Method for preparing functional grease by ester exchange between frozen and separated heavy-phase rice bran oil and conjugated linoleic acid

Abstract

The invention provides a method for preparing functional grease by ester exchange between frozen and separated heavy-phase rice bran oil and conjugated linoleic acid. The method comprises the following steps of taking white clay as a crystallizing agent; carrying out gradient freezing centrifugation to enable saturated fatty acid of the rice bran oil to be enriched; and carrying out ester exchangeon the conjugated linoleic acid under the catalysis of Novozym 435 lipase to prepare the functional grease. The recycling problem of an organic solvent is solved, and the high value of rice bran is improved. With the improvement of the unsaturation degree, the thermal stability of an unsaturated fatty acid complex is gradually reduced, and due to the influence of factors such as crystallization temperature and conditions, different structural components in mixed fatty acid can be separated and enriched. The technological conditions of gradient freezing centrifugation separation of the rice bran oil are selected; and a result shows that the optimal conditions for preparing the functional grease are that the adding amount of the lipase is 9%, the reaction temperature is 60 DEG C, the reaction time is 24 hours, the obtained grease is rich in phytosterol, oryzanol and tocopherol, and the CLA esterification rate is 38%.

Description

technical field [0001] The invention relates to a method for preparing functional oil by transesterifying heavy phase rice bran oil after freezing and separation with conjugated linoleic acid, in particular to a gradient frozen centrifugal rice bran oil, wherein the separated heavy phase rice bran oil is transesterified with conjugated linoleic acid In the method for preparing functional oil, the heavy phase rice bran oil contains tocopherol and phytosterol, which improves the high-value quality of the rice bran oil, inhibits the harm of solvent fractionation to the product, and reduces the production cost. Background technique [0002] Rice bran oil is the main by-product of rice processing. Rice bran oil prepared by aqueous enzymatic method is rich in nutrients, including tocopherols, phytosterols and oryzanol. It has very important nutritional development value and has a broad market prospect. With the development of the domestic economy and the improvement of people's li...

AI Technical Summary

Problems solved by technology

[0007] The principle is that the physical properties of the components in the mixed fatty acids are similar. In this case, it is difficult to achieve true separation by traditional separation methods.