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Cladosporium strain lj1 and its application

A strain and Cladospore technology, applied to the Cladosporium strain LJ1 and its application field, can solve problems such as threats to the safety of environmental agricultural products, and achieve the effect of promoting growth

Active Publication Date: 2020-04-10
MICROBIOLOGY RES INST GUANGXI ZHUANG AUTONOMOUS REGION ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In recent decades, the use of chemical fertilizers and pesticides has greatly promoted the increase and stability of grain production. However, the long-term unreasonable use of chemical fertilizers and pesticides has seriously threatened the environment and the safety of agricultural products.

Method used

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  • Cladosporium strain lj1 and its application
  • Cladosporium strain lj1 and its application
  • Cladosporium strain lj1 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The invention provides a strain LJ1 of Cladophiaphora sp., and its preservation number is CGMCC NO.18140. Belonging to the kingdom Fungi, genus Cladophiaphora, unknown species. The endophytic fungus is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, the preservation number is CGMCC No.18140, and the preservation date is July 04, 2019 .

[0026] The above cladospora mold was collected and isolated from the sugarcane rhizosphere soil (N24°01′25.86″, E109°24′59.97″, 95.5 meters above sea level) in the sugarcane field of Chuanshan Town, Liujiang County, Liuzhou City, Guangxi.

[0027] The isolation method adopts the trapping method, specifically using sterile tomato seedlings as trapping plants, planting them in the rhizosphere soil of sugarcane for trapping, and using 1 / 2CM medium to isolate the strain from the root tissue of tomato seedling...

Embodiment 2

[0039] Control effect on banana wilt:

[0040] 1. The control effect of plate:

[0041] (1) Co-cultivation of LJ1 and banana tissue culture seedlings:

[0042] The activated strain LJ1 was inoculated with OMA medium (MgSO4 7H2O 1.0g, KH2PO4 1.5g, NaNO3 1.0g, oat powder 10g, agar powder 11g, distilled water 1000mL) and cultured for 10d, and then selected the sterile Banana tissue-cultured seedlings were transplanted to the colony, and each colony was transplanted with 1 tissue-cultured seedling, transplanted 3 per plate, and named as the treatment group; at the same time, the banana tissue-cultured seedlings were transplanted to the culture plate not inoculated with LJ1 (3 per plate), and named as CK group; put the treatment group and CK group into the incubator for co-cultivation under the same conditions, the culture conditions are: 26°C, light intensity 180μmol / m 2 ·s 2 , the light time is 16h / d; the culture time is 20d.

[0043] (2) Pathogenic medium preparation:

[00...

Embodiment 3

[0074] Promoting effect on tomatoes:

[0075] The specific method includes the following steps:

[0076] (1) Cultivate Cladophyllia strain LJ1 using OMA medium to obtain LJ1 colonies;

[0077] (2) Carry out surface disinfection on the plump tomato seeds, transplant them to the LJ1 colonies cultivated in step (1) after two days of germination, and transplant 1 sterile seedling on each colony, 3 seedlings per dish; The petri dish group inoculated with LJ1 was named as the treatment group; at the same time, the aseptic seedlings were transplanted to the blank OMA medium that was not inoculated with the strain in step (1) simultaneously, and 3 seedlings per dish were named as the CK group;

[0078] (3) Put the treatment group in step (2) and the CK group into the incubator for co-cultivation, the culture temperature is 26°C, and the light intensity is 180 μmol / m 2 ·s 2 , the light time is 16h / d.

[0079] (4) Each treatment of the above step (2) was repeated 4 times; after 14 d...

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Abstract

The invention relates to the field of biotechnology, in particular to Cladosporium strain LJ1 and application thereof. The invention discloses Cladophiaphora sp.LJ1 with the preservation number of CGMCC NO.18140. The strain has the ability to effectively prevent and cure banana wilt. 88.89%, and the pot experiment control effect reached 53.67%. At the same time, the strain has a broad-spectrum growth-promoting effect, and can effectively promote the growth of tomato seedlings (Solanaceae plants) and Dendrobium officinale seedlings (Blue family plants).

Description

【Technical field】 [0001] The invention relates to the field of biotechnology, in particular to Cladosporium strain LJ1 and application thereof. 【Background technique】 [0002] In recent decades, the use of chemical fertilizers and pesticides has greatly promoted the increase and stability of grain production. However, the long-term unreasonable use of chemical fertilizers and pesticides has seriously threatened the environment and the safety of agricultural products. Therefore, it is an urgent need to find new agricultural methods and new technologies that are cost-effective and environmentally compatible to solve the current environmental pollution and food safety crisis. It is of great ecological and economic significance to make full use of the microorganisms in the ecosystem to promote the growth of crops and resist diseases and insect pests to improve the production capacity of crops, protect and improve the agricultural ecological environment, and promote the sustainab...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/02A01N63/30A01P3/00A01P21/00C12R1/645
CPCC12N1/02C12N1/14C12N1/145C12R2001/645
Inventor 农倩谢玲陈艳露张艳廖仕同覃丽萍
Owner MICROBIOLOGY RES INST GUANGXI ZHUANG AUTONOMOUS REGION ACADEMY OF AGRI SCI
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