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A kind of establishment method of golden cherry root fingerprint and quality detection method of golden cherry root

A quality detection method and fingerprint technology, which are applied in the field of analysis of Chinese medicinal materials, can solve the problems of large differences in medicinal effects of golden cherry root medicinal materials, weak quality control, uneven quality of golden cherry root, etc., and achieve accurate and reliable detection, short preparation time, and characteristics. The effect of peaks

Active Publication Date: 2022-07-19
ZHUZHOU QIANJIN PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for establishing the fingerprint of rosa chinensis in view of the problem that the quality of the existing rosa chinensis is uneven and the quality control is relatively weak, resulting in large differences in the medicinal effects of roe chinensis

Method used

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  • A kind of establishment method of golden cherry root fingerprint and quality detection method of golden cherry root
  • A kind of establishment method of golden cherry root fingerprint and quality detection method of golden cherry root
  • A kind of establishment method of golden cherry root fingerprint and quality detection method of golden cherry root

Examples

Experimental program
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Effect test

Embodiment 1

[0059] Embodiment 1 The establishment method of golden cherry root fingerprint

[0060] A kind of establishment method of golden cherry root fingerprint, specifically comprises the following steps:

[0061] (1) Preparation of the test solution: add methanol at a mass-to-volume ratio of 1:50, extract by heating under reflux, make up for weight loss with methanol after cooling, shake well, filter through a 0.22 μm microporous membrane, and take the filtrate to obtain the test product solution;

[0062] (2) Configuration of reference substance

[0063] The reference substances are: pearside, rosin, salicylic acid, rosanic acid, virginic acid, ileosin B, 24-deoxy-sericoside, 2-oxo-pomeric acid, pomelic acid, ursin Fruit acid.

[0064] Preparation process: respectively take appropriate amounts of the reference substances of prickly glucoside, rosin, salicylic acid, rhodic acid, and valeric acid, accurately weigh them, and add methanol to prepare 1 mL containing 0.094 mg of prick...

Embodiment 2

[0099] The determination of embodiment 2 golden cherry root composition and content

[0100] There are many kinds of triterpenoids contained in the root of golden cherry, and through the test of Example 1, it can be known that the important triterpenoid active ingredients are prickly glucoside, rosin, salicylic acid, rhodic acid and virginic acid, therefore, According to the detection conditions of HPLC determined in Example 1, the contents of erythroside, rosin, salicylic acid, rosacidic acid, and valeric acid in 20 batches of the medicinal materials of golden cherry root were determined to evaluate the quality of golden cherry root.

[0101] 1. Determination of linear relationship

[0102] 1) Preparation of reference solution

[0103] The reference substances are: prickly pear glycosides, rosaside, salicylic acid, rosin acid, and verminic acid.

[0104] Preparation process: respectively take appropriate amounts of the reference substances of prickly glucoside, rosin, salic...

Embodiment 3

[0128] The determination of embodiment 3 golden cherry root moisture, ash and extract

[0129] In this example, the method specified in the Pharmacopoeia was used to detect the moisture, ash and leachate of 20 batches of Radix chinensis root samples. The specific detection process is as follows.

[0130] 1. Moisture determination

[0131] With reference to the moisture determination method (the second method of four general rules of the Chinese Pharmacopoeia in 2015), the specific process is:

[0132] Take 2g of golden cherry root medicinal powder, spread it in a flat weighing bottle dried to constant weight, with a thickness of no more than 5mm, accurately weigh it, open the bottle cap and dry it at 105 ° C for 5 hours, close the bottle cap, and place it in a desiccator. Let it cool for 30 minutes, accurately weigh it, then dry it at the above temperature for 1 hour, let it cool, and weigh until the difference between two consecutive weighings does not exceed 5 mg.

[0133]...

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Abstract

The invention discloses a method for establishing a fingerprint of the golden cherry root and a method for detecting the quality of the golden cherry root. The construction method includes the preparation of the test solution and the process of HPLC analysis and detection, and the fingerprint of the golden cherry root can be obtained. The construction method obtained by the invention through a large number of experiments has the advantages of simple operation, short time-consuming for the preparation of the test solution, good precision, good repeatability, good stability, many characteristic peaks, accurate and reliable detection, and can be used for the quality detection of golden cherry root medicinal materials , which provides high quality control standards for drug production and testing to ensure the stability of product quality and the safety and effectiveness of drug use. At the same time, piroside, rosin, salicylic acid, rosanic acid, and virginic acid are all important triterpenoid active components in golden cherry root, which have a great influence on the efficacy of golden cherry root. Control the content of important triterpenoid active ingredients, so as to control the quality and efficacy of golden cherry root medicinal materials.

Description

technical field [0001] The invention relates to the field of analysis of Chinese medicinal materials, and more particularly, to a method for establishing a fingerprint of Radix japonica and a method for detecting the quality of Radix Radix. Background technique [0002] Golden cherry root, also known as Golden Cherry Blossom, Tuogu Dan, etc., is the root of the dicotyledonous Rosaceae plant Golden Cherry Blossom Rosaevigata Michx. , stop diarrhea, blood and vaginal discharge." Its taste is bitter, sour, astringent, and flat. Returns to the spleen, liver and kidney meridians, and has the functions of clearing away heat and removing dampness, detoxifying and reducing swelling, promoting blood circulation and stopping bleeding, and astringent and astringent. Golden cherry root mainly contains triterpenes, flavonoids, and tannins, which can treat nocturnal emission, enuresis, dysentery and diarrhea, metrorrhagia, uterine prolapse, hemorrhoids, burns, etc. Commonly used as the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/86
CPCG01N30/02G01N30/8631G01N30/8686
Inventor 张鹏李伏君张英帅白璐伍实花廖端芳李春
Owner ZHUZHOU QIANJIN PHARMA
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