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Fluorescence detection method for antiepileptic drug-tiagabine hydrochloride (TGB)

A technology for tiagabine hydrochloride and antiepileptic drugs, which is applied in the field of fluorescence detection of antiepileptic drugs --- tiagabine hydrochloride, which can solve the problems of high analysis cost, complicated analysis process, and long detection time, and achieve good selectivity , high sensitivity, convenient and fast detection process

Active Publication Date: 2019-10-08
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

The detection of TGB by high performance liquid chromatography is a classic detection method with good linearity and high accuracy, but this method also has some shortcomings and deficiencies, such as: high analysis cost, complicated analysis process, long detection time, etc.

Method used

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  • Fluorescence detection method for antiepileptic drug-tiagabine hydrochloride (TGB)
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  • Fluorescence detection method for antiepileptic drug-tiagabine hydrochloride (TGB)

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Embodiment

[0031] A kind of antiepileptic drug of the present invention---the fluorescent detection method of tiagabine hydrochloride (TGB), comprises the following steps:

[0032] (1) Establish a fluorescent probe --- PDI-HSA

[0033] Dissolve PDI and HSA in 50% ethanol solution and ultrapure water respectively, and make 1 mM mother solution. A certain volume of the above two mother solutions was mixed and diluted to a concentration of 5 μM and 40 μM respectively to obtain the fluorescent probe PDI-HSA. Specifically include the following steps:

[0034] ① Take a certain volume of the above-mentioned PDI mother liquor and dilute it to 20 μM with ultrapure water, and measure the maximum excitation wavelength of PDI by ultraviolet absorption spectroscopy.

[0035] ② Take a certain volume of PDI mother liquor and dilute it to 5 μM with ultrapure water, measure the fluorescence intensity with a fluorescence spectrum analyzer at the maximum excitation wavelength, then add a series of HSA so...

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Abstract

The invention discloses a fluorescence detection method for an antiepileptic drug-tiagabine hydrochloride (TGB). Through study on interaction among dye perylene diimide derivative (PDI), human serum albumin (HSA) and the tiagabine hydrochloride (TGB), the fluorescence of the PDI is found to be quenched by the HSA, and the study indicates that the PDI is bound at a site II of the HSA, the TGB is able to compete for being bound at the site II of the HSA, the PDI is beaten at the site II of the HSA, and fluorescence recovery is thus realized. On the basis, the invention provides a TGB fluorescence detection method. With the PDI-HSA as a probe, under effects of the TGB, fluorescence recovery is realized, and the TGB is thus detected. The method has the advantages of original innovation, simpledetection process, mild experimental condition, high sensitivity, good selectivity, fast detection time and low detection limit and the like, and has good social value and application prospects.

Description

technical field [0001] The invention belongs to the field of drug detection, in particular to a fluorescence detection method of antiepileptic drug tiagabine hydrochloride (TGB). Background technique [0002] Epilepsy is one of the most common diseases of the nervous system. The main clinical manifestations are: different degrees of disturbance in sensation, movement, behavior and autonomic nerves during seizures, which seriously threaten human health and the quality of life of patients. At present, the main and most commonly used treatment for epilepsy is still drug therapy. Tiagabine hydrochloride (TGB) is a new type of antiepileptic drug, its mechanism of action is to block the reuptake of γ-aminobutyric acid (GABA) by neurons and glial cells, and increase the synaptic site γ-aminobutyric acid (GABA) level to achieve anticonvulsant effect. However, studies have shown that excessive use of TGB can produce a series of side effects, such as dizziness, weakness, tension, ab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 邹振廖小豆杨荣华闫奇杨华
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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