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Ultra-high performance liquid chromatography detection method for flower of gentiana farreri balf.f.

An ultra-high performance liquid phase, gentian flower technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of unclear medicinal parts, lack of systematic research, confusion in classification, etc., and achieve enhanced resolution and sensitivity. , Improve the detection rate and the effect of accurate detection results

Inactive Publication Date: 2019-12-17
GANSU UNIV OF CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is still a lack of systematic research on this kind of Tibetan medicine, the varieties are complex, the medicinal parts are unclear, and the classification is chaotic.

Method used

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  • Ultra-high performance liquid chromatography detection method for flower of gentiana farreri balf.f.
  • Ultra-high performance liquid chromatography detection method for flower of gentiana farreri balf.f.
  • Ultra-high performance liquid chromatography detection method for flower of gentiana farreri balf.f.

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Experimental program
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Effect test

experiment example

[0041] 1. Test equipment and reagents:

[0042] (1) Test equipment (see Table 1):

[0043] Table 1 Test Instrument Information Sheet

[0044]

[0045] (2) Reagents:

[0046] Methanol (Thermo Fisher Scientific Co., Ltd., chromatographically pure), methanol (Tianjin Damao Chemical Reagent Factory, analytically pure), purified water (Watsons distilled water).

[0047] 2. Samples and reference substances:

[0048] (1) The sample is selected from the flower of Gentiana sylvestris, which was collected from Manrima Township, Maqu County, Gannan Prefecture, Gansu Province. It has been identified as Gentiana genus of Gentianaceae. Gentiana Farreri Balf. f.

[0049] (2) The reference substances were purchased from Shanghai Yuanye Biotechnology Co., Ltd., and their information is shown in Table 2.

[0050] Table 2 Reference substance information table

[0051]

[0052] 3. Preparation of the test solution: pick up the weeds, crush the gentian flower of the line to be tested, pas...

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Abstract

The invention discloses an ultra-high performance liquid chromatography detection method for the flower of gentiana farreri balf.f.. The method comprises the following steps: (1), preparing a test solution; and (2), carrying out ultra-high performance liquid chromatography analysis. According to the invention, the flower of gentiana farreri balf.f. can be applied and can be distinguished from similar varieties and adulterants of the flower of gentiana farreri balf.f.; the quality of the flower of gentiana farreri balf.f. can be evaluated and controlled comprehensively; the defect of one-sidedness caused by only comparing individual chemical components in the total components under the condition of determining the authenticity and the quality of the flower of gentiana farreri balf.f. can beovercome; and the detection method has high reproducibility and separation degree and is suitable for measurment of the index components of the flower of gentiana farreri balf.f. and establishment ofa fingerprint spectrum. The ultra-high performance liquid phase can accept the higher throughput and smaller sample amount and the better selectivity and better detection limit and quantification limit are provided.

Description

technical field [0001] The invention belongs to a quality control method of traditional Chinese medicinal materials, and in particular relates to an ultra-high-efficiency liquid phase detection method of gentian floss. Background technique [0002] Gentiana belongs to the order Gentianales Gentianaceae Gentianaceae, there are more than 400 species in the world, widely distributed around the world, mainly in the northern temperate zone and tropical alpine regions. There are 22 genera and more than 500 species in my country, of which 12 genera and more than 70 species are used for medicine. Gentiana is an annual or perennial herb. Among Gentianaceae, this genus has the most species of plants. Gentiana plants can be used for ornamental and medicinal purposes, and most of them are traditional Tibetan medicines. Tibetan medicine "Bangjian" is the general name of various medicinal plants of the genus Gentiana. It is a representative and commonly used Tibetan medicinal material. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 林丽晋玲郑贵森陈红刚高素芳赵凌豪刘立赵文龙王振恒崔治家
Owner GANSU UNIV OF CHINESE MEDICINE
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