MsrA gene and protein of white tip shark as well as method for extracting gene

An extraction method and whitetip shark technology, applied in the field of genetic engineering, can solve the problems of inability to obtain the MsrA gene sequence, protein sequence and protein expression, etc., and achieve repairing damage to methionine oxidation, high expression and purification, and improved The effect of efficiency

Active Publication Date: 2019-12-10
SHENZHEN UNIV
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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for extracting the MsrA gene, protein and gene of whitetip sharks, so as to solve the existing problem that the MsrA gene sequence, protein sequence and problems with protein expression

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  • MsrA gene and protein of white tip shark as well as method for extracting gene
  • MsrA gene and protein of white tip shark as well as method for extracting gene
  • MsrA gene and protein of white tip shark as well as method for extracting gene

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Embodiment Construction

[0023] The present invention provides a method for extracting the MsrA gene, protein and gene of the whitetip shark. In order to make the purpose, technical solution and effect of the present invention clearer and clearer, the present invention will be further described in detail below. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0024] The MsrA gene of a whitetip shark according to the present invention, wherein the nucleotide sequence of the MsrA gene is shown in SEQ ID No: 1 in the sequence listing.

[0025] In the present invention, the full length of the MsrA gene of the whitetip shark is 1207bp, wherein the 5'UTR is 20bp, the 3'UTR with the Ploy A tail is 503bp long, and the ORF is 684bp. The MsrA gene of the whitetip shark encodes 227 amino acids.

[0026] A protein expressing the MsrA gene of the whitetip shark of the present invention, wherein the amino acid...

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Abstract

The invention discloses an MsrA gene and a protein of a white tip shark as well as a method for extracting the gene. A nucleotide sequence of the MsrA gene is shown in SEQ ID No:1 of a sequence table.According to the MsrA gene and the protein of the white tip shark as well as the method for extracting the gene disclosed by the invention, under the condition of no need of full sequencing of the gene of the white tip shark, the MsrA gene sequence having pertinence performance can be conveinently provided, so that the cloning efficiency of non-mode biological genes is improved; and meanwhile, the expression level and the purification degree of MsrA protease of the white tip shark are high, and a subsequent research proves that the MsrA gene has an obvious function of recovering oxidative damage of methionine in vivo or in vitro.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a method for extracting the MsrA gene, protein and gene of whitetip sharks. Background technique [0002] The sulfur-containing amino acid methionine in protein is one of the amino acids that are easily oxidized. After methionine is oxidized, two types of methionine sulfoxide, S-type and R-type, are produced. The former can be specifically oxidized by methionine Acid sulfoxide reductase A (MsrA) reduces to methionine. MsrA is generally located in mitochondria and has become an important member of the antioxidant system of organisms because of its important role in resisting oxidative stress and protein repair. MsrA appeared 3 billion years ago and is very primitive in evolution. Sharks are primitive marine organisms that are highly conserved in evolution. Healthy sharks swim all their lives, and it is speculated that they may have a stronger antioxidant system. Co...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12N15/10C07K14/46
CPCC07K14/461C12Q1/686C12Q2521/107C12Q2537/165
Inventor 王勇程思明孙艳梅倪嘉缵
Owner SHENZHEN UNIV
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