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Identification method for mesocotyl elongation capability of dry direct seeded rice

An identification method, dry direct seeding technology, applied in rice cultivation, botanical equipment and methods, soilless cultivation, etc., can solve the problems of poor repeatability of experimental results, achieve shortened test period, clear formula, improve repeatability and The effect of stability

Active Publication Date: 2019-10-15
AGRI GENOME INST OF SHENZHEN CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many QTLs controlling mesocotyl elongation have been mapped in different studies so far, but due to the significant interaction between mesocotyl elongation and environmental conditions, the reproducibility of the experimental results is poor

Method used

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  • Identification method for mesocotyl elongation capability of dry direct seeded rice
  • Identification method for mesocotyl elongation capability of dry direct seeded rice
  • Identification method for mesocotyl elongation capability of dry direct seeded rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The mesocotyls of the same rice population (208 rice germplasms introduced by International Rice) were identified by hydroponics, sand culture, soil culture (field loam) and nutrient soil (Germany K brand-TS1) culture methods elongation ability. Two independent repetitions were set up for each culture method. The specifications of the hole plates used are 54cm (length) × 28cm (width) × 11cm (height), 50 holes.

[0021] a. In the hydroponics method, spread gauze on the bottom of each hole, select 15 full seeds and spread them on the gauze, place the hole tray in the tray and fill the tray with water until the water surface is less than 1cm above the seeds Then use tinfoil paper to seal the plugs and trays and shield them from light. Finally, place the sealed plugs in an artificial climate chamber at 30°C. After culturing for 7 days, use a ruler to measure the mesocotyl length.

[0022] b. Sand culture method: Before sowing, fill each hole in the hole with sand to a dis...

Embodiment 2

[0030] The variation of mesocotyl length in 395 MAGIC rice populations was detected by the nutrient soil culture method, and the specific operation steps were the same as the above-mentioned nutrient soil culture method.

[0031] Analysis of test results:

[0032] The variation range of mesocotyl length in MAGIC population was 0–4.8 cm, and the distribution was uniform and continuous, which was basically consistent in the two experimental repetitions ( image 3 ). At the same time, the mesocotyl length was highly correlated in two experimental replicates (R 2 =0.8459)( Figure 4 ), basically consistent with the correlation results in Example 11.

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Abstract

The invention discloses an identification method for mesocotyl elongation capability of dry direct seeded rice. The mesocotyl elongation capability of rice is identified with a nutritional soil culture method under the conditions of high temperature and low humidity (35 DEG C, RH=50%-60%), the emergence situation is observed every day, the sequence of emergence of different varieties is recorded,and mesocotyl length is measured on the third day after emergence of the first variety; seedlings are taken out of trays in sequence, washed clean, laid flat on a flat plate with calibrations in sequence and photographed; after all varieties are photographed in sequence, the mesocotyl length of each variety is measured by Image J software by setting a scale according to calibrations on the flat plate. By the application of the method in identification of the mesocotyl elongation capability of dry direct seeded rice, the test period can be obviously shortened, the phenotype identification efficiency of the mesocotyl is improved, and test errors caused by human factors are reduced. Meanwhile, repeatability and stability of identification results of the mesocotyl elongation capability of thedry direct seeded rice are greatly improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for identifying the mesocotyl elongation ability of dry-directed rice. , Background technique [0002] Dry direct-seeding rice has a series of advantages such as water saving, labor saving, and suitable for mechanized cultivation. However, in the production of dry direct-seeding rice, due to insufficient rooting depth, lodging phenomenon often occurs in the later stage of rice growth. To solve this problem, in In production, it is often necessary to increase the sowing depth. Deep sowing is conducive to the rooting of rice, improving the water and nutrient absorption capacity of the root system, and improving the lodging resistance of rice. Insufficient seedlings and weed control problems. And under the condition of deep sowing, the seed germination lasts for a long time, during which the seedlings cannot carry out photosynthesis, which will easily cause the growth of the...

Claims

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Application Information

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IPC IPC(8): A01G22/22A01G31/00
CPCA01G31/00A01G22/22
Inventor 刘宏岩詹俊辉叶国友
Owner AGRI GENOME INST OF SHENZHEN CHINESE ACADEMY OF AGRI SCI
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