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Trichoderma virens for preventing and controlling phytophthora capsici and application thereof, and capsicum frutescens l. cultivation method

A technology of pepper phytophthora and cultivation method, which is applied in the field of biological control of plant diseases, can solve ecological and environmental problems, difficult to effectively control and other problems, achieve high-efficiency inhibition, reduce the incidence and degree of disease, and have good biological control applications foreground effect

Active Publication Date: 2019-11-05
ZHEJIANG XINNONG CHEM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These conventional prevention and control technologies have been difficult to effectively control, and are likely to cause ecological and environmental problems

Method used

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  • Trichoderma virens for preventing and controlling phytophthora capsici and application thereof, and capsicum frutescens l. cultivation method
  • Trichoderma virens for preventing and controlling phytophthora capsici and application thereof, and capsicum frutescens l. cultivation method
  • Trichoderma virens for preventing and controlling phytophthora capsici and application thereof, and capsicum frutescens l. cultivation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Isolation, screening and identification of strains.

[0027] 1. Isolation of Trichoderma

[0028] 40 soil samples were collected from 8 field plots in Hangzhou City, Zhejiang Province, which were seriously infested by the pathogenic fungus of Fusarium wilt of pepper. After retrieval, the samples were stored in a refrigerator at 4°C. Take 1g of soil sample and dissolve it in 9ml of sterile water to make a series of soil suspensions. Take 1ml of soil suspension and evenly spread it on Trichoderma screening medium (TSM: 0.2g MgSO 4 7H 2 O; 0.9 g K 2 HPO 4 ; 3.0g glucose; 0.25g chloramphenicol; 0.3g sodium p-dimethylaminobenzenediazepine sulfonate; 0.2g pentachloronitrobenzene; 0.15g Bengal red; 20g agar; add water to 1L), at 27 After culturing at ±1°C for 4 days, the colonies grown on the medium were transferred to potato dextrose medium (PDA) for isolation of single spores. For short-term storage of strains, put them in a 4°C refrigerator. For long-term storage, p...

Embodiment 2

[0037] Detection of antibacterial activity of metabolites.

[0038] The 15 strains that were screened and purified were taken with a hole punch to take a 5mm diameter bacterial cake into a conical flask containing 100ml of PDB medium, and cultured in a ZWY-211B shaker at 27±1°C and 150rpm for 4 days. First filter and centrifuge the mycelium with sterilized gauze to obtain the supernatant containing metabolites. Dilute the resulting supernatant to 50% and 20% concentrations, filter with a bacterial filter with a pore size of 0.22 μm, pour 1 ml of 50% and 25% metabolite solutions together with 10 ml of melted PDA medium into a 9 cm petri dish, and then Insert the Phytophthora capsici cake with a diameter of 5mm and cultivate it at 25°C. The metabolite-free treatment was used as a control, and measurements were taken when control-treated Phytophthora hyphae grew close to the edge of the dish.

[0039] The inhibitory effect test of 15 strains of Trichoderma metabolites on the gr...

Embodiment 3

[0044] Purification and identification of active metabolites.

[0045] 1. Purification and activity testing of active metabolites

[0046] Based on the results of the inhibitory activity, possible active compounds produced by Trichoderma viridans HZA14 were isolated, purified and identified. The screened Trichoderma viridans HZA14 was inoculated into conical flasks containing PDB, and cultured in a shaker for 14 days, using the above method. 2 L of culture fluid was obtained and metabolites were extracted using ethyl acetate. Metabolite residues were obtained by evaporation under reduced pressure. The residue was purified by silica gel column chromatography (particle size: 200-300 mesh), and purified by preparative silica gel TLC (GF254) to obtain four fractions A, B, C and D. A small amount of components A, B, C, and D were dissolved in DMSO (dimethyl sulfoxide) for biological activity determination. 1 ml of each fraction (100 μg / ml) solution was added to a 10 ml Petri di...

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Abstract

The invention discloses trichoderma virens for preventing and controlling phytophthora capsici and an application thereof, and a capsicum frutescens l. cultivation method. The trichoderma virens for preventing and controlling phytophthora capsici is classified and named as trichoderma virens, has the strain number of HZA14, and has the preservation number of CCTCC M 2019484. The trichoderma virenswhich can be used for preventing and controlling phytophthora capsici is obtained by screening, is classified and named as trichoderma virens, has the strain number of HZA14 and has the preservationnumber of CCTCC M 2019484. The trichoderma virens has a high-efficiency inhibition effect on growth of phytophthora capsici mycelia, can be used for preventing and controlling phytophthora capsici, can significantly reduce the incidence rate and the incidence degree of phytophthora capsici and has a good application prospect of biocontrol.

Description

technical field [0001] The invention relates to the technical field of biological control of plant diseases, in particular to a Trichoderma viride used for preventing and treating Phytophthora capsicum, its application and a pepper cultivation method. Background technique [0002] Pepper (Capsicum frutescens L.) has been cultivated in my country for many years and is one of the vegetables with the largest cultivated area in my country, with an annual planted area of ​​1.42 million hectares. In terms of nutritional value, pepper is rich in vitamin C and more antioxidant substances, which can accelerate metabolism, protect the skin, control heart disease, lower cholesterol, prevent cancer and other chronic diseases. In terms of production value, it has strong production adaptability, high yield potential, and good commerciality. It is suitable for long-season, early-early and autumn-delayed high-yield cultivation. [0003] Pepper blight, commonly known as "dead seedling disea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00A01G22/05A01G7/06C12R1/885
CPCA01G7/06A01G22/05C12N1/145C12R2001/885
Inventor 张敬泽刘欣冉
Owner ZHEJIANG XINNONG CHEM CO LTD
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