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Method and kit for simultaneously detecting four inflammatory markers

A marker and kit technology, applied in the field of biomedicine, can solve the problem that the kit cannot detect accurately and efficiently at the same time, and achieve the effects of good batch-to-batch difference, good repeatability and high precision

Pending Publication Date: 2020-01-03
湖北新纵科病毒疾病工程技术有限公司
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The problem solved by the present invention is that the kits in the prior art cannot accurately and efficiently detect the four markers of CRP, PCT, IL-6 and SAA at the same time

Method used

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Embodiment Construction

[0024] In order to make the above objects, features and advantages of the present invention more comprehensible, specific embodiments of the present invention will be described in detail below.

[0025] An embodiment of the present invention provides a kit for simultaneously detecting four inflammatory markers, including microsphere reagents and biotinylated antibodies;

[0026] Among them, the microsphere reagent includes four kinds of coded microspheres, and the four coded microspheres are respectively coupled with specific antibodies for four inflammatory markers, and the four inflammatory markers are PCT, IL-6, CRP and SAA;

[0027] Biotinylated antibodies include detection antibodies for four inflammatory markers, and the detection antibodies are labeled with fluorescein.

[0028] The kit for simultaneously detecting four inflammatory markers provided by the embodiment of the present invention contains two or more fluorescent materials inside the blank microspheres, and t...

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Abstract

The invention provides a method and a kit for simultaneously detecting four inflammatory markers. The kit for simultaneously detecting four inflammatory markers comprises: a microsphere reagent and abiotinylation antibody; wherein the microsphere reagent comprises four types of encoded microspheres, the four types of encoded microspheres respectively correspond to specific antibodies coupled to the four inflammatory markers, and the four types of inflammatory markers are PCT, IL-6, CRP and SAA; and the biotinylation antibody comprises detection antibodies for the four inflammatory markers, and the detection antibodies are labeled with fluorescein. The kit provided by the invention can simultaneously detect the concentrations of the four indicators of PCT, IL-6, CRP and SAA in the serum, thereby realizing the quantitative detection of the indicators, having high efficiency and reducing the cost, furthermore, the detection concentration range is wide, the required sample volume is small, and the variable coefficient of intra-batch and inter-batch experiments is small.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for simultaneously detecting four inflammatory markers and a kit thereof. Background technique [0002] Inflammation is a defense response of living tissue with vascular system to damage factors. It is a very common and important basic pathological process, mainly divided into bacterial infection and viral infection. Infection is a common clinical disease. How to identify, diagnose and evaluate the degree of infection early is the basic link in clinical treatment. Studies have shown that C-reactive protein (CRP), procalcitonin (PCT), interleukin-6 (IL-6), and serum amyloid A (SAA) are important diagnostic aids in the early stages of infection and the development of inflammation significance. [0003] In the prior art, the diagnosis is generally made by detecting CRP, PCT, IL-6 or SAA alone, and the accuracy of the detection results is low, and the detection time is...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/58G01N33/543
CPCG01N33/6893G01N33/6869G01N33/582G01N33/585G01N33/54326G01N2333/5412G01N2333/4737
Inventor 王华林张涛谢进超王万朝韩迪
Owner 湖北新纵科病毒疾病工程技术有限公司
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