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A method for synchronously monitoring chemical signals and electrical signals of nerve cell network

A technology of nerve cells and electrical signals, applied in the direction of electrochemical variables of materials, measuring devices, scientific instruments, etc., to achieve the effects of short service life, low yield, high sensitivity and time resolution

Active Publication Date: 2022-02-08
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on synchronous monitoring of electrical and chemical signals

Method used

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  • A method for synchronously monitoring chemical signals and electrical signals of nerve cell network
  • A method for synchronously monitoring chemical signals and electrical signals of nerve cell network
  • A method for synchronously monitoring chemical signals and electrical signals of nerve cell network

Examples

Experimental program
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Embodiment 1

[0033] This embodiment employs an array of 8 × 8 microelectrode ultra-thin thickness of 180μm, model 60ThinMEA200 / 30iR-ITO, such as layout figure 1 Indicated; figure 1 Electrode middle position shown, the REF is the reference electrode, is looped around the pin, wherein the first electrode number is the number of columns, the number of second bit lines; wherein the individual electrode diameter 10 m, a pitch of 30 m, the electrode resistance 250 ~ 400kΩ, the electrode material is of TiN; microelectrode array surface provided with reservoirs, an inner diameter of 24mm, a height of 12mm.

[0034] Detection apparatus according to the present embodiment as those of the figure 2 As shown in the microelectrode array 1 is mounted on the stage of total internal reflection fluorescence microscopy, microelectrode array 1 is positioned directly above the total internal reflection of the objective lens 3; the collected micro-electrode array 1 via the electric signal controlled microelectrod...

Embodiment 2

[0044] The present embodiment employs, as in Example detecting means and a micro-electrode array in Example 1, the same type; specific steps above-described micro-electrode array with the neural network to monitor the chemical and electrical signals with a cell total internal reflection fluorescence microscopy means of as follows:

[0045] (1) of the microelectrode array pretreatment: first microelectrode array rinsed in ethanol for 20 minutes, then air dried; With 75% ethanol for disinfection, naturally dried after irradiated with an ultraviolet lamp 6 hours; microelectrode fluid reservoir added 0.1mg / ml poly-lysine solution at 37 ℃, 5% carbon dioxide atmosphere in the stand for 1 hour; then thoroughly washed with distilled water microelectrode array; Finally, chemical modification, the laminin liquid sump disposed, allowed to stand overnight at 4 ℃, the electrode array used in cell culture;

[0046] (2) Construction of Neural Network Model microelectrode array surface: a conce...

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Abstract

The invention discloses a method for synchronously monitoring chemical signals and electrical signals of nerve cell networks. Firstly, the nerve cell network is cultivated in situ on a pretreated microelectrode array; then, the neuron vesicles are dyed, and then buffer solution is added ; Observing the nerve cell network with a total internal reflection fluorescence microscope, connecting the shooting camera and the controller of the microelectrode array with the pulse signal generator; performing external stimulation on the nerve cell network, and simultaneously turning on the pulse signal generator, shooting the camera and the microelectrode array The controller collects the fluorescent signal and the electrical signal synchronously, respectively. The synchronous monitoring method of the present invention has low background signal, high signal-to-noise ratio, and has space-time resolution responsiveness, which can meet the monitoring requirements of nerve cell networks; the method has stable signals, high sensitivity, and can realize imaging measurement for various nerve cells and networks, and High-throughput analysis can be achieved.

Description

Technical field [0001] The present invention relates to a method of detecting nerve cell, particularly to a neural network chemical cell synchronization signals and electrical monitoring method. Background technique [0002] Explore the structure of the information processing mechanisms of the nervous system, revealing the essence of higher nervous activity have a more far-reaching 8111. To achieve all the advanced features are inseparable from the nervous system to produce a signal, transmission, processing and storage. Neurons, also known as nerve cells, is the basic unit constituting the structure and function of the nervous system, which interacts connected to form an extremely complex network system, by electrical and chemical signaling behavior condition of the organism. Neuron cluster research information delivery mechanism to save lives closer to the essence of great scientific significance and practical value, the difficulty is very large. [0003] Signal transmission ne...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64G01N27/327G01N27/48
CPCG01N21/6428G01N27/48G01N27/3277
Inventor 徐琮辉赵微崔美荣徐静娟陈洪渊
Owner NANJING UNIV
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