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Positive plasmid molecule pYCID-1905 for identifying transgenic rape and product transformants thereof, and application

A technology of pycid-1905 and positive plasmid, which is applied in the direction of recombinant DNA technology, microbial measurement/testing, biochemical equipment and methods, etc., and can solve the problems of undiscovered positive plasmid molecules and applications

Pending Publication Date: 2019-12-03
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] After searching existing patents and other documents, no report has been found on the use of the design of the present invention or similar designs to construct transgenic rapeseed transformants to identify universal positive plasmid molecules and applications

Method used

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  • Positive plasmid molecule pYCID-1905 for identifying transgenic rape and product transformants thereof, and application
  • Positive plasmid molecule pYCID-1905 for identifying transgenic rape and product transformants thereof, and application
  • Positive plasmid molecule pYCID-1905 for identifying transgenic rape and product transformants thereof, and application

Examples

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Embodiment Construction

[0200] Below in conjunction with accompanying drawing and embodiment the present invention is described in detail:

[0201] 1. Construction of the positive plasmid molecule pYCID-1905

[0202] (1) Determination of detection targets for identification of transgenic rapeseed transformants

[0203] According to relevant information on the industrialization of transgenic rapeseed, there are 9 rapeseed transformants approved for import in my country, namely GT73, MS1, OXY235, MS8, RF3, T45, RF1, Topas 19 / 2, RF2; There are 2 bodies, namely 73496 and MON88302. The 11 transformant targets of rapeseed were determined to be used for identification and detection of transgenic rapeseed transformants. Through the results of identification and detection, it was possible to determine whether the transgenic components in the sample were from authorized transgenic rapeseed or non-authorized transgenic rapeseed.

[0204] (2) Determine the target sequence of 11 transformants

[0205] The detec...

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Abstract

The invention discloses design and application of a positive plasmid molecule pYCID-1905 for identifying transgenic rape and product transformants thereof, and relates to security supervision of the transgenic rape and a transformant identification technique in the field of transgenic security. The base sequence of the pYCID-1905 is as shown in SEQID NO.1. The pYCID-1905 is used as a positive control and quality control sample, ordinary PCR and real-time fluorescence PCR identification detection of transformants can be performed on rape and products thereof, and the coverage rate of approved transgenic rape varieties and transgenic rape varieties to be approved in China is 100%. According to the design and the application disclosed by the invention, the technical difficult problem that positive control or standard products lack in transgenic rape transformant identification and detection is solved, the problem of positive control set for each detection target during variety identification and detection is solved, and human resource cost and economic cost for preparing each rape transformant positive control are reduced.

Description

technical field [0001] The invention relates to safety supervision and transformant identification technology of transgenic rapeseed in the field of transgenic safety, in particular to the design and application of a positive plasmid molecule pYCID-1905. Background technique [0002] Over the past 20 years, the global R&D and commercialization of GMOs have developed rapidly. Since 1992, 4,133 GMO products have been approved for regulatory approval worldwide, involving 476 transformants of 26 GMO crops, with a cumulative planting area of ​​more than 2.3 billion hectares. huge economic benefits. At the same time, the safety of genetically modified organisms has always attracted the attention of all walks of life. For this reason, more than 50 countries and regions, including China, the European Union, the United States, Japan and South Korea, have promulgated and implemented laws and regulations on the safety management of genetically modified organisms. Regulate the R&D and ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/686C12N15/11
CPCC12Q1/6895C12Q1/686C12Q2600/166C12Q2565/125C12Q2545/113C12Q2561/113C12Q2563/107
Inventor 武玉花张秀杰李俊李夏莹王灏潜翟杉杉陈子言张洪文高鸿飞李允静吴刚
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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