Construction and evaluation method for experimental animal model of depression with gastrointestinal motility disorder

A technology of gastrointestinal motility disorder and experimental animals, which is applied in animal husbandry and other fields, and can solve problems associated with gastrointestinal diseases

Inactive Publication Date: 2020-01-14
SHANXI UNIV
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Problems solved by technology

[0006] Aiming at the problem that the existing drug treatment of depression is often accompanied by gastrointestinal diseases, the present invention provides a method for the construction and evaluation of an experimental animal model of depression with gastrointestinal motility disorder

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  • Construction and evaluation method for experimental animal model of depression with gastrointestinal motility disorder
  • Construction and evaluation method for experimental animal model of depression with gastrointestinal motility disorder
  • Construction and evaluation method for experimental animal model of depression with gastrointestinal motility disorder

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Embodiment Construction

[0033] A method for constructing an experimental animal model of depression with gastrointestinal motility disorder:

[0034] Male SD rats are used as experimental objects, and the experimental animals are raised in an environment with a temperature of 20-25°C and a humidity of 55±22%, with a natural circadian rhythm. They were randomly divided into blank group and model group, with 10 animals in each group.

[0035] A CUMS model was prepared. The model group was modeled by CUMS, and 9 different stress modes were randomly given: water-free for 24h, fasting for 24h, day and night reversal for 24h, restraint for 3h, 36V foot electric shock for 2min, 4℃ ice water swimming for 5min, 45℃ Thermal stimulation for 10 minutes, tail-clamping for 2 minutes, and 60W ultrasonic stimulation for 3 hours. One stimulus was given randomly every day for 21 consecutive days. The rats in the blank group were kept in normal groups. After the experiment, behavioral indicators were used to test whether t...

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Abstract

The invention belongs to the technical field of construction and evaluation methods for experimental animal models, and particularly relates to a construction and evaluation method for an experimentalanimal model of depression with gastrointestinal motility disorder. A CUMS (chronic unpredictable mild stress) model can be used to simulate the depression, intraperitoneal injection of L-arginine can simulate the gastrointestinal motility disorder, and through combination of the two, the experimental animal model of the depression with the gastrointestinal motility disorder is obtained. A behavioral test is an effective detection indicator for evaluating the depression, and the gastric emptying rate and the small intestinal advancing rate are effective indicators for evaluating the gastrointestinal motility disorder. Obtained behavioral data and gastrointestinal motility disorder detection indicator data are processed through statistical inspection, experimental animals have differencesbefore and after modeling, the differences reflect, to a certain degree, whether construction of the experimental animal model is successful or not, and accordingly, the experimental animal model forresearch on the depression with the gastrointestinal motility disorder is evaluated in a targeted way. It can be seen from comparison that the evaluation method can more fully reflect the performancecharacteristics of clinical diseases.

Description

Technical field [0001] The invention belongs to the technical field of experimental animal model construction and evaluation methods, and specifically relates to a method for construction and evaluation of an experimental animal model of depression with gastrointestinal motility disorder. Background technique [0002] Depression, also known as depressive disorder, is a common mental illness. It is mainly characterized by significant and lasting depression, slow thinking, cognitive impairment, and decreased volition. In addition to the "three low symptoms", it also produces one Series of other diseases, such as gastrointestinal diseases, anxiety disorders, etc. It has been clinically found that patients with depression often have symptoms of digestive system discomfort, and digestive system disorders are usually related to affective disorders. The incidence of gastrointestinal diseases is high, and often accompanied by some bad moods. Among them, depression and anxiety are mostly,...

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Application Information

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IPC IPC(8): A01K67/02
CPCA01K67/02A01K2227/105A01K2267/03
Inventor 田俊生赵映霞向欢秦雪梅张丽增
Owner SHANXI UNIV
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