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Reagent and kit for rapid detection of human helicobacter pylori

A technology for detection reagents and kits, which is applied to measurement devices, material analysis, instruments and other directions by observing the influence on chemical indicators, can solve the problems of long detection time, low accuracy, and large pH measurement error, etc. Achieve short detection time, rapid detection, high accuracy and specificity

Active Publication Date: 2019-11-19
山东宝灵生物医药科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the pH measurement error of this detection method is large, while the activity is low and the detection time is long
[0007] In short, the above-mentioned rapid urease test methods all have technical defects such as low accuracy, long detection time and low specificity to varying degrees.

Method used

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  • Reagent and kit for rapid detection of human helicobacter pylori

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1 prepares detection reagent

[0052] First prepare monodisperse high stability and highly active metal nickel nanoparticles, the average particle diameter D50 is 28nm; then get 0.05g to prepare the metal nickel ethanol solution / dispersion of 0.1mg / mL; add urea subsequently to obtain a concentration of A mixed solution of 0.2g / mL urea and 0.1mg / mL nano-nickel.

Embodiment 2

[0053] Embodiment 2 prepares kit

[0054] Such as figure 1 As shown, the main structure of the kit in Example 2 includes: 1-result observation area; 2-test paper; 3-sampler; 4-detection reagent; 5-reactor; 6-sampling scale line; 7-top cover switch; 8- Top cover.

[0055] Wherein, the detection reagent is from the detection reagent of Example 1.

[0056] Use a sampler to add about a certain volume of diluted saliva sample to the scale in the sample pool, close the lid tightly, after a certain period of reaction, turn it over, and observe the color change of the test paper through the observation area. If there is a color change (turning into light red or purple red) ), it is a positive reaction.

[0057] Effect test

[0058] Different samples were used for detection, and the detection results are shown in Table 1 below.

[0059] Table 1

[0060] sample Saliva volume (ml) Response time (min) accuracy 1 0.1ml 0.5 + 2 0.1ml 1 ++ 3 0.1ml 2 ...

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Abstract

A detection reagent is provided and includes urea, metallic nickel, and a necessary solvent. In addition, a kit for detecting human helicobacter pylori is provided, and includes a sampler, a reactor,and test paper; and the reactor accommodates the detection reagent. The reagent and the kit can achieve rapid detection of the human helicobacter pylori, are high in accuracy and specificity, and areshort in detection time.

Description

technical field [0001] The invention belongs to the technical field of biological detection and relates to a reagent and kit for rapid detection of human gastric Helicobacter pylori. Background technique [0002] Gastric Helicobacter pylori (abbreviated as HP) is a spiral Gram-negative bacterium in the human gastric mucosa, which is the main pathogenic factor leading to chronic gastritis, gastric ulcer, and even gastric cancer. Epidemiological studies have shown that the positive rate of Helicobacter pylori in my country and most developing countries increases with age. In my country, the infection rate of 20-40 years old is 45.4%-63.6%, and the infection rate of people over 70 years old is as high as 78.9%. About 10% of children are positive after about 10 years of age ～ 20%, so the development of effective and rapid HP detection methods has very important and realistic clinical significance. [0003] The clinical detection methods of HP infection are mainly divided into t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/775
Inventor 王冠男
Owner 山东宝灵生物医药科技有限公司
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