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Culture medium for betula platyphylla embryogenic callus induction, method for embryogenic callus induction and cultivation method for tissue culture seedlings

A technology of embryogenic callus and induction medium, which is applied in the cultivation of tissue culture seedlings, the embryogenic callus induction medium of birch, and the field of inducing embryogenic callus. Effect

Inactive Publication Date: 2019-10-11
NORTHEAST FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

There is no report on the induction of somatic embryos in birch in the prior art

Method used

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  • Culture medium for betula platyphylla embryogenic callus induction, method for embryogenic callus induction and cultivation method for tissue culture seedlings
  • Culture medium for betula platyphylla embryogenic callus induction, method for embryogenic callus induction and cultivation method for tissue culture seedlings
  • Culture medium for betula platyphylla embryogenic callus induction, method for embryogenic callus induction and cultivation method for tissue culture seedlings

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preparation example Construction

[0034] The preparation method of the induction medium is not particularly limited in the present invention, and the conventional preparation method of the medium well known in the art can be used.

[0035] The invention provides a method for inducing embryogenic callus based on birch immature embryos, comprising:

[0036] Select white birch immature embryos for disinfection, inoculate the sterilized white birch immature embryos on the embryogenic callus induction medium described in the above technical scheme, and carry out light-dark cycle culture at 24-26°C for 16h / 8h, subculture After cultured for 4 weeks, embryogenic callus was obtained.

[0037] In the present invention, the disinfection method preferably rinses the immature seeds with water, soaks them in an alcohol solution with a volume concentration of 70% for 30 seconds, rinses them with water, and then mixes and disinfects them with a sodium hypochlorite solution with a mass concentration of 3% for 10 minutes. , ri...

Embodiment 1

[0049] Birch embryogenic callus induction medium: based on MS medium, containing 2,4-D2mg / L, NAA0.2mg / L, agar 5.5g / L and sucrose 40g / L; among them, embryogenic callus The pH value of wound tissue induction medium was 5.8. The above medium was divided into 100ml Erlenmeyer flasks and autoclaved at 121°C for 15min.

Embodiment 2

[0051] 1 Embryogenic callus induced by immature embryos of birch

[0052] 1.1 Materials

[0053] Collect the immature seeds from pollination on May 4th to about one month after June 4th from the adult trees of white birch for disinfection. Cones and immature seeds are as follows: image 3 .

[0054] 1.2 Induction of embryogenic callus

[0055] The explants after the disinfection (disinfection method is the same as comparative example 1) are inoculated to the -1 Agar and 2.0mg·L -1 2,4-D and 0.2mg·L -1 Cultured in the dark in MS medium of NAA, subcultured once every 4 weeks, and placed in a place where the temperature was adjusted to 25±1°C, the light-dark cycle was 16h / 8h, and the light intensity was 36μmol·m -2 ·s -1 cultured in a culture room, and the subculture period was 4 weeks.

[0056] The results showed that the contamination rate of explants was 0%, and the callus induction rate of immature seeds within one month of pollination reached over 85% (Table 1). The ...

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Abstract

The invention provides a culture medium for betula platyphylla embryogenic callus induction, a method for embryogenic callus induction and a cultivation method for tissue culture seedlings, and belongs to the technical field of plant tissue culture. The provided culture medium for betula platyphylla embryogenic callus induction takes an MS culture medium as a basic culture medium and comprises 2 mg / L of 2,4-D, 0.2 mg / L of NAA, 5.5 g / L of agar and 40 g / L of saccharose, and the pH value of the culture medium for betula platyphylla embryogenic callus induction is 5.8. By using the culture medium,the callus induction rate is 90% or above, and the embryogenic callus induction rate is 19% or above.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a white birch embryogenic callus induction medium, a method for inducing embryogenic callus and a method for cultivating tissue culture seedlings. Background technique [0002] White birch (Betula platyphalla) belongs to Betaceae, Betula genus, 15-20m high and 50cm diameter at breast height. It is mainly distributed in the back of the Northeast region, and is produced in forest areas such as the Greater and Lesser Khingan Mountains and the Wanda Mountains. In the mountainous areas such as Zhangguangcai Mountain and Laoye Mountain in the northeast, Northeast white birch is the main species. White birch and Northeast white birch are native tree species in Northeast China, with graceful posture, smooth and white bark, reddish-brown twigs, and high ornamental value. Birch is a strong positive tree species, light-loving, cold-resistant, water-resistant, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李成浩杨静莉王瀚增李丹丹李文龙
Owner NORTHEAST FORESTRY UNIVERSITY
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