Breeding method for orange Romanesco Broccoli hybridization seeds

A pagoda flower and hybrid technology, which is applied in the field of selection and breeding of orange pagoda cauliflower hybrids, can solve problems such as the emergence of orange pagoda cauliflower varieties or breeding research work, etc., and achieves reduced seed production costs, high homozygosity, and comprehensive traits. excellent effect

Active Publication Date: 2019-10-18
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Ordinary pagoda cauliflower seeds come from abroad, and there is no emergence or breeding research of orange pagoda cauliflower varieties at home and abroad

Method used

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  • Breeding method for orange Romanesco Broccoli hybridization seeds

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Comparison scheme
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Embodiment 1

[0024] Such as figure 1 Shown, present embodiment provides a kind of selection method of orange pagoda cauliflower hybrid, specifically comprises the following steps:

[0025] 1) Screening of germplasm resources. Since 2010, 7 pagoda-shaped cauliflower germplasm resources and 2 orange cauliflower germplasm resources have been collected. Through the observation and identification of agronomic characters, 1 germplasm of male sterile pagoda-type cauliflower, 3 fertile pagoda-type cauliflowers and 2 orange-colored cauliflower germplasms were screened out.

[0026] 2) Male sterility source selection. The male sterile pagoda-type cauliflower hybrid, denoted as bj1841, showed normal petals and nectaries, and it was used as a male sterile breeding material.

[0027] 3) Selection and breeding of recurrent parents. Using fertile pagoda-type broccoli hybrids as donors, denoted as RCK01, 13 strains of double haploid series were obtained through microspore culture, denoted as DH1633, a...

Embodiment 2

[0032] Embodiment 2 parent breeding

[0033] Because pagoda cauliflower needs to be cut many times and cultivated over winter, it has a strong winter nature, so parents need to be bred in a greenhouse to prevent the wounds after cutting from being infected by rainwater. Because of the self-compatibility of the recurrent parent, seeds can be obtained through assisted pollination by releasing bees in a separate shed; the male sterile line is planted in a separate shed with a ratio of 1:1 to the male sterile line and the recurrent parent, which needs to be pollinated by releasing bees. Seeds are obtained by backcrossing; the male parent is a self-incompatible DH line, which needs to be pollinated manually at the flowering stage in a greenhouse to obtain seeds.

Embodiment 3

[0034] Embodiment 3 hybrid seed production

[0035] If a large amount of seed production is required for the first-generation hybrid species, it is possible to find a suitable area for isolated seed collection in open-air sun borders, and under normal circumstances, isolated seed production can be carried out in a greenhouse.

[0036] 1) Choose a planting greenhouse: According to the production area, choose an ordinary plastic greenhouse, glass greenhouse or solar greenhouse. It is best not to plant cruciferous crops in the previous work, or the greenhouse soil has been sterilized. Apply base fertilizer and borax before transplanting.

[0037] 2) Ratio of sowing and planting: the parents raise seedlings and transplant, and plant according to the ratio of female parent: male parent 2:1 or 3:2. Because the growth periods of the female parent and the male parent are similar, they can be sown and transplanted at the same time. Taking Hangzhou as an example, the sowing period of ...

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Abstract

The invention discloses a breeding method for orange Romanesco Broccoli hybridization seeds. An orange Romanesco DH line obtained through microspore culture or through traditional breeding selling homozygosis or a selfing pure line is adopted as a male parent, a common Romanesco Broccoli ogura cytoplasmic male sterile line obtained through multi-generation backcross transformation is adopted as afemale parent, and the new species of the first-filial generation of the orange Romanesco Broccoli is obtained through the hybridization way. The orange Romanesco Broccoli obtained through the methodis prepared from the ogura cytoplasmic male sterile line, the recurrent parent is self-compatible, honeybee pollination can be performed to perform coeno-species and sterile line propagation, and theseed manufacturing cost is greatly lowered.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a method for breeding orange pagoda cauliflower hybrids. Background technique [0002] Pagoda cauliflower (Brassica oleracea L.var.botrytis cv.romanesco), also known as Roman cauliflower, was first recorded in Italy in the 16th century. It has been introduced in my country since the end of the 20th century, and it is cultivated in a small amount in my country as a special dish. Pagoda cauliflower is rich in vitamin C, K, crude fiber and carotenoids, and its curds are arranged in a fractal Fibonacci sequence, which is both nutritious and ornamental. [0003] Ordinary pagoda cauliflower seeds come from abroad, and there is no emergence or breeding research work of orange pagoda cauliflower varieties at home and abroad. The germplasm resources of common pagoda cauliflower are very few, and most of them are sterile hybrids, and the available resources are even ra...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 顾宏辉赵振卿王建升虞慧芳盛小光沈钰森
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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