clams. In order to further improve the extraction rate of the clam oligopeptides, the invention provides an extraction method of the clam oligopeptides. A binary system obtained by compounding ionic liquid EMIMBF4 and gamma-butyrolactone according to a volume ratio of 1:1 is applied to the preparation of the clam oligopeptides; a pulse electric field is applied at a low temperature for decomposing internal organ tissues of the clams; then, enzymolysis, separation and purification are performed under an optimized enzymolysis condition; and the clam oligopeptides are obtained. The preparation method of the clam oligopeptides has the advantages that a compounding solvent prepared by mixing the ionic liquid EMIMBF4 andthe gamma-butyrolactone according to a ratio of 1:1 is added; low-temperature cooperative pulse electric field treatment is performed; then, enzymolysis is performed by using alkaline proteases; theextraction rate of the obtained clam oligopeptides is improved; the bioactivity is improved to a certain degree; and the degreasing process of 0.1 mol / L sodium hydroxide solution is omitted.

Description

technical field [0001] The invention relates to the technical field of extracting polypeptides from clams, in particular to a preparation method of clams oligopeptides. Background technique [0002] Meretrix meretrix is ​​mainly produced in the coastal area. It has high economic value, rich nutrition, delicious meat, and high therapeutic and medicinal value. Li Shizhen's "Compendium of Materia Medica" said that clams can cure "sores, boils, swollen toxins, eliminate accumulations, and relieve alcohol poisoning". It has obvious inhibitory effect, and also has obvious curative effect on diseases such as asthma, chronic bronchitis, goiter, and lymphatic tuberculosis. In the previous Chinese patent application CN201410624999.7, the inventor team of the present application provided a method for preparing clam oligopeptides. After the viscera of clams were homogenized, sodium hydroxide solution was degreased, alkaline protease was enzymatically hydrolyzed, and then chromatographi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C12P21/06C07K1/34C07K1/16C07K1/24C07K1/36
CPCC07K7/06C12P21/06
Inventor 杨最素唐云平余方苗黄芳芳陈艳
Owner ZHEJIANG OCEAN UNIV
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