System for biomolecule sequencing through Raman spectroscopy

Abstract

The invention provides a system for biomolecule sequencing through Raman spectroscopy. The system comprises a nanopore device, a laser Raman microscope, a spectral measurement device and a data acquisition and analysis device. The nanopore device is located below the laser Raman microscope; the nanopore device mainly comprises a nanopore cavity, a nanopore, a grating and a metal layer; the nanopore is an opening in one end of the nanopore cavity, and the nanopore cavity is a reduced cavity and extends towards the nanopore; the grating surrounds the nanopore cavity; the metal layer is used fordecreasing the size of the nanopore to 1-100 nm; and when to-be-measured biomolecules pass through the nanopore, the laser Raman microscope emits lasers to the biomolecules to generate a Raman spectrum signal, the spectral measurement device measures data, and the data acquisition and analysis device analyzes and outputs a result. By using a surface enhanced Raman spectrum detection strategy, thesystem has the technical effect so that the sub-nano spatial resolution can be detected; and a vibration spectrum of molecular specific fingerprint information is provided, all base spectrum signals are not overlapped, and excellent discrimination and chemical sensitivity are achieved.

Description

technical field [0001] The invention belongs to the fields of gene sequencing and biomolecular sensing, and in particular relates to a system and a sequencing method for biomolecular sequencing using Raman spectroscopy. Background technique [0002] The measurement of genetic information has revolutionized the field of life sciences and medicine. Future precision medicine and personalized medicine require new sequencing technologies with lower cost, faster speed, higher precision and longer read length. [0003] The new generation of single-molecule real-time sequencing technology addresses the need for longer read length and faster speed; the recent rapid development of biological nanopore sequencing technology further addresses the need for lower cost. Biological nanopore sequencing technology does not need to prepare a large number of samples, and the sample preparation process does not need to consume biological and chemical reagents, which greatly reduces the cost of s...

AI Technical Summary

Problems solved by technology

The current solid-state nanopore technology mainly realizes sequencing by measuring the ion blocking current, but it faces many challenges: first, the translocation behavior of the DNA chain in the nanopore is not easy to control, and the direction of the base is uncontrolled, which has great Randomness, DNA moves too fast (0.1-1μs/bp); DNA binds non-specifically to the surface of the nanopore to form a secondary or tertiary structure, blocks the nanopore, and restricts the normal translocation of the DNA c

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