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Collagen cross-linker composition and application thereof

A collagen and cross-linking agent technology, which is applied in the field of medical supplies, can solve the problem of toxic chemical substances entering, and achieve the effects of improving cross-linking efficiency, obvious synergistic effect, and saving raw materials and reaction time

Active Publication Date: 2019-11-15
南京中富先农生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, exogenous toxic chemicals enter the collagen

Method used

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  • Collagen cross-linker composition and application thereof
  • Collagen cross-linker composition and application thereof
  • Collagen cross-linker composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] The preparation of embodiment 1 crosslinking agent composition

[0040] In this example, the crosslinking agent composition solution of the following formula is prepared

[0041] Composition 1:

[0042] Genipin 0.1mg / ml, 0.1mg / ml 5-aminolevulinic acid, 0.05mol / L sodium citrate, 0.02mg / ml imidazole, with PBS as buffer system, pH 8.0.

[0043] Composition 2:

[0044] 0.1mg / ml oxidized sodium alginate, 0.1mg / ml tolylene blue, 0.05mol / L sodium citrate, 0.02mg / ml imidazole, with PBS as buffer system, pH7.5.

[0045] Composition 3:

[0046] 50mg / ml oxidized sodium alginate, 20mg / ml tolylene blue, 0.8mol / L potassium citrate, with PBS as buffer system, pH7.0.

[0047] Control 1:

[0048] Genipin 0.2mg / ml, with PBS as buffer system, pH7.5.

[0049] Control 2:

[0050] 0.2 mg / ml 5-aminolevulinic acid, with PBS as buffer system, pH 7.0.

[0051] Control 3:

[0052]0.1mg / ml oxidized sodium alginate, 0.1mg / ml methylene blue, with PBS as the buffer system, pH7.5.

Embodiment 2

[0053] The acquisition of embodiment 2 collagen biomaterials

[0054] 2.1 Sclera

[0055] New Zealand white rabbits were killed by air embolism, the eyeballs were taken out, and the sclera was separated. After being soaked in dodecyl dimethyl benzyl ammonium bromide solution, then placed in polyethylene glycol octyl phenyl ether solution for incubation, placed in trypsin and EDTA mixed solution for incubation, and placed Incubate in a mixed solution of DNase-1 and RNase-A to obtain decellularized sclera tissue. Slices were made into 1cm×1cm. Obtain the decellularized material.

[0056] 2.2 Vascular tissue

[0057] Fresh and suitable bovine veins were selected and decellularized by a multi-step detergent-enzyme digestion method. Soak in 0.25% trypsin solution for 18 hours, then place in 0.1% SDS for 12 hours, then immerse the tissue in 0.25% trypsin solution for 12 hours, and finally treat it in Dispase at 25°C for 12 hours, and finally place the decellularized cow neck ...

Embodiment 3

[0060] Embodiment 3 cross-linking reaction

[0061] The decellularized material slices obtained in Example 2 were respectively soaked in 20 mL of the solution of Example 1, and put into an 80 mL container. Maintain the temperature of the equilibrium system at 15°C, the pH at 7.5-7.8, and soak for 60 minutes. Subsequently, under the osmotic pressure of 310-330 Osm, the cross-linking reaction was carried out for 60 minutes under the condition of 1000w incandescent light irradiation and oxygen flow. Finally, with 0.25M Na 2 CO 3 / NaHCO 3 buffer solution to adjust the pH of the reaction system to 7, soak and shake for 1 hour, and seal and store for later use.

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Abstract

The invention relates to a collagen cross-linker composition and an application thereof. The composition comprises a photosensitive cross-linker, a chemical cross-linker, citrate and imidazole. By using the cross-linker composition, the chemical crosslinking and photo-oxidation of decellularized collagen can be carried out simultaneously, and synergism is exerted, so that the efficiency of a cross-linking reaction is increased, and a cross-linked collagen medical material with higher quality is obtained.

Description

technical field [0001] The present application relates to the field of medical supplies, in particular to a collagen cross-linking agent composition and its application. Background technique [0002] Collagen is the most widely distributed protein in mammals, which is easy to extract and has good biodegradability and biocompatibility. Collagen is a scaffold material for cell growth, which can promote cell adhesion and induce cell growth and differentiation. It is widely used in tissue engineering research and is widely used in the medical field. [0003] Collagen has been widely used due to its excellent low immune activity, good biocompatibility and biodegradability, but unmodified collagen often suffers from poor thermal stability, excessive degradation rate, low mechanical strength, Volatility and other shortcomings, in many cases can not meet the requirements of use. Therefore, the method of modifying it is usually adopted to effectively improve the thermal stability a...

Claims

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Application Information

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IPC IPC(8): C08K5/00C08K5/092C08K5/3445C08L5/04C08J3/24C08L89/00A61K47/42A61L24/00A61L24/10A61L27/24A61L27/54A61L27/60
CPCA61K47/42A61L24/0015A61L24/0036A61L24/102A61L27/24A61L27/54A61L27/60A61L2300/252A61L2300/412A61L2400/04A61L2430/02A61L2430/16A61L2430/40C08J3/243C08J2389/00C08J2405/04C08K5/0025C08K5/092C08K5/3445C08L5/04
Inventor 戴东升张建伟
Owner 南京中富先农生物科技有限公司
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