Optical fiber birefringence effect-based time-resolved CARS microscopic imaging device and method

Abstract

The invention discloses an optical fiber birefringence effect-based time-resolved CARS microscopic imaging device and method. The device at least comprises a femtosecond laser, a power adjusting part,a stokes light path part, a pump light pulse and detection light pulse light path part, a short-pass dichroscope and a CARS microscopic imaging system; a part of ultrashort pulses enter a polarization maintaining optical fiber along the angular bisector of the fast axis and slow axis of the polarization maintaining optical fiber; since the refractive indexes of the fast axis and slow axis of thepolarization maintaining optical fiber are different, the fast-axis optical pulses and slow-axis optical pulses of the output end of the polarization-maintaining optical fiber under relative time delay and are respectively used as pump light pulses and detection light pulses; the other part of the ultrashort pulses are inputted into a high-nonlinearity polarization-maintaining photonic crystal fiber; wavelength red-shift optical soliton pulses are generated through a soliton self-frequency shift effect; and the wavelength red-shift optical soliton pulses are used as stokes light pulses. With the optical fiber birefringence effect-based time-resolved CARS microscopic imaging device and method of the invention adopted, the measurement of time-resolved CARS signals is realized, and at the same time, the complexity of a traditional time-resolved CARS microscopic imaging system is reduced.

Description

technical field [0001] The invention belongs to the field of nonlinear optical fiber and spectral resonance microscopic imaging, in particular to a time-resolved CARS microscopic imaging device and method. Background technique [0002] CARS microscopic imaging technology is a label-free, non-contact and chemically selective microscopic imaging technology that uses the resonance energy level information of molecules in substances to perform resonance spectrum imaging. Its essence is pump light, probe light, and the resonance energy level of the sample to be measured The four-wave mixing effect, when the frequency difference between the pump light and the Stokes light is equal to the resonance energy level of the target chemical bond in the sample to be tested, and the three meet the phase matching conditions, the anti-Stokes of the sample to be tested will be excited X Raman scattering signal, that is, CARS signal. However, in the traditional CARS microscopic imaging system,...

AI Technical Summary

Problems solved by technology

In the commonly used time-resolved CARS microscopic imaging system, in order to generate the time delay between the pump light pulse and the probe light pulse, the method of adding a pump laser or adding a spatial optical delay line is usually used, which undoubtedly increases the space optical path of the system. The complexity and the cost of operation and maintenance are not conducive to the use and development of time-resolved CARS microscopy imaging system

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