A kind of floating feed for snakehead larvae and preparation method thereof
A feed and seed technology, which is applied in the field of floating feed and its preparation for juvenile snakehead fish, can solve problems such as differences in the effect of surfactants, achieve the effects of less nutritional antagonistic factors, promote uniform dispersion, and improve the survival rate of snakehead seedlings
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Embodiment 1
[0048] The preparation of embodiment 1 spraying agent
[0049] Mix 85% fish oil, 3% lipopeptide biosurfactant, 2% vitamin E and the rest of distilled water; stir for 5-15 minutes to obtain the spraying agent.
[0050] Wherein, the lipopeptide biosurfactant is obtained by fermenting and purifying the modified Bacillus thuringiensis, and its chemical structural formula is:
[0051]
[0052] Among them, Phe, Pro, Thr, Ala and Tyr represent phenylalanine, proline, threonine, alanine and tyrosine, respectively.
[0053] Wherein, the transformation method of Bacillus thuringiensis is as follows: by ultraviolet mutagenesis, plasma mutagenesis, ethyl methanesulfonate (EMS) mutagenesis and compound mutagenesis methods thereof, Bacillus thuringiensis (Bacillus thuringiensis) is carried out mutagenesis, Taking the yield of lipopeptide surfactant as the screening index, the mutant strain with high yield was screened. The yield of lipopeptide surfactants of Bacillus thuringiensis (0.3...
Embodiment 2
[0060] The preparation of embodiment 2 spraying agent
[0061] Mix 87% fish oil, 2.5% lipopeptide biosurfactant, 1.6% vitamin E and the rest of distilled water; stir for 5-15 minutes to obtain the spraying agent.
Embodiment 3
[0062] The preparation of embodiment 3 spraying agent
[0063] Mix 89% fish oil, 1.5% lipopeptide biosurfactant, 0.8% vitamin E and the rest of distilled water; stir for 5-15 minutes to obtain the spraying agent.
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