Soil infiltration system metabonomics analysis method based on UPLC-MS

Abstract

The invention discloses a soil infiltration system metabonomics analysis method based on UPLC-MS. The method comprises the following process steps of 1) carrying out sample preparation: collecting a soil sample, and performing UPLC-MS detection analysis by adopting a liquid chromatography-electrospray time-of-flight mass spectrometry system after pretreatment; 2) obtaining sample data through theUPLC-MS detection technology, and carrying out extraction, noise filtering, deconvolution, alignment and normalization on the sample data; 3) carrying out multivariate statistical analysis on the dataafter the normalization processing; 4) screening and identifying and analyzing the differential metabolites; 5) analyzing a microorganism metabolic pathway; and 6) carrying out redundant (RDA) analysis of metabolites and environmental factors. The soil infiltration system metabonomics analysis method based on UPLC-MS is simple and easy to implement, can comprehensively reflect the difference condition of the soil microorganism metabolites, is reasonable and reliable in model design, and provides a quick, efficient and accurate analysis method for researching soil system microorganism metabonomics.

Description

technical field [0001] The invention belongs to the field of biotechnology, relates to the research on microbial metabolism in a soil infiltration system, and provides a method for research on microbial metabolic mechanism. Background technique [0002] Soil infiltration system (SIS) is a sewage ecological treatment technology with low investment, low energy consumption and low operating cost. SIS contains a large number of various microorganisms. The action of microorganisms is an important way for SIS to decontaminate. The metabolic process and product characteristics of microorganisms are an important basis for qualitatively describing the pollutant removal process. [0003] Microbial metabolomics refers to the simultaneous qualitative and quantitative analysis of all low-molecular-weight metabolites existing inside and outside the cell in an unbiased and reproducible manner at a specific time point in microbial cell growth or growth cycle. , analysis of metabolic pathwa...

AI Technical Summary

Problems solved by technology

[0005] However, at present, the research on the ecological characteristics of microorganisms in SIS still stays in the traditional microbial analysis method. When using traditional molecular biology methods to study SIS, when the system is disturbed, the effluent water quality and biological flora cannot be adjusted immediately.

This simple traditional microbial analysis method cannot accurately reflect the complete biological information of the SIS, and it is not reliable to intuitively determine whether the SIS is healthy or not.

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