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MUC16-targeting single-strand antibody, and MUC16-targeting chimeric antigen receptor T cell and preparation method and application thereof

A chimeric antigen receptor, CAR-MUC16 technology, applied in the field of medical biology, can solve the problem of low cure rate of ovarian cancer and achieve the effects of efficient and specific killing, promotion of amplification, and strong affinity activity

Inactive Publication Date: 2019-12-03
SHENZHEN BINDEBIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the cure rate of ovarian cancer is generally low

Method used

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  • MUC16-targeting single-strand antibody, and MUC16-targeting chimeric antigen receptor T cell and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] A method for preparing a chimeric antigen receptor T cell targeting MUC16, comprising the following steps:

[0077] (1) Prepare the gene sequence of the chimeric antigen receptor CAR-MUC16 targeting MUC16

[0078]The coding genes of the signal peptide, the single-chain antibody targeting MUC16, the CD8α hinge region, the CD8 transmembrane region, the 4-1BB signal region and the CD3ζ signal region are respectively prepared, and the coding genes of the signal peptide are shown in SEQ ID NO: 14 , the coding gene of the single-chain antibody targeting MUC16 is shown in SEQ ID NO: 2, the coding gene of the CD8α hinge region is shown in SEQ ID NO: 6, and the coding gene of the CD8 transmembrane region is shown in SEQ ID NO: As shown in ID NO: 8, the coding gene of the 4-1BB signal region is shown in SEQ ID NO: 10, and the coding gene of the CD3ζ signal region is shown in SEQ ID NO: 12.

[0079] The genes encoding the above-mentioned signal peptide, single-chain antibody targ...

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PUM

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Abstract

The invention provides an MUC16-targeting single-strand antibody. The amino acid sequence of the MUC16-targeting single-strand antibody comprises an amino acid sequence as shown in SEQ ID NO:1. The invention further provides a chimeric antigen receptor T cell including the MUC16-targeting single-strand antibody. The MUC16-targeting chimeric antigen receptor can specifically target MUC16, can promote amplification of T cells in patient bodies, can efficiently and specifically kill tumor cells and can better maintain vitality and lethality of the cells. The invention further provides a preparation method and application of the MUC16-targeting chimeric antigen receptor T cell.

Description

technical field [0001] The invention relates to the field of medical biology, in particular to a single-chain antibody targeting MUC16, a chimeric antigen receptor T cell, and a preparation method and application thereof. Background technique [0002] Ovarian cancer is the most lethal gynecological malignancy in the world. Due to the absence of obvious symptoms and the lack of scientifically credible detection methods, most patients are diagnosed with advanced cancer. Therefore, the cure rate of ovarian cancer is generally low. MUC16 is a transmembrane protein and a member of the mucin (MUC) family. The expression of MUC16 in cervical cancer, pancreatic cancer, and ovarian cancer tissues all increased, and it is an important marker of ovarian cancer, which can be used for early diagnosis of ovarian cancer, detection of treatment effect, and evaluation of tumor recurrence. Studies have shown that MUC16 can inhibit apoptosis by interacting with tumor necrosis factor-related ...

Claims

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Application Information

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IPC IPC(8): C07K16/30C12N5/10C12N15/867A61K35/17A61P35/00
CPCA61K35/17A61P35/00C07K14/7051C07K16/3092C07K2317/622C07K2319/02C07K2319/03C07K2319/33C12N15/86C12N2510/02C12N2740/15043
Inventor 万晓春李欣刘绿艳唐超
Owner SHENZHEN BINDEBIOTECH CO LTD
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