Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Sh2b adapter protein 3 for the prediction of bone marrow response and immune response

A technology of SH2B3, immune response, applied in the determination/inspection of microorganisms, biochemical equipment and methods, complex mathematical operations, etc., can solve problems such as unsolved problems, and achieve the effect of reducing costs and adverse drug reactions

Pending Publication Date: 2019-11-26
UNIVERSITY OF ROSTOCK
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Thus, the question of the mechanism of cardiac regeneration and the role of bone marrow stem cells in regulating angiogenesis remains unresolved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sh2b adapter protein 3 for the prediction of bone marrow response and immune response
  • Sh2b adapter protein 3 for the prediction of bone marrow response and immune response
  • Sh2b adapter protein 3 for the prediction of bone marrow response and immune response

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0115] CD133 in myocardium + Purified autologous bone marrow stem cell (BMSC) transplantation has been investigated as an adjunct strategy to coronary artery bypass grafting (CABG) revascularization in acute myocardial ST-segment elevation infarction (STEMI) and sequentially in coronary 3-vessel disease Restoration of left ventricular cardiac function after deterioration of left ventricular ejection fraction (LVEF) following acute PCI and secondary CABG revascularization. Previous safety and efficacy (Phase I, IIa, IIb) trials have demonstrated enhancement of left ventricular ejection fraction (LVEF) and adjuvant CD133 + Clinical safety of BMSC therapy on coronary revascularization. Design a randomized double-blind placebo-controlled clinical trial to evaluate clinical safety, efficacy and biomarkers for interventional CD133 + Identification of CD133 in BMSC transplantation + Cardiac repair mechanisms associated with bone marrow stem cells.

[0116] Conduct a randomized do...

example 2

[0126] Measurement of SH2B3 gene expression was performed by whole blood sampling followed by RT-PCR.

[0127] Table 1: Measurement of SH2B3 gene expression by RT-PCR

[0128]

example 3

[0130]Native samples of peripheral blood (EDTA blood) were used for quantitative real-time-PCR using LightCycler 480II (Roche Deutschland Holding GmbH).

[0131] RNA was isolated from 1 ml whole blood aliquots (stored at -80°C) using the GeneJET Stabilized and Fresh Whole Blood RNA Kit (Thermo Fisher Scientific). Reverse transcription was performed using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). Using the StepOnePlusRT PCR system (Applied Biosystems TM ) for RT-PCR. Using cDNA (30ng per reaction), Universal PCR Master Mix (Thermo Fisher Scientific) and SH2B3 GeneExpression Assay (Hs01081959_g1, Thermo Fisher Scientific). Three replicates of the process were performed. To calculate the relative expression ratio of SH2B3, the ΔΔCT method was employed.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to SH2B adapter protein 3 (SH2B3) for use as a diagnostic marker. Further, the present invention relates to a method for the determination of SH2B3 gene expression.

Description

technical field [0001] The present invention relates to a SH2B adapter protein 3 (SH2B3) useful as a diagnostic marker. Furthermore, the present invention relates to a method for determining the expression of the SH2B3 gene. Background technique [0002] SH2B3, also known as lymphocyte adapter protein (LNK), is a protein encoded by the SH2B3 gene on chromosome 12 in humans. It is ubiquitously expressed in many tissues and cell types ("BioGPS your GenePortal System". biogps.org. Retrieved 2016-10-11). SH2B3 acts as a regulator in signaling pathways related to hematopoiesis, inflammation and cell migration (Devallière J, Charreau B (November 2011). Biochemical Pharmacology.82(10):1391–402.doi:10.1016 / j.bcp.2011.06 .023.PMID21723852). Thus, it is involved in blood disorders, autoimmune diseases and vascular diseases (Auburger G, Gispert S, Lahut S, Omür O, Damrath E, Heck M, N (June 2014). Wourld Journal of Diabetes. 5(3):316–27. doi:10.4239 / wjd.v5.i3.316.PMC 4058736.PMID ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6813C12Q1/6883
CPCC12Q1/6813C12Q1/6883C12Q2600/112C12Q2600/118C12Q2600/158C12Q2600/178C12Q1/6851C12Q1/686G06F17/18
Inventor G·施泰因霍夫
Owner UNIVERSITY OF ROSTOCK
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com