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A kind of malt root protein hydrolyzate with inhibitory alpha-glucosidase activity and preparation method thereof

A protein hydrolyzate and glucosidase technology, applied in the field of malt root protein hydrolyzate and its preparation, to achieve a good effect of inhibiting the activity of α-glucosidase

Active Publication Date: 2022-03-25
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is no relevant report on the inhibition of α-glucosidase activity by malt root protein hydrolyzate

Method used

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  • A kind of malt root protein hydrolyzate with inhibitory alpha-glucosidase activity and preparation method thereof
  • A kind of malt root protein hydrolyzate with inhibitory alpha-glucosidase activity and preparation method thereof
  • A kind of malt root protein hydrolyzate with inhibitory alpha-glucosidase activity and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Use a pulverizer to make the malt root into dry powder, pass through a 60-mesh sieve to obtain the malt root dry powder, add deionized water according to a material-to-liquid ratio of 1:10, and mix evenly, wherein the mass of the malt root dry powder is 10g, and the volume of deionized water is 10 g. To 100 mL, Suspension 1 was formed. 0.4% alkaline protease of the mass of the dry powder of malt root was added to the suspension 1, and the enzymatic hydrolysis reaction was carried out at a constant temperature of 50°C, and the enzymatic hydrolysis time was 3 h to obtain the suspension 2. Then heat the suspension 2, the heating temperature is 90°C, and the heating time is 15min. After cooling to room temperature, centrifugation is performed at a low temperature of 4°C. The supernatant was obtained to obtain extract 1. The extract 1 was separated by filtration, the filtrate was taken, and then the pH of the filtrate was adjusted to 7 to obtain the extract 2. AB-8 macropo...

Embodiment 2

[0039] Use a pulverizer to make the malt root into dry powder, pass through an 80-mesh sieve to obtain the malt root dry powder, add deionized water according to a material-to-liquid ratio of 1:15, and mix evenly, wherein the mass of the malt root dry powder is 10g, and the volume of deionized water is 10 g. 150 mL, Suspension 1 was formed. 0.6% alkaline protease by mass of the dry powder of malt root was added to the suspension 1, and the enzymatic hydrolysis reaction was carried out at a constant temperature of 50° C., and the enzymatic hydrolysis time was 4 h to obtain the suspension 2. Then heat the suspension 2, the heating temperature is 95°C, the heating time is 10min, and after cooling to room temperature, centrifugation is carried out at a low temperature of 7°C. The supernatant was obtained to obtain extract 1. The extract 1 was separated by filtration, the filtrate was taken, and then the pH of the filtrate was adjusted to 7.5 to obtain the extract 2. AB-8 macropo...

Embodiment 3

[0041] Use a pulverizer to make the malt root into dry powder, pass through an 80-mesh sieve to obtain the malt root dry powder, add deionized water according to a material-to-liquid ratio of 1:20, and mix well, wherein the mass of the malt root dry powder is 10g, and the volume of deionized water is 10 g. 200 mL, Suspension 1 was formed. 1% alkaline protease by mass of the dry powder of malt root was added to the suspension 1, and the enzymatic hydrolysis reaction was carried out at a constant temperature of 55°C, and the enzymatic hydrolysis time was 4 h to obtain the suspension 2. Then heat the suspension 2, the heating temperature is 100°C, and the heating time is 10min. After cooling to room temperature, centrifugation is performed at a low temperature of 10°C. solution to obtain extract 1. The extract 1 was separated by filtration, the filtrate was taken, and then the pH of the filtrate was adjusted to 7.5 to obtain the extract 2. AB-8 macroporous resin was used to ads...

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Abstract

The invention discloses a malt root protein hydrolyzate with the activity of inhibiting α-glucosidase and a preparation method thereof. The preparation method comprises processes such as crushing and sieving malt root, enzymatic hydrolysis with protease, low-temperature high-speed centrifugation, filtration and separation, macroporous resin adsorption, column chromatography and freeze-drying to obtain a malt root with activity of inhibiting α-glucosidase. protein hydrolyzate. The polypeptide content of the hydrolyzate is more than 85% (mass percentage), and the inhibition rate to α-glucosidase is more than 55%. The preparation process provided by the invention is simple, and the whole process flow can meet the requirements of food grade, and can be applied in the fields of medicine, health products and food.

Description

technical field [0001] The invention belongs to the field of barley deep processing and high value, in particular to a malt root protein hydrolyzate with α-glucosidase inhibiting activity and a preparation method thereof. Background technique [0002] In the existing patents on substances that inhibit α-glucosidase activity, the raw materials are mostly derived from precious Chinese medicinal materials, deep-sea animals and plants, and herbs affected by seasons and regions. First, it is difficult to obtain materials and the cost is high. Second, The output is low and cannot meet the market demand. Barley is a major food and forage crop and the main raw material for brewing beer. Malt root is the main by-product of malt production in the beer industry. After the barley is germinated, it is dried and obtained through a root removal process. In actual production, the amount of by-product malt root accounts for about 3-4% of the total barley feeding amount. my country is a bi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/14C07K1/34C07K1/16
CPCC12P21/06C07K1/14C07K1/34C07K1/16
Inventor 袁尔东常博任娇艳沈佳奇
Owner SOUTH CHINA UNIV OF TECH
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