Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of bacteroides fragilis YCH46 in preparation of drugs for treatment or adjuvant treatment of hypertension

A Bacteroides fragilis, adjuvant therapy technology, applied in the field of medicine, can solve problems such as unreported, and achieve the effects of easy acceptance, low compliance rate and compliance, and mature amplification technology

Pending Publication Date: 2019-11-08
SHANDONG UNIV QILU HOSPITAL
View PDF9 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Bacteroides fragilis (Bacteroides fragilis) YCH46 is a normal colonization bacterium in the human intestinal tract. There is no report on its relationship with hypertension and its effect on hypertension. see the report

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of bacteroides fragilis YCH46 in preparation of drugs for treatment or adjuvant treatment of hypertension
  • Application of bacteroides fragilis YCH46 in preparation of drugs for treatment or adjuvant treatment of hypertension
  • Application of bacteroides fragilis YCH46 in preparation of drugs for treatment or adjuvant treatment of hypertension

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Changes in the intestinal flora of rats in the HSD group

[0047] The feces samples of the rats in the control group and the HSD group were collected, and the total DNA in the feces was extracted by using the improved CTAB method. Universal primers were used to build the library. After the library was built, MiSeq (Illumina) was used for sequencing, and the data obtained by the sequencing was analyzed using QIIME2 software. The correlation analysis results between intestinal microbial genus and systolic blood pressure and diastolic blood pressure figure 1 As shown, the results showed that Bacteroides were closely related to systolic blood pressure (SBP) and diastolic blood pressure (DBP).

[0048] Among them, the general primers of the 16s rRNA gene used for library construction are:

[0049] Upstream primers:

[0050] 5'-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGCCTACGGGNGGCWGCAG-3',

[0051] Downstream primers:

[0052] 5'GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAGGACTAC...

Embodiment 2

[0054] Example 2: Isolation and cultivation of Bacteroides fragilis (Bacteroides fragilis) YCH46 in human intestine

[0055] Take 1g of fresh feces from healthy people and add it to 9mL M2GSC liquid bacterial culture medium to mix well, then use M2GSC liquid bacterial culture medium to dilute 10 concentration gradients in a 10-fold gradient, and take 0.3mL each to spread on M2GSC solid bacteria containing 1.5% agar On the culture medium, cultured at 37° C. for 48 hours in an anaerobic environment, a single colony was taken for 16s rDNA sequencing, and Bacteroides fragilis (Bacteroides fragilis) YCH46 was isolated.

[0056] In addition, the content of Bacteroides fragilis YCH46 in the feces of control group and HSD group rats, hypertensive patients and healthy people was further verified by rtPCR technology, using TB Green Premix Ex Taq (Takara) reagent in CFX96 Real-Time PCR System (Bio -Rad) carry out rtPCR, and the detection result is as follows image 3 As shown, the resul...

Embodiment 3

[0057] Example 3: Effects of Bacteroides fragilis (Bacteroides fragilis) YCH46 on corticosterone synthesis in colon tissue cultured in vitro

[0058] Bacteroides fragilis (Bacteroides fragilis) YCH46 was amplified in M2GSC liquid bacterial culture medium at 37°C for 24 hours by anaerobic shaking to obtain Bacteroides fragilis YCH46 culture solution, which was centrifuged at 5000g for 5 minutes, and the supernatant of the culture solution was filtered out with a 0.2 μm filter B.f.S, the liquid was labeled as B.f.S; similarly, the M2GSC liquid bacterial medium was centrifuged at 5000g for 5 minutes, and the supernatant was filtered and sterilized with a 0.2 μm filter, and the liquid was labeled as M2GSC; under sterile conditions, the normal control group and high-salt induced The colonic tissue of C57BL / 6 male mice in the group, each colonic tissue was fully cut up and divided into two groups, cultured in a sterile 12-well plate, DMEM medium and fetal bovine serum were added to e...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to application of bacteroides fragilis YCH46 in preparation of drugs for treatment or adjuvant treatment of hypertension. A pivotal role of the bacteroides fragilis YCH46 in a human intestinal tract in a hypertension disease is firstly found, the bacteroides fragilis YCH46 can restrain synthesis of intestinal tract corticosterone, and blood pressure is effectively reduced. Inaddition, because the bacteroides fragilis YCH46 is a microorganism normally existing in the intestinal tract, has no side effect on a body, and has the incomparable advantages than other new drugs.The pivotal role of the bacteroides fragilis YCH46 in the hypertension disease is used, drugs, drug compositions, food, health care products or food additives can be prepared, the bacteroides fragilisYCH46 can be used for treating the hypertension disease comprising primary hypertension and salt-sensitive hypertension, and important application value is achieved.

Description

technical field [0001] The invention relates to the application of Bacteroides fragilis YCH46 in the preparation of medicines for treating or assisting hypertension, and belongs to the technical field of medicine. Background technique [0002] Hypertension (hypertension) refers to an increase in systemic arterial blood pressure (systolic and / or diastolic) as the main feature (systolic blood pressure ≥ 140 mm Hg, diastolic blood pressure ≥ 90 mm Hg), which may be accompanied by heart, brain, kidney, etc. Clinical syndrome of functional or organic damage to organs. Hypertension is one of the most common chronic diseases, and its incidence has increased rapidly in recent years. According to the 2017 U.S. Hypertension Guidelines definition (systolic blood pressure ≥130 mmHg, diastolic blood pressure ≥80 mmHg), 45.6% of Americans have high blood pressure. In my country, even according to loose standards (systolic blood pressure ≥ 140mmHg, diastolic blood pressure ≥ 90mmHg), nea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74A61P9/12
CPCA61K35/74A61P9/12
Inventor 张群业闫雪芳张馨洁王哲
Owner SHANDONG UNIV QILU HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com