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Method for rapidly producing transfer factors

A transfer factor and rapid technology, applied in chemical instruments and methods, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve problems such as complex method steps, and achieve the effects of simple reagents, strong practicability, and sufficient cell disruption.

Pending Publication Date: 2019-10-18
商丘美兰生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the purpose of the present invention is to provide a method for rapid production of transfer factor, to solve the problem of complicated steps in the existing method

Method used

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  • Method for rapidly producing transfer factors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A method for rapidly producing transfer factor, comprising the steps of:

[0041] 1) Take fresh pig spleen, wash it, remove fat and film, weigh it after crushing, add distilled water, the amount of distilled water is twice the weight of pig spleen;

[0042] 2) Add the pig spleen and distilled water in step 1) into a masher, and the conditions for mashing are: 8000rpm, homogenate for 5min;

[0043] 3) Add collagenase I to the slurry obtained in step 2), and act at 18°C ​​for 2 hours;

[0044] 4) Adjust the pH of the slurry to 5.0 with 1mol / L hydrochloric acid, act at 18°C ​​for 4 hours, then adjust the pH of the slurry to 8.0 with 1mol / L sodium hydroxide solution, and act at 18°C ​​for 6 hours;

[0045] 5) Use 1mol / L hydrochloric acid to adjust the pH of the slurry to 6.5, ultrasonically crush for 15 minutes, and repeat once every 5 minutes; when ultrasonically crushing, the power of the ultrasonic wave is 19.5KHz, and the temperature is 8°C;

[0046] 6) Centrifuge the...

Embodiment 2

[0049] A method for rapidly producing transfer factor, comprising the steps of:

[0050] 1) Take fresh pig spleen, wash it, remove fat and film, weigh it after crushing, add distilled water, the amount of distilled water is 3 times the weight of pig spleen;

[0051] 2) Add the pig spleen and distilled water in step 1) into the masher, and the conditions for mashing are: 10000rpm, homogenate for 3min;

[0052]3) Add collagenase I to the slurry obtained in step 2), and act at 25°C for 1 hour;

[0053] 4) Use 1mol / L hydrochloric acid to adjust the pH of the slurry to 6.0, act at 25°C for 2h, then use 1mol / L sodium hydroxide solution to adjust the pH of the slurry to 9.0, and act at 25°C for 3h;

[0054] 5) Use 1mol / L hydrochloric acid to adjust the pH of the slurry to 7.2, ultrasonically crush for 30 minutes, and repeat once every 10 minutes; when ultrasonically crushing, the power of the ultrasonic wave is 20.5 KHz, and the temperature is 2°C;

[0055] 6) Centrifuge the slurry...

Embodiment 3

[0058] A method for rapidly producing transfer factor, comprising the steps of:

[0059] 1) Take fresh pig spleen, wash it, remove fat and film, weigh it after crushing, add distilled water, the amount of distilled water is twice the weight of pig spleen;

[0060] 2) Add the pig spleen and distilled water in step 1) into the mashing machine, the conditions for mashing are: 9000rpm, homogenization for 4min;

[0061] 3) Add collagenase I to the slurry obtained in step 2), and act at 20°C for 2 hours;

[0062] 4) Adjust the pH of the slurry to 6.0 with 1mol / L hydrochloric acid, act at 20°C for 3h, then adjust the pH of the slurry to 9.0 with 1mol / L sodium hydroxide solution, and act at 20°C for 5h;

[0063] 5) Use 1mol / L hydrochloric acid to adjust the pH of the slurry to 7.0, ultrasonically crush for 20 minutes, and repeat once at an interval of 8 minutes; when ultrasonically crushing, the power of the ultrasonic wave is 20KHz, and the temperature is 5°C;

[0064] 6) Centrifug...

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Abstract

The invention belongs to the field of veterinary biological products, and particularly relates to a method for rapidly producing transfer factors. The method includes the main steps of tissue homogenization, enzymatic hydrolysis, acid-base digestion, ultrasonic extraction, centrifugation, ultrafiltration, and the like. Compared with the traditional freeze-thaw process, the method has the advantages that the reagents adopted by the method are simple, the cost is low, cells are more fully broken, the production cycle is short, a lot of manpower and material resources are saved, the cost is significantly reduced, and the method is suitable for large-scale production of economic animal products.

Description

technical field [0001] The invention belongs to the field of veterinary biological products, and in particular relates to a method for rapidly producing transfer factor. Background technique [0002] Transfer factor is a dialyzable small-molecule polypeptide and nucleotide complex released by immune-competent T lymphocytes in human or animal white blood cells. Studies have found that it has a good preventive effect on various animal diseases. The preparation of transfer factor mostly adopts the method of repeated freezing and thawing to disrupt cells, but this method has disadvantages such as long operation time (6-8 days), easy to be infected with bacteria, and unstable active ingredients. [0003] Chinese patent ZL201510782710.9, a process for preparing transfer factor capsules, introduces a process for preparing transfer factor capsules. After filtering, filtering, sterilizing and freeze-drying, the transfer factor freeze-dried powder is obtained, and starch, microcrysta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/26A61K35/17A61K38/17C07K1/36C07K14/47
CPCA61K35/26A61K35/17A61K38/1709C07K14/47C07K1/36
Inventor 王彦辉李自波刘兵李国辉焦金英张宁孟祥李冰姬星宇
Owner 商丘美兰生物工程有限公司
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