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Application of Eucalyptus polybractea essential oil and its composite microbial agent in preparation of vermifuge for echinoderms

A technology of compound bacterial agent and eucalyptus essential oil is applied in animal repellents, chemicals used for biological control, applications, etc., which can solve the problems of damage to aquatic animal health, unstable drug dosage, hidden dangers to consumer safety, etc. Reduce bacterial infection, save breeding costs, and use a wide range of effects

Active Publication Date: 2019-12-13
SHANDONG LONGCHANG ANIMAL HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in order to simply pursue immediate results, traditional medicines will cause excessive heavy metals and unstable drug dosages, which will destroy the stability of water quality, damage the health of aquatic animals and reduce their immunity. In severe cases, it will directly lead to death. , ultimately reducing production efficiency and bringing safety hazards to consumers

Method used

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  • Application of Eucalyptus polybractea essential oil and its composite microbial agent in preparation of vermifuge for echinoderms
  • Application of Eucalyptus polybractea essential oil and its composite microbial agent in preparation of vermifuge for echinoderms
  • Application of Eucalyptus polybractea essential oil and its composite microbial agent in preparation of vermifuge for echinoderms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Preparation of eucalyptus eucalyptus essential oil

[0030] The preparation process of Eucalyptus eucalyptus essential oil in the present invention is as follows figure 1 As shown, first put the leaves of Australian Eucalyptus eucalyptus into the washing tower for cleaning, put them in the dryer for drying, and then pulverize in the pulverizer and then enter the steam distillation kettle, the condenser and the oil-water layer to separate. The oil phase and the water phase. The water phase enters the ether extractor to separate the waste water, the liquid containing ether enters the ether recovery tower to recover the ether, and the recovered ether and oil phase liquid are added to the solid bed adsorption dryer to further remove the oil phase. Water, and finally the oil phase liquid is added to the vacuum distillation tower for rectification to remove the impurity oil, and then the multi- bud Eucalyptus essential oil is obtained.

[0031] The eucalyptus eucalyptus essenti...

Embodiment 2

[0041] The multi-bud eucalyptus eucalyptus essential oil compound microbial agent prepared in Example 1 was used for insect repellent experiments. The test site was selected in Linghai, Liaoning. Sea cucumbers were cultured at 2000 cubic meters, each cement pond was 25 cubic meters, and the breeding specifications were 1000-1500 heads / kg. . After the ulcer was found on the surface of the sea cucumber and the parasites were detected, 1 kg of eucalyptus eucalyptus essential oil compound microbial agent was mixed with 1 ton of feed, followed by normal feeding management, and fed for 4 consecutive days, 3 times a day. Four days later, check the sea cucumber's parasite condition again. Such as Figure 4 As shown, at the early stage of feeding, the sea cucumber body surface was ulcerated and ciliates were found in the wound; after 4 days of feeding, it was checked that the sea cucumber ulcerated wound was healed and the wound was free of ciliates. The deworming effect.

Embodiment 3

[0043] The multi-bud eucalyptus eucalyptus essential oil prepared by the present invention is subjected to an insect repellent experiment, and the test site is selected at the Dalian sea urchin breeding base. Ciliates were found in the body fluids of sea urchins, and 1 kg of Eucalyptus eucalyptus essential oil was mixed with 1 ton of feed, and the sea urchins were continuously fed according to normal feeding management, 3 times a day. Seven days later, the parasites of the sea urchin were checked again, and it was found that there were no ciliates in the body fluid of the sea urchin, indicating that the essential oil of Eucalyptus multiflorum had a significant deworming effect on the sea urchin.

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Abstract

The invention provides an application of Eucalyptus polybractea essential oil and its composite microbial agent in the preparation of a vermifuge for echinoderms. The Eucalyptus polybractea essentialoil composite microbial agent comprises Eucalyptus polybractea essential oil and Streptomyces griseus HL-50 with the bacterial content of 5 * 10<8> CFU / g or above, wherein the preservation number of the Streptomyces griseus HL-50 is CGMCC No.18424, and the most main effective component of the Eucalyptus polybractea essential oil is 1,8-cineole. The Eucalyptus polybractea essential oil composite microbial agent is simple to prepare and use, so effective repelling of various parasites infecting the echinoderms and bacterium inhibition can be achieved by only uniformly mixing an edible feed for the echinoderms with the composite microbial agent according to a ratio of 0.5-1.5 kg / t and continuously feeding the echinoderms with the obtained mixture for 4-7 d; and the composite microbial agent is nontoxic and harmless, is suitable for the echinoderms to use for a long term, can reduce the breeding cost, and has a wide application prospect.

Description

Technical field [0001] The invention belongs to the technical field of aquaculture, and specifically relates to the application of a multi-bud eucalyptus eucalyptus essential oil and a composite microbial agent thereof in the preparation of an insect repellent for echinoderms. Background technique [0002] Essential oils are volatile aromatic substances extracted from the flowers, leaves, stems, roots or fruits of plants through steam distillation, extrusion, cold soaking or solvent extraction. Most of them have a lighter specific gravity than water. The relative density is generally 0.8 to 1.17. Essential oils are sensitive to heat and light, so they need to be stored in dark containers and in a cool place. Because of their special aroma and solubility in organic solvents, essential oils are now mostly used in skin care products and aromatherapy, but plant essential oils function It has a variety of functions. In addition to the traditional fragrance, soothing and invigorating ...

Claims

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Application Information

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IPC IPC(8): A01N27/00A01N31/04A01N35/06A01N63/04A01N63/00C11B9/02A01P17/00
CPCA01N27/00A01N31/04A01N35/06A01N63/00C11B9/022C11B9/027Y02A50/30
Inventor 曹爱智籍立民娄倩倩
Owner SHANDONG LONGCHANG ANIMAL HEALTH PROD
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