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Preparation method of anti-tumor effective part in hibiscus tiliaceus and application thereof

An effective part, anti-tumor technology, applied in the field of medicine and biology, can solve problems such as lack, and achieve the effects of easy operation, simple preparation method and low cost

Active Publication Date: 2019-12-03
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI HAINAN BRANCH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of the extraction process of active ingredients, there is still a lack of a method that can simultaneously extract multiple active ingredients

Method used

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  • Preparation method of anti-tumor effective part in hibiscus tiliaceus and application thereof
  • Preparation method of anti-tumor effective part in hibiscus tiliaceus and application thereof
  • Preparation method of anti-tumor effective part in hibiscus tiliaceus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for preparing an anti-tumor effective part of Hibiscus chinensis, comprising the following steps:

[0034] (1) Take the trunk of the hibiscus officinalis, dry it in the shade, and remove the miscellaneous trees; cut the hibiscus officinale into small sections, and crush it into 0.5-2cm;

[0035] (2) Use an organic solvent (dichloromethane) to heat and reflux for 6 to 12 hours, and reflux and extract 3 times;

[0036] (3) Filtrate to obtain the total extract of Hibiscus japonica, concentrate under reduced pressure until the fluid paste density is 0.9g / ml, to obtain the total extract;

[0037] (4) Take the obtained total extract, use silica gel dry method to mix the sample, after the organic solvent evaporates to dryness, grind it finely, use silica gel (200-300 mesh) column chromatography, use petroleum ether as eluent, elute 3 The petroleum ether part was obtained by the column volume, and then dichloromethane-methanol (40:1) was used as the eluent to elute fo...

Embodiment 2

[0040] A method for preparing an anti-tumor effective part of Hibiscus chinensis, comprising the following steps:

[0041] (1) Take the trunk of the hibiscus officinalis, dry it in the shade, and remove the miscellaneous trees; cut the hibiscus officinale into small sections, and crush it into 0.5-2cm;

[0042] (2) Use organic solvent (acetone) to extract by cold soaking for 24 hours, and extract 3 times;

[0043] (3) Filtrate to obtain the total extract of Hibiscus japonica, concentrate under reduced pressure until the fluid paste density is 1.2g / ml, to obtain the total extract;

[0044] (4) The obtained total extract was mixed with silica gel dry method, and after the organic solvent was evaporated to dryness, it was ground, and silica gel (200-300 mesh) column chromatography was used to elute 10 samples with petroleum ether as the eluent. The petroleum ether part was obtained by the column volume, and then dichloromethane-methanol (40:1) was used as the eluent to elute for...

Embodiment 3

[0047] The main difference between embodiment 3 and embodiment 2 is:

[0048] In step (5), when performing macroporous adsorption resin elution, the chromatographic column is sequentially filled with D101, D201 and D411 type macroporous adsorption resins (mass ratio is 4:1:2).

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PUM

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Abstract

The invention relates to a preparation method of an anti-tumor effective part in hibiscus tiliaceus and an application thereof. The method comprises the steps of crushing, organic solvent extraction,concentration, silica gel column chromatography, macroporous adsorption resin elution, drying and the like. The method is simple and easy to operate, impurities in the obtained effective part are obviously reduced, and the UPLC detection result shows that the content of medicinal substances reaches 70% or above and meets the 50% content standard specified by national five types of new drugs. The pharmacodynamic research is carried out on the obtained effective part, and the result shows that the effective part has anti-proliferative activity on various tumor cells, which prompts that the effective part has a good application prospect in anti-tumor drugs or health care products.

Description

technical field [0001] The invention relates to a preparation method and application of an anti-tumor effective part of hibiscus hibiscus, belonging to the field of medical biotechnology. Background technique [0002] Hibiscus tiliaceus, belonging to the genus Hibiscus of Malvaceae, is a semi-mangrove plant with important ecological, medicinal and ornamental values, widely distributed in China, Vietnam, Cambodia, Myanmar, India, Indonesia, Malaysia, the Philippines, Laos and other tropical regions, our country is mainly produced in Hainan, Guangxi, Taiwan, Guangdong, Fujian and other provinces, and has been widely planted and cultivated. According to the "National Compilation of Chinese Herbal Medicine", Huang Hibiscus has the effects of clearing away heat and relieving cough, detoxifying and reducing swelling. It is used as medicine in coastal residential areas of Hainan for poisonous snake bites, carbuncle sores, and bronchitis. Because it can survive in the intertidal zo...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61K31/343A61P35/00
CPCA61K36/185A61K31/343A61P35/00A61K2236/35A61K2236/51
Inventor 陈德力马国需刘洋洋李光李宜航许旭东孙晓波
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI HAINAN BRANCH
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