Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A strain of Streptomyces alginolyticus n1-32, its microecological preparation and its preparation method

A technology of Streptomyces alginolyticus and microecological preparations, which are applied to the microecological preparations and their preparations, in the field of Streptomyces alginolyticus N1-32, and can solve the problems of Streptomyces alginolyticus and the like that have not yet been seen

Active Publication Date: 2022-06-03
乾胜(深圳)科创集团有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] But so far, there is no report on the application of Streptomyces alginolyticus in the prevention and control of aquaculture diseases.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A strain of Streptomyces alginolyticus n1-32, its microecological preparation and its preparation method
  • A strain of Streptomyces alginolyticus n1-32, its microecological preparation and its preparation method
  • A strain of Streptomyces alginolyticus n1-32, its microecological preparation and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0068] Example 1: The resistance of Streptomyces alginolyticus to the fish pathogen Aeromonas viridis after feeding grass carp into the feed

[0069] 270 healthy grass carp (10-12.5g) for the test were randomly divided into 9 groups, raised in a laboratory cylindrical breeding box (diameter 1m, water volume 300L), and fed a feed equivalent to 2% of the fish weight every day. Feed for 2 weeks to ensure the health of grass carp, and test after 24 hours of fasting. During the experiment, the light cycle was 12L / 12D, the water temperature was 25±2°C, the pH was about 7.2±0.5, and the feed was Tongwei 156 extruded pellets purchased from Tongwei Co., Ltd.

[0070] Experimental design: the first group: fed with sterilized fish feed, the second group: fed with a concentration of 1 × 10 7 cfu / g of N1-32 strain live bacteria feed, the third group: 1 × 10 was added to the feed 9 cfu / g N1-32 strain live bacteria feed; three replicates were set for each group. After feeding for 28 days, ...

example 2

[0075] Example 2: Prevention and control of fish Aeromonas viridis disease by Streptomyces alginolyticus agent by water splashing

[0076] 270 healthy grass carp (10-12.5g) were randomly divided into 9 groups and raised in a laboratory cylindrical breeding box (diameter 1m, water volume 300L). Feed for 2 weeks to ensure the health of grass carp, and test after 24 hours of fasting. During the experiment, the light cycle was 12L / 12D, the water temperature was 25±2°C, the pH was about 7.2±0.5, and the feed was Tongwei 156 extruded pellets purchased from Tongwei Co., Ltd.

[0077] Experimental design: Group 1: Streptomyces alginolyticus N1-32 was added to the culture water, so that the final concentration was controlled at 1 × 10 5cfu / mL, the spraying cycle is 28 days, and the concentration of 1×10 is injected into grass carp on the 29th day. 8 cfu / mL of Aeromonas viridis, the injection dose is 0.2mL / tail; the second group: add Streptomyces alginolyticus N1-32 to the culture wat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

A strain of Streptomyces alginolyticus N1-32, its microecological preparation and its preparation method. The Streptomyces alginolyticus N1‑32 is preserved in the China Center for Type Culture Collection, and the strain preservation number is CCTCCNO: M 2019381. The Streptomyces alginolyticus N1‑32 can effectively inhibit the growth of common fish pathogens, especially Aeromonas victoria. The probiotics of Streptomyces alginolyticus N1‑32 acted on liver cells of grass carp showed that the bacteria had no toxic effect on fish. By using the probiotics of Streptomyces alginolyticus N1‑32 as a feed additive to feed freshwater fish such as grass carp and crucian carp, the results show that the growth rate and bait efficiency of grass carp are improved, and the immunity of freshwater fish can be enhanced at the same time, thus Significantly improve the resistance of grass carp and other freshwater fish to fish pathogenic bacteria.

Description

technical field [0001] The invention relates to the field of microecological preparations, in particular to a strain of Streptomyces alginolyticus N1-32, a microecological preparation thereof and a preparation method thereof. Background technique [0002] At present, antibiotics are mainly used in the prevention and control of diseases in fish farming. Antibiotics can control the outbreak of diseases, but they can also destroy the microbial community structure normally existing in the aquaculture water environment and fish intestines, resulting in fish exposure to pathogenic microorganisms. The reduction of resistance and the generation of drug-resistant pathogens; in addition, the extensive use of antibiotics will leave aquatic product drug residues, endangering human health, and at the same time destroying the ecological environment, which is not conducive to the development of ecological aquaculture. The aquatic microecological preparations have the advantages of no toxic...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18A23K50/80A61K35/741A61P31/04C12R1/465
CPCC12N1/20A23K10/18A23K50/80A61K35/741A61P31/04C12R2001/465C12N1/205Y02A40/81
Inventor 夏立秋李艳平易敢峰丁学知
Owner 乾胜(深圳)科创集团有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products