Construction method of in-vitro GBM invasion model based on biological three-dimensional printing

A technology of three-dimensional printing and construction methods, applied in the fields of biochemical equipment and methods, microorganisms, animal cells, etc., can solve the problems of lack, high cost of animal models, ethical issues, etc., and achieve the effect of maintaining stable mechanical properties

Inactive Publication Date: 2019-12-13
ZHEJIANG UNIV
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  • Application Information

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Problems solved by technology

Traditional two-dimensional cell culture models lack extracellular matrix and complex three-dimensional structures, and animal models are costly and ethically problematic. Therefore, new research methods are urgently needed to study the invasive growth of GBM

Method used

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  • Construction method of in-vitro GBM invasion model based on biological three-dimensional printing
  • Construction method of in-vitro GBM invasion model based on biological three-dimensional printing

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Embodiment Construction

[0025] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0026] like Figure 1-2 As shown, in this embodiment, the method for constructing an in vitro GBM invasion model based on biological three-dimensional printing includes the following steps:

[0027] 1) Preparation of bio-ink containing human brain glial cells; the solvent of the bio-ink is pure water, and the components of the bio-ink include hyaluronic acid, alginate and gelatin; the selection of the hyaluronic acid in the bio-ink The content is 0.02g / ml, the selected content of the alginate in the bio-ink is 0.02g / ml, and the selected content of the gelatin in the bio-ink is 0.010g / ml. The bio-ink also includes glucose as an invasion-inducing factor, and the amount of glucose added is 0.02 g / ml.

[0028] 2) The bio-ink is loaded into the feed system of the multi-nozzle micro-extrusion bio-3D printer, and the scaffold structure is printed according t...

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Abstract

The invention discloses a construction method of an in-vitro glioblastoma (GBM) invasion model based on biological three-dimensional printing, which belongs to the field of tissue engineering and biological three-dimensional (3D) printing. According to the method, hydrogel containing cells is used as biological ink, and an in-vitro GBM invasion model is constructed by using a biological three-dimensional printer. The model structure is formed by printing hydrogel carrying normal human brain glial cells, and is inoculated with glioma cell clusters. The model has important significance for researching invasive growth of GBM, and is expected to provide help for researching a treatment method of polymorphic glioblastoma.

Description

technical field [0001] The invention relates to a construction method of an in vitro GBM invasion model based on biological three-dimensional printing, which is used for constructing an in vitro GBM invasion model with high cell survival rate, and belongs to the field of tissue engineering technology and biological three-dimensional (3D) printing. Background technique [0002] Cancer seriously endangers the life and health of the people. Research by the Global Burden of Disease Organization shows that in 2016 alone, cancer caused as many as 8.93 million deaths worldwide. According to the 2017 Cancer Progress Report released by the American Association for Cancer Research (AACR), the number of cancer cases diagnosed and the number of cancer deaths worldwide each year The number of cancer cases is even more likely to exceed 24 million in 2035, which will cause a sharp increase in medical costs. In my country, there were 4.29 million new cases and 2.81 million deaths in 2015. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09B29C64/112B29C64/321B33Y10/00B33Y40/00
CPCB29C64/112B29C64/321B33Y10/00B33Y40/00C12N5/0693C12N2533/54C12N2533/74C12N2533/80
Inventor 马梁李雨亭吴钰桐张斌杨华勇
Owner ZHEJIANG UNIV
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