Euphorbia lathyris callus cell suspension culture method capable of extracting and obtaining ingenol

Abstract

The invention provides an euphorbia lathyris callus cell suspension culture method capable of extracting and obtaining ingenol. The euphorbia lathyris callus cell suspension culture method capable ofextracting and obtaining the ingenol comprises the following steps: 1) inoculating a callus induction medium with roots of aseptic seedlings of euphorbia lathyris as an explant, and carrying out culturing so as to obtain callus; 2) cutting the callus into small pieces, inoculating a subculture medium with the cut callus, and carrying out subculture; 3) shredding the callus after the subculture, inoculating a liquid medium used for carrying out suspension culture with the shredded callus, and carrying out oscillation culture; 4) renewing culture solution used for carrying out the suspension culture, and carrying out subculture; and 5) collecting suspension cells as a raw material, and carrying out separation and preparation so as to obtain the ingenol and lathyrol. The euphorbia lathyris suspension cells obtained by the euphorbia lathyris callus cell suspension culture method capable of extracting and obtaining ingenol are fast in proliferation rate; and moreover, the ingenol and the lathyrol can be stably extracted from the euphorbia lathyris suspension cells, thereby providing technical support for large-scale euphorbia athyris cell culture and industrial production of ingenol biosynthesis. Thus, the euphorbia lathyris callus cell suspension culture method capable of extracting and obtaining ingenol has important application values.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for suspension culture of Caper caper callus cells capable of extracting ingenol. Background technique [0002] Euphorbia lathryis L. is a biennial herbaceous plant of Euphorbiaceae (Euphorbiaceae). It is native to Europe and widely distributed in my country. The oil content of its seeds is as high as 45%. It has the potential to replace petroleum as a new energy source. An ideal energy plant. Simultaneously, the dried seeds of Scaper persicae (Semen chinensis) are traditional Chinese medicines in my country, which have the effects of breaking blood and dispelling stagnation, and detumescence by removing water. Modern medical research has found that Caper caper extract also has anti-tumor, anti-cancer and whitening effects. In recent years, researchers at home and abroad have discovered pharmacologically active ingredients such as anti-cancer and anti-AIDS ...

AI Technical Summary

Problems solved by technology

However, ingenol is only distributed in a small amount in a few plants of the genus Euphorbia, such as Capsophila, and the yield of extracting and isolating from Capers is only 0.03%, which is nearly 17% lower than the yield of artemisinin directly isolated from plants. times, so the cost of semi-synthesis to finally obtain ingenol methacrylate is also high

In 2013, LEO Pharmaceuticals and the Scripps Research Institute of the United States studied a 14-step total synthesis method for ingenol, but it needs to rely on chiral catalysts, and the yield is only about 1%, so the total synthesis process is difficult and costly. Difficult to industrialize production

At present, the suspension culture technology of caperia callus cells is not perfect enough, and the effect of regulating the synthesis of its secondary metabolites is not satisfactory. Optimizing the ratio of each component in the medium and other ways to find out a cell suspension culture method that can stably extract and obtain the target product