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Method for modifying fruits, etc. and cultured cells thereof

A technology for cultivating cells and fruits, applied in plant cells, horticultural methods, plant genetic improvement, etc., can solve problems such as unclear action process, achieve increased repellent effect, increased sugar content or acidity, and increased disease resistance Effect

Inactive Publication Date: 2019-12-27
가부시키가이샤간덴세이사쿠쇼
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, in Patent Document 3, there is no record about the control effect of diseases and insect pests and the increase or decrease of the probable enzyme, and its action process is also unclear.

Method used

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  • Method for modifying fruits, etc. and cultured cells thereof
  • Method for modifying fruits, etc. and cultured cells thereof
  • Method for modifying fruits, etc. and cultured cells thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] (About electrical stimulation of grape cultured cells)

[0059] It will be explained that grape cultured cells can be improved by applying electric current stimulation to grape cultured cells.

[0060] figure 1 Shown is a flow chart of the method for improving grape cultured cells involved in this embodiment. Such as figure 1 As shown, an electrode and a counter electrode are inserted into an aggregate of cultured grape cells (S11), and then both electrodes of the cultured grape cells are brought into contact with the aggregate (S12). The electrodes are connected to the solar panel, and a voltage is applied between the electrodes by illuminating light (S13). The illumination light is adjusted so that the temperature of the aggregate of grape cultured cells is lower than 50° C. (S14). Then, it was confirmed whether the grape cultured cells were improved (increase of enzymes, improvement of disease resistance).

[0061] Specifically, as figure 2 As shown in the s...

Embodiment 2

[0087] (Regarding electrical stimulation of vines)

[0088] In this example, description will be given of the results of row current processing performed by embedding electrodes into the trunk of a vine, connecting a solar panel, and applying voltage to the electrodes.

[0089] image 3 Schematic flow chart of the grapevine improvement method in this embodiment. Such as image 3 As shown, the electrode and its counter electrode (S01) were inserted in different parts of the grape vine trunk. The two electrodes are brought into contact with the inner tissue of the vine trunk. By irradiating sunlight to the solar panel, a voltage is applied between the electrodes (S03). By adjusting the specifications of the solar panel, the temperature of the internal tissue is kept below 50°C (S04). Then, the sugar content, total acid, total phenol content, amino acid, and resveratrol content were actually measured from the juice of the grape fruit. In addition, actual measurements of the...

Embodiment 3

[0110] (Regarding electrical stimulation of vines)

[0111] In this example, an electrode is embedded in the trunk of a vine, a solar panel is connected to apply a voltage to the electrode, and the result of current processing is described.

[0112] Figure 11 A flowchart of the method for improving grapevines of the present embodiment is shown. Such as Figure 11 As shown, the electrode and its counter electrode (S01) were inserted in different parts of the grape tree trunk. Bring both electrodes into contact with the inner tissue of the vine trunk (S02). By irradiating sunlight to the solar panel, a voltage is applied between the electrodes (S03). By adjusting the specifications of the solar panel, the temperature of the internal tissue is kept below 50°C (S04). Then, the number of healthy grape pods was measured, and the incidence of disease was measured.

[0113] Same as embodiment 2, such as Figure 4 As shown in the experimental pattern diagram, two sets of electr...

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Abstract

Provided is a method whereby fruits, etc. are modified by electrically stimulating an orchard tree such as grape or a vegetable to thereby achieve modification effects of, for example, improving the disease resistance, enhancing the repellent effect, increasing the sugar content, decreasing the acidity, and increasing the enzyme content of the fruits, etc. An electrode and a counter electrode areinserted into different sites of an orchard tree or an annual herbal plant. Then, a voltage is impressed between the electrodes while keeping the both electrodes in contact with internal tissues. Thus, fruits, etc. are modified and, for example, the disease resistance thereof is improved. Examples of the orchard tree include not only fruit-bearing perennial arboreous plants but also rootstock plants for orchard trees such as banana, pineapple, papaya and passion fruit. According to this method, the expression amount of a gene capable of producing a disease-resistant protein can be increased within the plant, or the sugar content of fruits can be increased and the acidity thereof can be decreased. In the case where the orchard tree is a grape tree, in particular, the anthocyanin content andresveratrol content of grape fruits can be increased.

Description

technical field [0001] The present invention relates to a method for improving the cultured cells of fruits, vegetables and fruits, especially a method for improving disease resistance or repelling effect, increasing sugar content or reducing acidity, increasing enzymes, etc. improved method. Background technique [0002] In recent years, due to the problem of global warming due to the increase in carbon dioxide concentration, the protection of plants or forests and the necessity of these activations have received much attention. [0003] In this case, regarding the agricultural technology for promoting the activation and growth of plant biological functions, there is known a current-carrying body that connects the internal high potential inside the plant organism and the external low potential of the epidermis, and from the external epidermal side An agricultural technique that promotes the activation and growth of plant organisms by inserting plant organisms and increasin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/04A01H1/06A01H3/00A01H5/08A01H5/12A23L2/02A23L19/00A23L19/20C12N5/04
CPCA01G7/04A01H3/00A23B7/00A23L2/02A23L19/00A01G22/00C12N5/04Y02A40/924
Inventor 益村义幸森大辅铃木俊二三神允周
Owner 가부시키가이샤간덴세이사쿠쇼
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