Preparation method of silicosis mouse models

Abstract

The invention relates to a preparation method of silicosis mouse models. The preparation method of the silicosis mouse models is characterized in that after experiment preparation, isoflurane in an anesthesia machine is adopted to anesthetize mice in a model group, 3 seconds after rapid-shallow breaths of the mice become deep and slow, each mouse is taken out and placed at the center of a palm, the tip of a thumb presses against the underjaw of each mouse, a micropipette is adopted to instill a SiO2 micro-sized particle suspension through the nose of each mouse, the SiO2 micro-sized particle suspension is instilled into the nose wings to be absorbed, closed-chest massage is conducted for 2-3 minutes, then the mice are placed into cages, and after an experiment is finished, the mice are sent back to a normal-temperature animal feed room; only normal saline of the same volume is used for conducting nasal inhalation on mice in a contrast group; weight indexes of the mice are obtained before and after the experiment, the mice are killed at the first week, the second week, the fourth week, the eighth week and the sixteenth week respectively, lung tissue is taken to be pathologically andbiochemically compared and identified, and a model preparation effect is evaluated. According to the preparation method of the silicosis mouse models, a model production technology is stable and efficient; the method can be repeated many times and is noninvasive, environmentally friendly and humanistic, and labor and time are saved.

Description

technical field [0001] The invention relates to the field of experiment technology, in particular to a method for preparing a mouse model of silicosis. Background technique [0002] Silicosis is an irreversible idiopathic pulmonary fibrosis occupational disease caused by long-term exposure to dust environment. In recent years, ceramics, building materials, mining and other industries have adopted various measures to reduce dust and preventive measures, but silicosis still occurs among occupational groups. In the field of modern energy and safety, the prevention and clinical treatment of silica dust occupational diseases, there are still many major clinical difficult problems that need to be explored and solved urgently. It is urgent to strengthen relevant basic research, such as: the pathological change mechanism of silicosis, the treatment of silicosis and the prevention of silicosis Wait. [0003] In the process of clinical and basic research, issues such as ethics and r...

AI Technical Summary

Problems solved by technology

[0004] In the past, the preparation of mouse silicosis models was mainly through surgical invasive methods, the risk of infection was difficult to control, and deep injection anesthesia was required for mice, the mortality rate of mice was high, and the repeatability of experiments was poor

In recent years, some researchers have prepared by intratracheal instillation, but the silicosis model produced by this method has the following disadvantages: ① Airway invasion will cause damage to the airway of mice, increasing the external factors that induce silicosis; ②The instillation in the airway does not conform to the breathing rhythm of the mice, and the mouse’s respiratory compliance will be poor during the operation, which increases the risk of death; ③The airway instillation has the possibility of entering the esophagus by mistake, which increases the failure rate of the experiment

So far, there is no mouse model of silicosis with safe operation, good reproducibility, and precise control of injury and fibrosis outcome

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