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Method for detecting 11 pigments in tobacco reconstituted tobacco

A technology of reconstituted tobacco leaves and detection methods, which is applied in the field of detection, can solve the problems of false positive quantification, inaccuracy, and interference of multiple mixed pigment analysis, and achieve fast analysis speed, avoid false positives, and achieve the effect of more types of pigment screening

Active Publication Date: 2020-01-10
SHANGHAI TOBACCO GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, liquid chromatography has certain limitations. The complex matrix of the sample will interfere with the analysis of various mixed pigments. The UV detector only relies on the retention time for qualitative analysis, which is prone to false positive or inaccurate quantitative results.

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  • Method for detecting 11 pigments in tobacco reconstituted tobacco
  • Method for detecting 11 pigments in tobacco reconstituted tobacco
  • Method for detecting 11 pigments in tobacco reconstituted tobacco

Examples

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Embodiment Construction

[0067] The implementation of the present invention will be illustrated by specific specific examples below, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.

[0068] Before further describing the specific embodiments of the present invention, it should be understood that the protection scope of the present invention is not limited to the following specific specific embodiments; It is not intended to limit the protection scope of the present invention. The test methods for which specific conditions are not indicated in the following examples are usually in accordance with conventional conditions, or in accordance with the conditions suggested by each manufacturer.

[0069] When the examples give numerical ranges, it should be understood that, unless otherwise stated in the present invention, the two endpoints of each numerical range and any value between the two endpoints ...

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Abstract

The invention discloses a method for detecting 11 pigments in tobacco reconstituted tobacco. The method comprises the following steps of: 1) establishing high-resolution mass spectrum library information of 11 pigments by adopting double ternary high performance liquid chromatography and a high-resolution mass spectrum, the high-resolution mass spectrum database information comprising pigment names, retention time, [M+H]+ and [M-2H]2- ion accurate mass numbers, and characteristic fragment ion accurate mass numbers; and 2) performing pretreatment of to-be-detected tobacco reconstituted tobacco:extracting the to-be-detected tobacco reconstituted tobacco with a solvent to obtain to-be-detected liquid. The method has the advantages of the simple operation, the rapid detection and the high sensitivity, and can be applied to daily detection of reconstituted tobacco in cigarettes.

Description

technical field [0001] The invention relates to a detection method, in particular to a detection method of 11 pigments in reconstituted tobacco leaves. Background technique [0002] Pigments, also known as colorants, can be divided into two categories: natural pigments and synthetic pigments according to their sources. Natural pigments are extracted from animals and plants, have poor stability and are expensive. Artificial pigments refer to organic pigments prepared by artificial chemical synthesis methods, mainly made of aniline dyes separated from coal tar. According to structure, artificial pigments can be classified into azo, xanthracene and diphenylmethane, etc.; according to solubility, they can be divided into fat-soluble and water-soluble pigments. A large number of research reports have shown that almost all artificial pigments not only cannot provide nutrition for the human body, but also some artificial pigments will endanger human health, lead to decreased fert...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06G01N30/72
CPCG01N30/88G01N30/06G01N30/72G01N2030/884G01N2030/062G01N2030/045
Inventor 戚大伟周妍罗辰王戈王超费婷吴达刘百战
Owner SHANGHAI TOBACCO GRP CO LTD
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