Lactobacillus paracasei for degrading grease and application thereof

A technology of lactobacillus and paracheese, applied in the field of lactic acid bacteria, can solve the problems of difficult to resist the taste and flavor of high-fat food

Active Publication Date: 2020-01-03
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the ways to reduce fat intake are mainly to change food cooking methods, reduce intake or use fat substitutes, etc. Although these methods can reduce fat intake to a certain extent, it is still difficult for people to resist the temptation of high-fat foods taste and flavor

Method used

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  • Lactobacillus paracasei for degrading grease and application thereof
  • Lactobacillus paracasei for degrading grease and application thereof
  • Lactobacillus paracasei for degrading grease and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] 1. Strain screening

[0074] (1) Domestication of strains

[0075] Under aseptic conditions, use a pipette gun to draw appropriate amount of samples of five kinds of distiller's grains, which are marked as "S1, S2, S3, S4, S5" respectively, and inoculate these five kinds of distiller's grains samples into MRS and M17 medium, respectively. They were cultured in a constant temperature incubator at 37°C and 42°C, with a total of 20 parallel samples, and then the initially cultured strains were streaked and domesticated.

[0076] After 48 hours of acclimatization culture in MRS and M17 medium, the strain culture results in distiller's grains were as follows: figure 1 , as shown in Table 2, the colonies in only 16 petri dishes are more in line with the colony morphological characteristics of lactic acid bacteria in appearance, and the primary screening culture is further carried out.

[0077] Table 2 Strain acclimation results

[0078]

[0079]

[0080] (2) Prelimin...

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PUM

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Abstract

The invention discloses lactobacillus paracasei F3 for degrading grease. The preservation number of the lactobacillus paracasei F3 is CGMCC No.18355. the lactobacillus paracasei F3 can degrade blend oil, peanut oil and sesame oil, the degradation effect on the sesame oil is the best, and the degradation rate is 39.38%. The strain can detect obvious lipase activity, and the enzyme activity is as high as 11.08 U/mL. Meanwhile, the lactobacillus paracasei F3 can degrade various fatty acids in the sesame oil, especially linoleic acid, oleic acid, trans-linoleic acid, palmitic acid, linolenic acidand other fatty acids, and the total degradation rate of the fatty acids in the sesame oil can reach 6.71 mg/mL. The lactobacillus paracasei F3 shows excellent acid tolerance and bile salt tolerance in tolerance experiments of artificial gastric juice, artificial intestinal juice and bile salt, can tolerate gastric juice and intestinal juice of a human body, can be used as probiotics with excellent performance, survives in intestinal tracts and plays a role. The lactobacillus paracasei F3 can be used as a food additive or directly prepared into foods, health care products and the like.

Description

technical field [0001] The invention belongs to the technical field of lactic acid bacteria, and relates to a strain of Lactobacillus paracasei which degrades oil and its application. Background technique [0002] Dietary fat is an important source of energy for the human body and plays an important role in maintaining the energy balance of the human body. With the development of the economy, adequate food supply and other factors, dietary fat intake has increased significantly. Excessive dietary fat intake can promote the occurrence of chronic diseases such as obesity, coronary heart disease, diabetes and tumors. At present, the ways to reduce fat intake are mainly to change food cooking methods, reduce intake or use fat substitutes, etc. Although these methods can reduce fat intake to a certain extent, it is still difficult for people to resist the temptation of high-fat foods taste and flavor. [0003] After the fat is ingested, it mainly depends on the pancreatic lipas...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C19/06A23L13/40A23L33/135C12R1/225
CPCC12N1/20A23C19/062A23L13/45A23L33/135A23V2002/00C12R2001/225C12N1/205A23V2400/165A23V2200/3204
Inventor 关成冉陶志强王丽顾瑞霞陈大卫张臣臣黄玉军陈霞马文龙
Owner YANGZHOU UNIV
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