A strain of Streptomyces alginolyticus n1-32, its microecological preparation and its preparation method
A technology of Streptomyces alginolyticus and microecological preparations, which are applied to the microecological preparations and their preparations, in the field of Streptomyces alginolyticus N1-32, and can solve the problems of Streptomyces alginolyticus and the like that have not yet been seen
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example 1
[0068] Example 1: The resistance of Streptomyces alginolyticus to the fish pathogen Aeromonas viridis after feeding grass carp into the feed
[0069] 270 healthy grass carp (10-12.5g) for the test were randomly divided into 9 groups, raised in a laboratory cylindrical breeding box (diameter 1m, water volume 300L), and fed a feed equivalent to 2% of the fish weight every day. Feed for 2 weeks to ensure the health of grass carp, and test after 24 hours of fasting. During the experiment, the light cycle was 12L / 12D, the water temperature was 25±2°C, the pH was about 7.2±0.5, and the feed was Tongwei 156 extruded pellets purchased from Tongwei Co., Ltd.
[0070] Experimental design: the first group: fed with sterilized fish feed, the second group: fed with a concentration of 1 × 10 7 cfu / g of N1-32 strain live bacteria feed, the third group: 1 × 10 was added to the feed 9 cfu / g N1-32 strain live bacteria feed; three replicates were set for each group. After feeding for 28 days, ...
example 2
[0075] Example 2: Prevention and control of fish Aeromonas viridis disease by Streptomyces alginolyticus agent by water splashing
[0076] 270 healthy grass carp (10-12.5g) were randomly divided into 9 groups and raised in a laboratory cylindrical breeding box (diameter 1m, water volume 300L). Feed for 2 weeks to ensure the health of grass carp, and test after 24 hours of fasting. During the experiment, the light cycle was 12L / 12D, the water temperature was 25±2°C, the pH was about 7.2±0.5, and the feed was Tongwei 156 extruded pellets purchased from Tongwei Co., Ltd.
[0077] Experimental design: Group 1: Streptomyces alginolyticus N1-32 was added to the culture water, so that the final concentration was controlled at 1 × 10 5cfu / mL, the spraying cycle is 28 days, and the concentration of 1×10 is injected into grass carp on the 29th day. 8 cfu / mL of Aeromonas viridis, the injection dose is 0.2mL / tail; the second group: add Streptomyces alginolyticus N1-32 to the culture wat...
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