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Construction method and application of electrochemical cell sensor for acetamiprid and imidacloprid joint toxicity evaluation

A technology of cell sensor and joint toxicity, which is applied in chemical instruments and methods, nanotechnology for materials and surface science, and electrochemical variables of materials, etc. It can solve the problems of inability to achieve uniform dispersion of nanoparticles and achieve good application prospects. Low cost and improved sensitivity

Active Publication Date: 2019-12-13
JIANGNAN UNIV
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Problems solved by technology

Despite these efforts, these methods still cannot achieve uniform dispersion of size-controllable nanoparticles on graphene

Method used

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  • Construction method and application of electrochemical cell sensor for acetamiprid and imidacloprid joint toxicity evaluation
  • Construction method and application of electrochemical cell sensor for acetamiprid and imidacloprid joint toxicity evaluation
  • Construction method and application of electrochemical cell sensor for acetamiprid and imidacloprid joint toxicity evaluation

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Embodiment Construction

[0023] The present invention will be further described below with reference to the accompanying drawings.

[0024] CHI660D electrochemical workstation (Shanghai Chenhua) used in the present invention; HITACHI S4800 field emission scanning electron microscope; JEM-2100 (HR) transmission electron microscope (Japan JEOL company); Nicolet iS50 FT-IR Fourier transform infrared spectrometer (Thermo Fisher of the United States) X-ray diffraction (XRD) measured by CuKα radiation on a D8 Advance (Bruker, Germany); Spectrofluorimeter (Varian Co., Ltd., USA).

[0025] The present invention adopts graphite, citric acid, histidine, sodium hydroxide, silver nitrate (AgNO 3 ), prepared phosphate buffered saline (PBS, 0.01 M, pH 7.40), Hep G2 cells, L-cysteine, 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride ) / (N-hydroxysuccinimide), folic acid, acetamiprid and imidacloprid standards.

[0026] A preparation of a cell sensor based on a three-dimensional composite material of size-c...

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Abstract

The invention relates to a construction method and application of an electrochemical cell sensor for acetamiprid and imidacloprid joint toxicity evaluation, and belongs to the technical field of celldetection. A size-controllable Ag / His-GQD / rGO three-dimensional composite material is prepared, after a glassy carbon electrode is modified with the composite material, L-cysteine is combined to the composite material through sulfydryl, activated folic acid is combined with L-cysteine through amido bonds, cells are fixed, determination is performed through an electrochemical impedance technology,and the electrochemical cell sensor is prepared. Through combination with a three-dimensional composite material, more binding sites can be provided, and the sensitivity of the sensor is improved. Combined with an electrochemical impedance technology, the constructed sensor can determine the Hep G2 cells quickly, and is relatively low in cost. The Hep G2 cell sensor can be used for evaluating thepesticide toxicity, and has a good application prospect in the aspect of pesticide joint toxicity evaluation.

Description

technical field [0001] The invention relates to a construction method and application of an electrochemical cell sensor for the combined toxicity evaluation of acetamiprid and imidacloprid, in particular to a size-controllable silver nanoparticle / histidine functionalized graphene quantum dot / reduction A preparation method of graphene oxide three-dimensional composite material and application thereof belong to the technical field of cell detection. Background technique [0002] With the development of agriculture, various pesticides have emerged, such as organophosphorus, carbamate, chlorinated nicotinyl, etc. Among them, chlorinated nicotinyl pesticides, as a new type of pesticides, are widely used in agricultural pest control and pest control. middle. It controls the nicotinic acetylcholine receptors present in the insect nervous system, blocks the normal conduction of the insect central nervous system, disrupts the potential balance of the synaptic membrane, induces paral...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/327C01B32/194B82Y30/00B22F9/16
CPCB22F9/16B22F2009/165B82Y30/00C01B32/194G01N27/308G01N27/3278
Inventor 李在均徐丹李瑞怡孙秀兰
Owner JIANGNAN UNIV
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