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Regulating effect of zuojin wan (ZJW) on ROCK/PTEN/PI3K signal pathway of human gastric cancer drug-resistant cells and application of ZJW

A drug-resistant technology for gastric cancer cells and gastric cancer, applied in the field of biomedicine, can solve the problems of patients' quality of life and poor prognosis

Inactive Publication Date: 2020-01-14
SHANGHAI PUTUO DISTRICT CENT HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Gastric cancer is a common malignant tumor of the digestive system in my country, with high morbidity and mortality. Once the surrounding invasion or metastasis is found, surgical treatment is often palliative, and the quality of life and prognosis of patients are very poor.

Method used

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  • Regulating effect of zuojin wan (ZJW) on ROCK/PTEN/PI3K signal pathway of human gastric cancer drug-resistant cells and application of ZJW
  • Regulating effect of zuojin wan (ZJW) on ROCK/PTEN/PI3K signal pathway of human gastric cancer drug-resistant cells and application of ZJW

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Effect of Zuojin Wan on the expression of proteins related to ROCK / PTEN and PI3K pathways

[0020] ROCK inhibitor Y27623, PI3K inhibitor LY294002, Anti-PI3K (#3011), etc. were purchased from CST Corporation, USA.

[0021] (1) After ZJW (50μg / ml) treated SGC7901 / DDP cells for 0h, 1h, 3h, 6h, 12h, 24h, and 48h, the total protein was extracted, and Western blot was used to detect PI3K, p-PI3K, AKT, p-AKT, PTEN , p-PTEN, ROCK, cleaved ROCK expression.

[0022] Experimental results: The expression of PI3K did not change, but the expression of p-PI3K decreased significantly with the extension of ZJW treatment time to 24h and 48h, indicating that ZJW can inhibit PI3K in a time-dependent manner; AKT and p-AKT both increased with ZJW The treatment time prolongs to 24h and 48h and decreases, indicating that ZJW can inhibit AKT signal in a time-dependent manner; the expression of PTEN has no obvious change, while p-PTEN gradually increases with the prolongation of ZJW t...

Embodiment 2

[0025] Example 2 Inhibition of ROCK and PI3K signaling on ZJW-induced apoptosis and cycle of gastric cancer drug-resistant cells

[0026] ROCK inhibitor Y27623 and PI3K inhibitor LY294002 were used to inhibit ROCK and PI3K, respectively, to observe the effect of ZJW on apoptosis induction of gastric cancer drug-resistant cells. SGC7901 / DDP cells were treated with ZJW, Y27623, ZJW+Y27623, LY294002, ZJW+LY294002 respectively.

[0027] Experimental results: Compared with the control group, ZJW can induce apoptosis of SGC7901 / DDP cells; Y27623 alone has no significant change; ZJW+Y27623 combined can induce apoptosis of SGC7901 / DDP cells significantly lower than that of ZJW alone, indicating the activation of ROCK signal It is an important factor for ZJW-induced apoptosis; LY294002 alone significantly increases the apoptosis of SGC7901 / DDP cells, indicating that PI3K signaling is an inhibitory signal of apoptosis; the combined application of ZJW+LY294002 has a greater effect on the...

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Abstract

The invention discloses a regulating effect of zuojin wan (ZJW) on a ROCK / PTEN / PI3K signal pathway of human gastric cancer drug-resistant cells and application of ZJW. The influence of ROCK and PI3K signals on ZJW inducing apoptosis and a cycle of the gastric cancer drug-resistant cells is inhibited, and the results show that ZJW can induce apoptosis of SGC7901 / DDP cells. No significant change occurs when Y27623 is applied alone. Apoptosis induction of ZJW and Y27623 combination to the SGC7901 / DDP cells is significantly lower than that of ZJW alone, and it is indicated that activation of the ROCK signals is an important factor for ZJW to induce apoptosis. LY294002 applied alone significantly increases apoptosis of the SGC7901 / DDP cells, and it is indicated that the PI3K signals are inhibitory signals of apoptosis. The induction effect of apoptosis of the SGC7901 / DDP cells by combined use of ZJW and LY294002 is further increased rather than ZJW alone, and it is indicated that the two treatment methods have a synergistic effect.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the regulatory effect of Zuojin Wan on the ROCK / PTEN / PI3K signaling pathway of human gastric cancer drug-resistant cells and its application. Background technique [0002] Gastric cancer is a common malignant tumor of the digestive system in my country, with high morbidity and mortality. Once the surrounding invasion or metastasis is found, surgical treatment is often palliative, and the quality of life and prognosis of patients are very poor. Abnormal activity of PI3K-AKt pathway can not only lead to malignant transformation and malignant proliferation of cells, but also is related to migration, adhesion, tumor angiogenesis, and degradation of extracellular matrix in the process of tumor cell invasion and metastasis. Therefore, the prevention and treatment of tumors by inhibiting the activity of PI3K-AKt pathway has become a research hotspot. Zuojin Wan (ZJW) comes from Zhu...

Claims

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Application Information

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IPC IPC(8): A61K45/06A61K31/4375A61K31/519A61K31/5377A61P35/00
CPCA61K31/4375A61K31/519A61K31/5377A61K45/06A61P35/00A61K2300/00
Inventor 汤庆丰孙梦瑶孙健倪振华袁玉霞
Owner SHANGHAI PUTUO DISTRICT CENT HOSPITAL
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