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Application of heparin sodium in preparation of diluent for goat sperm cryopreservation and preparation method

A technology for cryopreservation and dilution, which can be used in applications, preservation of human or animal bodies, animal husbandry, etc., and can solve problems such as low sperm motility

Active Publication Date: 2019-11-22
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the cryopreservation technology of goat semen has been greatly developed, the problem of low sperm motility after freezing-thawing treatment still exists

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] This embodiment provides a diluent for goat sperm cryopreservation, including sodium heparin, fructose, trisodium citrate, citric acid, sodium bicarbonate, lecithin, dimethyl sulfoxide, ethylene glycol, penicillin and streptomycin .

[0029] The preparation of the diluent for cryopreservation of the above-mentioned goat sperm comprises the following steps:

[0030] (1) Preparation of liquid A: Accurately weigh 0.25 g of trisodium citrate, 0.75 g of citric acid, and 0.062 g of sodium bicarbonate with an analytical balance, add 50 mL of sterile ultrapure water to dissolve, and use 1 mol / L sodium hydroxide or 1 mol / L Adjust the pH to 7 with L hydrochloric acid, and adjust the volume to 100 mL to obtain the so-called A liquid.

[0031] (2) Preparation of liquid B: accurately weigh 0.50 g of fructose and 0.125 g of lecithin with an analytical balance, accurately measure 6 mL of dimethyl sulfoxide and 6 mL of ethylene glycol, add 50 mL of liquid A to dissolve, and use 1mol / L...

Embodiment 2

[0047] This embodiment provides a diluent for goat sperm cryopreservation, including sodium heparin, fructose, trisodium citrate, citric acid, sodium bicarbonate, lecithin, dimethyl sulfoxide, ethylene glycol, penicillin and streptomycin .

[0048] The preparation of the diluent for cryopreservation of the above-mentioned goat sperm comprises the following steps:

[0049] (1) Preparation of liquid A: Accurately weigh 0.25 g of trisodium citrate, 0.75 g of citric acid, and 0.062 g of sodium bicarbonate with an analytical balance, add 50 mL of sterile ultrapure water to dissolve, and use 1 mol / L sodium hydroxide or 1 mol / L Adjust the pH to 7 with L hydrochloric acid, and adjust the volume to 100 mL to obtain the so-called A liquid.

[0050] (2) Preparation of liquid B: accurately weigh 4g of fructose and 1g of lecithin with an analytical balance, accurately measure 30mL of dimethyl sulfoxide and 30mL of ethylene glycol, add 50mL of liquid A to dissolve, and use 1mol / L sodium hy...

Embodiment 3

[0064] This embodiment provides a diluent for goat sperm cryopreservation, including sodium heparin, fructose, trisodium citrate, citric acid, sodium bicarbonate, lecithin, dimethyl sulfoxide, ethylene glycol, penicillin and streptomycin .

[0065] The preparation of the diluent for cryopreservation of the above-mentioned goat sperm comprises the following steps:

[0066] (1) Preparation of liquid A: Accurately weigh 2 g of trisodium citrate, 6 g of citric acid, and 0.5 g of sodium bicarbonate with an analytical balance, add 50 mL of sterile ultrapure water to dissolve, and use 1 mol / L sodium hydroxide or 1 mol / L Adjust the pH to 7 with hydrochloric acid, and adjust the volume to 100mL to obtain the so-called A solution.

[0067] (2) Preparation of liquid B: accurately weigh 4 g of fructose and 1 g of lecithin with an analytical balance, accurately measure 6 mL of dimethyl sulfoxide and 6 mL of ethylene glycol, add 50 mL of liquid A to dissolve, and use 1 mol / L sodium hydroxi...

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PUM

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Abstract

The invention discloses an application of heparin sodium in preparation of a diluent for goat sperm cryopreservation and a preparation method, and also provides a diluent for goat sperm cryopreservation, which comprises heparin sodium, nutrient substances, buffer substances, a cryoprotectant and antibacterial substances. The diluent for goat sperm cryopreservation plays a role in protecting spermsin the processes of sperm cooling, cryopreservation and unfreezing, damage is reduced, and the sperm motility and acrosome integrity rate of goat frozen sperms are obviously improved.

Description

technical field [0001] The invention belongs to the field of animal biotechnology and relates to animal reproduction technology, in particular to the application and preparation method of heparin sodium for preparing diluent for goat semen preservation. Background technique [0002] Semen cryopreservation is one of the key links in the animal biotechnology system, which mainly solves the problem of long-term stable storage of semen. Through the artificial collection and long-term storage of semen, the semen produced by animals can be used across time, across regions, and in various ways, thus greatly improving the use value of male animals in the field of actual production and scientific research. For example, in the field of animal breeding, it is of great significance for estimation of breeding value, protection of germplasm resources, variety improvement, breeding of new varieties, realization of factory-scale farming, etc., as well as reducing production costs and improv...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0215
Inventor 李广王建刚安小鹏张磊张效川郭丹补琪琪杨少华
Owner NORTHWEST A & F UNIV
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