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Microbial agent and applications thereof

A microbial agent, plant technology, applied in the direction of application, biocide, animal repellent, etc., can solve the problems of high utilization cost, secondary pollution, etc., and achieve the effects of improving quality, improving soil, and promoting plant growth

Active Publication Date: 2019-12-31
JIANGSU GUOXIN UNION ENERGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, improvement measures such as irrigation and leaching, mulch salt control, and application of chemical modifiers can improve saline-alkali soil and promote plant growth, but the use cost is high and secondary pollution is likely to occur

Method used

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  • Microbial agent and applications thereof
  • Microbial agent and applications thereof
  • Microbial agent and applications thereof

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preparation example Construction

[0029] In the present invention, the preparation method of the microbial agent preferably comprises: after mixing the citric acid fermentation tail liquid, water, peptone and yeast powder, adjusting the pH to 5.5-6.5 with ammonia water, and then extinguishing at 115-125°C Bacteria for 15-25 minutes to obtain a mixed solution; inoculate the alkalophilic bacillus into the above mixed solution and cultivate to obtain a microbial agent.

[0030] In the present invention, the mass concentration of the ammonia water is preferably 20-30%, more preferably 25%. The pH value is preferably 5.5-6.5, more preferably 6.0. The present invention uses ammonia water to adjust the pH because other alkaline solutions such as sodium hydroxide and potassium hydroxide will bring potassium and sodium ions into the soil, increasing the salinity of the soil.

[0031] In the present invention, the sterilization temperature is preferably 115-125° C., more preferably 121° C.; the sterilization time is pr...

Embodiment 1

[0041] Microbial agent preparation method

[0042]Dilute 20 mL of the citric acid fermentation tail liquid with 980 mL of distilled water to 1000 mL, add 10 g of peptone to the above liquid at room temperature, add 5 g of yeast powder and stir until uniform, and adjust the pH value to 6.0 with 25% ammonia water. The above liquid was divided into 1000mL glass conical flasks so that the liquid volume in the bottle was 500mL, sealed with a sealing film, sterilized at 121°C for 20min, and the sterilized liquid was left at room temperature. Among them, the pH of the citric acid tail liquid is 3.35, the organic matter content is 200.55g / L, the humic acid is 2.35g / L, the total nitrogen is 106.10g / L, the total phosphorus is 2.82g / L and the total potassium is 16.85g / L.

[0043] The alkalophilic Bacillus wp-1 strain in the LB slant medium stored in the refrigerator at 4°C was inoculated into the sterilized LB liquid medium at pH 8.0, shaken at 180r / min for 48 hours at 30°C. Among them,...

Embodiment 2

[0046] The application of the microbial bacterial agent of embodiment 1 in the growth, quality, output and saline-alkali soil improvement of Lycium barbarum.

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Abstract

The invention provides a microbial agent and applications thereof, and relates to the technical field of biological agents. The microbial agent comprises citric acid fermentation tail liquid, peptone,yeast powder, bacillus alcaligenes and water; wherein the volume ratio of the citric acid fermentation tail liquid to the bacillus alcaligenes to the water to the peptone to the yeast powder is (15-25)mL: (2-3) mL:(975-985) mL:(8-12) g: (4-6) g; the content of organic matters in the citric acid fermentation tail liquid is 150-250g / L. The microbial agent provided by the invention not only has a remarkable effect of improving saline-alkali soil, but also can promote crop growth, increase crop yield and improve fruit quality.

Description

technical field [0001] The invention relates to the technical field of biological bacterial agents, in particular to a microbial bacterial agent and its application. Background technique [0002] As an important chemical raw material and food additive, citric acid is widely used in food, medicine, chemical and other industries. At present, China has nearly 100 citric acid production plants with a total annual production capacity of about 800,000 tons. It is the world's largest producer and exporter of citric acid. The citric acid tail liquid mainly contains starch, protein, carbohydrates, various organic acids, ammonia nitrogen and fat, etc., with a concentration of 20,000-30,000 mg / L. It belongs to high-concentration organic wastewater. If it is discharged directly, it will pollute the environment and destroy the soil (Wang Mingxing, Liao Changjian. Biological treatment technology for high-concentration organic wastewater [J]. Contemporary Chemical Industry, 2011, 40, (8) ...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01N63/02C09K17/14A01P21/00C09K101/00C09K109/00
CPCA01N63/00C09K17/14C09K2101/00C09K2109/00Y02A40/10
Inventor 张凤华王丹赵亚光孙福新
Owner JIANGSU GUOXIN UNION ENERGY CO LTD
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