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Method for simultaneous rapid detection of various sugars, sugar alcohols and alcohols in beer

A technology for beer and sugar alcohol, applied in the field of analytical chemistry, can solve the problems of inability to detect multiple components at the same time, unsuitable for accurate quantitative analysis, small number of components, etc., achieve good economic benefits and social application value, and reduce testing manpower and material resources. Cost, effect of improved accuracy and precision

Active Publication Date: 2019-11-29
日照海关综合技术服务中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology helps detect more efficiently and accurately different types of substances found naturally during fermentation processes like glucose syrup production. It also includes techniques for separating these compounds from other materials while maintaining their purity. Overall, this process provides valuable data sources with high efficiency and low costs compared to existing methods.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving the accuracy and reliance of identifying various chemical compounds during processing of beers due to limitations associated with existing techniques like liquor colorimetry and mass spectral analysis.

Method used

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  • Method for simultaneous rapid detection of various sugars, sugar alcohols and alcohols in beer
  • Method for simultaneous rapid detection of various sugars, sugar alcohols and alcohols in beer
  • Method for simultaneous rapid detection of various sugars, sugar alcohols and alcohols in beer

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Embodiment 1: a method for simultaneous detection of 23 kinds of sugars, sugar alcohols and alcohols in beer

[0047] 1. Instruments and Reagents

[0048] Ion chromatograph (Thermo Scientific ICS5000+), equipped with electrochemical detector and automatic sample injector (AS-SP); Milli-Q ultrapure water instrument (Millipore, USA); intelligent ultrasonic cleaner (Shanghai Zhixin Instrument Co., Ltd. DL-360E); electronic balance (Mettler ML802 / 02); experimental water: ultrapure water (resistivity 18.2MΩ.cm); sugar, sugar alcohol, alcohol standard or standard reagent (purity>98% ); sodium hydroxide solution (50% w / w, Fisher Chemical); hydrochloric acid (excellent grade); IC-RP10 column (Agela Technologies); IC-H10 column (Agela Technologies).

[0049] 2. Ion Chromatography Conditions

[0050] Chromatographic separation column: Dionex CarboPac TM MA1 (4×250 mm), guard column: Dionex CarboPac TM MA1 (4×50 mm); integrated pulse amperometric detection; working electrode: ...

Embodiment 2

[0058] Example 2: Influence of flow rate on detection

[0059] According to the conditions of the experimental analysis in Example 1, under the condition that other conditions remain unchanged, the flow rate of the eluent is successively tested to be 0.30 mL / min, 0.40 mL / min, 0.45 mL / min, and 0.50 mL / min. The component separation of the standard solution, the ion chromatograms of the mixed standard solution at different flow rates are as follows: figure 2 Shown; The relationship between the eluent flow rate and the system pressure is shown in Table 2.

[0060] Table 2 Relationship between eluent flow rate and system pressure

[0061]

[0062] Will figure 2 Combined with Table 2, it can be concluded that the greater the flow rate of the eluent, the greater the system pressure, and the faster the peak time of the components, the flow rate increased from 0.30 mL / min to 0.50 mL / min, and the total peak time of the 23 components From 90 minutes to 62 minutes gradually, while...

Embodiment 3

[0063] Example 3: Influence of multi-stage gradient elution conditions on detection

[0064] According to the conditions of the experimental analysis in Example 1, the separation of the mixed standard solution components was tested successively under the conditions of the initial eluent concentrations of 450, 470, 480, 490, and 500 mmol / L under the condition of other conditions unchanged. , the component separation of the mixed standard solution is as follows image 3 shown.

[0065] From image 3 It can be seen from the figure that when the initial eluting concentration is 450 mmol / L, the separation degree of components 7 and 8, components 19 and 20 is not high, and components 10 and 11 are almost overlapped; when the initial eluting concentration is 470 mmol / L , the resolution of components 10 and 11 was not high; when the initial eluting concentrations were 490 and 500 mmol / L, the resolution of components 14 and 15 decreased significantly, while the separation of componen...

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Abstract

The invention discloses a method for simultaneous rapid detection of various sugars, sugar alcohols and alcohols in beer, belonging to the technical field of analytical chemistry. In the method provided by the invention, an integrated pulsed amperometric-ion chromatography is used, by optimization of a pretreatment detection method, experimental factors influencing separation of constituents are researched, and a method for simultaneously detecting 23 types of sugars, sugar alcohols and alcohols (2, 3-butanediol, ethyl alcohol, methanol, glycerin, erythritol, xylitol, fucose, arabitol, sorbitol, trehalose, ribovitol, mannitol, 2-DNA-D-glucose, mannose, melibiose, glucose, maltitol, fructose, lactose, ribose, sucrose, raffinose and maltitol ) in the beer is built one time. Thus, a technicalproblem of accurate analysis on multiple constituents including the sugars, the sugar alcohols and the alcohols in the beer is solved, the method provided by the invention is rapid, accurate and sensitive, realizes simultaneous analysis of multiple types and multiple constituents, and makes an important contribution to detection of beer constituents.

Description

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Claims

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Application Information

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